FastQCFastQC Report
Thu 26 May 2016
SRR1546223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546223_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1392194
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207841.4928953867061632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28500.20471284892766384No Hit
TGTGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT20840.1496917814614917No Hit
TAAGTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA20600.14796788378631137No Hit
GGGCAAGGGATAAAACTGGGTCACATAATAATCATATTCCAACTTTTGTA19730.1417187547137827No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18920.13590060006004911No Hit
AAGCTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18170.13051341982511058No Hit
ACGAAAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT17550.1260600174975614No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT17290.12419246168278272No Hit
ATTATGGGGAGCTAAAATTTTTTTAAAATGGAATTTTGCTTCCACTATTT17000.12210941865860647No Hit
TGATTTGGGGATGAAGATTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGA16910.12146295703041386No Hit
TAGTTAGGGCTATTAATGATATTAAAATCCCAACTATCCAAAGAATATCC16690.1198827174948319No Hit
GGGGAGGGGGGCATAAGTAAATAGAATTAAGTAAATTACCTTACTATTTT16350.11744052912165975No Hit
AGCATAGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA15730.11298712679411059No Hit
GGATTTGGGATCCCAATAGGAGTTTTCCTCCTATGGTTTTCAAAACAATC15140.10874921167595897No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC15070.1082464081873647No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC15020.1078872628383688No Hit
GGTTATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA14900.10702531400077864No Hit
TTAGGGGGCTATTAATGATATTAAAAATCCAACTATACCAAAGAATATCC14770.10609153609338927No Hit
CTAGGCGGGAAATCCATAAATAAAAATATATCAACAATAATTTTAAATAA14680.10544507446519666No Hit
TCCTATGGGCATGTACAATAAGCATGGAGTCGTATTGATGAGGACTTACC14500.10415215120881142No Hit
AAATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14460.1038648349296147No Hit
AGATAAGGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTG14400.10343386051081961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA302.5281042E-644.00000432
GGTCCGC302.5281042E-644.00000430
CCTACCG302.5281042E-644.00000414
GTAGCCG302.5281042E-644.00000443
ACCTACG302.5281042E-644.00000413
CAAGGCG650.044.0000041
TGTTACG600.044.0000041
GTCCGCA302.5281042E-644.00000431
CATACGT302.5281042E-644.00000424
CATACGA1450.044.00000418
CCCCAGT302.5281042E-644.00000418
ACCACTC302.5281042E-644.00000415
CGTAAGA302.5281042E-644.0000042
CGATGTT302.5281042E-644.00000410
CGGTCCG302.5281042E-644.00000429
ATACGTC302.5281042E-644.00000425
ATACGAA1450.044.00000419
GACGAAC650.044.00000420
TCGAATC302.5281042E-644.00000418
ACGAACA650.044.00000421
Produced by FastQC (version 0.11.5)