##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546223_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1392194
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93707701656522	33.0	31.0	34.0	30.0	34.0
2	32.3028112461338	33.0	31.0	34.0	30.0	34.0
3	32.17041374980786	33.0	31.0	34.0	30.0	34.0
4	35.942695486404915	37.0	35.0	37.0	35.0	37.0
5	35.922382225465704	37.0	35.0	37.0	35.0	37.0
6	35.988489391564684	37.0	35.0	37.0	35.0	37.0
7	36.226098517878974	37.0	35.0	37.0	35.0	37.0
8	36.136960797130286	37.0	36.0	37.0	35.0	37.0
9	37.90838489463394	39.0	39.0	39.0	35.0	39.0
10	37.58524674003767	39.0	37.0	39.0	35.0	39.0
11	37.36793363568583	39.0	37.0	39.0	35.0	39.0
12	36.67853546273005	39.0	35.0	39.0	33.0	39.0
13	36.403045839875766	39.0	35.0	39.0	33.0	39.0
14	37.556455493990065	40.0	35.0	41.0	33.0	41.0
15	37.83139706104178	40.0	35.0	41.0	33.0	41.0
16	37.930128272352846	40.0	35.0	41.0	34.0	41.0
17	37.80357047940158	40.0	35.0	41.0	34.0	41.0
18	37.60257047509183	39.0	36.0	41.0	34.0	41.0
19	37.36139359888062	38.0	35.0	41.0	34.0	41.0
20	37.057776430583665	37.0	35.0	41.0	34.0	41.0
21	36.896741402419494	37.0	35.0	41.0	33.0	41.0
22	36.82065286878122	37.0	35.0	41.0	33.0	41.0
23	36.76232479094149	37.0	35.0	41.0	33.0	41.0
24	36.73375621501027	37.0	35.0	41.0	33.0	41.0
25	36.72330221219169	37.0	35.0	41.0	33.0	41.0
26	36.66047404312905	37.0	35.0	41.0	33.0	41.0
27	36.54962311287076	37.0	35.0	41.0	33.0	41.0
28	36.491212431600765	37.0	35.0	41.0	33.0	41.0
29	36.39404493913923	37.0	35.0	41.0	32.0	41.0
30	36.17885869354415	36.0	35.0	40.0	32.0	41.0
31	35.790669260174944	36.0	35.0	40.0	31.0	41.0
32	35.32697382692355	36.0	35.0	41.0	29.0	41.0
33	34.78742545938282	36.0	35.0	40.0	24.0	41.0
34	34.186267143803235	36.0	34.0	40.0	20.0	41.0
35	33.898341754094616	36.0	34.0	40.0	18.0	41.0
36	33.7578857544279	36.0	34.0	40.0	17.0	41.0
37	33.67284085407637	36.0	34.0	40.0	16.0	41.0
38	33.57338560574173	36.0	33.0	40.0	16.0	41.0
39	33.47760369603662	36.0	33.0	40.0	15.0	41.0
40	33.33389599438009	36.0	33.0	40.0	15.0	41.0
41	33.290841649942465	36.0	33.0	40.0	12.0	41.0
42	33.270361745561324	36.0	33.0	40.0	12.0	41.0
43	33.177159936043395	35.0	33.0	40.0	10.0	41.0
44	33.0399534834944	35.0	33.0	40.0	10.0	41.0
45	32.95027919959431	35.0	33.0	40.0	10.0	41.0
46	32.724999533111045	35.0	33.0	40.0	10.0	41.0
47	32.673646776239515	35.0	33.0	40.0	10.0	41.0
48	32.775236066237895	35.0	33.0	40.0	10.0	41.0
49	32.82861799433125	36.0	33.0	40.0	10.0	41.0
50	32.73654533779057	35.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	3.0
14	10.0
15	16.0
16	63.0
17	170.0
18	525.0
19	1062.0
20	1839.0
21	3097.0
22	4997.0
23	7893.0
24	13127.0
25	22747.0
26	37287.0
27	49542.0
28	46969.0
29	35055.0
30	32344.0
31	34196.0
32	39834.0
33	51872.0
34	100354.0
35	226383.0
36	68411.0
37	82591.0
38	146698.0
39	385105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.292739373966562	24.179604279288665	28.09931661822993	22.42833972851485
2	36.60703896152404	24.13004222112723	28.93346760580781	10.32945121154092
3	23.01611700668154	23.662578634874162	42.21078384190709	11.110520516537207
4	22.57709773206895	25.77018720092171	39.54520706165951	12.107508005349828
5	17.84313105788417	29.510542352574426	41.1496529937638	11.4966735957776
6	17.81353748112691	38.75824777293969	34.9105081619372	8.517706583996196
7	77.74749783435354	3.40520071196974	17.346289382083242	1.5010120715934705
8	78.45034528233853	2.3378207347539206	17.22417996342464	1.9876540194829169
9	75.45801806357447	3.3736677503278996	18.844356461814947	2.323957724282679
10	45.71834097834066	22.95211730549047	22.93121504617891	8.398326669989958
11	34.87236692587384	20.86469270805649	28.358547731135168	15.9043926349345
12	30.825086158969224	17.951449295141337	36.211188957860756	15.01227558802868
13	12.22308097865671	35.60171930061472	41.91032284293712	10.264876877791457
14	7.836838831369766	35.05833238758391	42.03121116740914	15.07361761363718
15	7.120128372913545	17.46933257864924	62.45968593457521	12.950853113862005
16	14.806126157705032	14.016365535263045	56.23641532717423	14.941092979857693
17	13.621449309507152	19.30743847481026	35.02909795617565	32.042014259506935
18	11.731195508671924	21.309386479183217	50.74544208637589	16.213975925768967
19	17.397000705361464	18.412807410461475	47.05974885684036	17.13044302733671
20	18.785097479230625	17.527945099605372	41.961105995285145	21.725851425878865
21	12.665188903270666	21.92510526550179	46.792688375327	18.61701745590054
22	21.985944487621698	18.528452212838154	37.259103257160994	22.22650004237915
23	15.243780679991437	28.617060553342423	32.77854954122773	23.36060922543841
24	10.621149063995391	22.837621768230576	47.86035566882202	18.680873498952014
25	9.561454797248084	27.00844853518978	43.30689544704258	20.123201220519555
26	14.162537692304378	30.328819115726684	37.66824163873713	17.840401553231803
27	13.267116508187796	37.785969484137986	34.73610718046479	14.210806827209426
28	11.374636006188792	30.535686836748326	39.773048871062514	18.31662828600037
29	10.277949768494908	19.29975276434175	46.45616918331784	23.966128283845496
30	11.32414017011997	26.10720919641946	43.549821361103405	19.018829272357156
31	19.36389612367242	29.94668846439505	33.925516127781044	16.76389928415149
32	25.63249087411668	23.25193184283225	38.371807377420105	12.74376990563097
33	29.505227001409285	25.291661937919574	30.13804110634006	15.06506995433108
34	20.55654599861801	27.72257314713323	32.51450588064594	19.206374973602816
35	15.502365331268486	29.507094557224068	35.7694401785958	19.22109993291165
36	31.49604149996337	24.8334643016706	31.175683848659023	12.49481034970701
37	19.749977373843013	34.123189727868386	32.344630130570884	13.782202767717717
38	15.685026655767803	35.786176351859005	28.07194974263644	20.456847249736747
39	15.539357302215064	35.29192052257085	31.74837702216789	17.4203451530462
40	17.704069978752962	25.544643921752286	29.73658843523245	27.01469766426231
41	11.64744281328608	25.487898956610934	31.023262562545163	31.841395667557826
42	15.345346984687481	28.022028539126016	29.99395199232291	26.6386724838636
43	16.73150437367206	26.822411244409906	33.68883934279274	22.757245039125294
44	17.417400161184432	29.47060538976608	32.265618153791785	20.846376295257702
45	20.61400925445735	34.558761207130615	26.108645777815447	18.718583760596584
46	22.428842532003443	26.516203919855997	33.24342728096802	17.811526267172535
47	20.198334427529495	25.577828951999503	32.16714049909711	22.05669612137389
48	19.72749487499587	23.39386608475543	32.18323021073213	24.695408829516577
49	18.91417431765975	22.309318959857606	36.42344385911734	22.353062863365306
50	18.127502345219128	27.723075950621823	31.930679201318206	22.21874250284084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3494.0
1	4439.0
2	5384.0
3	18611.0
4	31838.0
5	23728.5
6	15619.0
7	18164.0
8	20709.0
9	23453.0
10	26197.0
11	26244.0
12	26291.0
13	24320.0
14	22349.0
15	20215.0
16	18081.0
17	16629.0
18	15177.0
19	14396.5
20	13616.0
21	13624.0
22	13632.0
23	11625.0
24	9618.0
25	12282.0
26	14946.0
27	15851.0
28	16756.0
29	18833.0
30	20910.0
31	31994.5
32	43079.0
33	44360.0
34	45641.0
35	45460.0
36	45279.0
37	50567.5
38	55856.0
39	62051.0
40	68246.0
41	88164.0
42	108082.0
43	124847.0
44	141612.0
45	150747.0
46	159882.0
47	160120.5
48	160359.0
49	138111.5
50	115864.0
51	89616.5
52	63369.0
53	47454.0
54	31539.0
55	25562.0
56	19585.0
57	17089.0
58	14593.0
59	13105.0
60	11617.0
61	11075.5
62	10534.0
63	9566.5
64	8599.0
65	6466.0
66	4333.0
67	3919.5
68	3506.0
69	3291.0
70	3076.0
71	2295.0
72	1514.0
73	1065.5
74	617.0
75	476.5
76	336.0
77	281.5
78	227.0
79	186.0
80	145.0
81	93.5
82	42.0
83	34.5
84	27.0
85	20.0
86	13.0
87	7.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1392194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.41884036630125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94294978201974	17.47390548841735
2	9.26706846314275	4.15513857077564
3	3.6403659226844667	2.4483834748675806
4	1.9517671931455	1.7502542854125132
5	1.156173159259016	1.2960030746615039
6	0.7918917532309179	1.0651976881844623
7	0.5363452310712418	0.841696668162919
8	0.4095618481825103	0.7345521355624804
9	0.3030632817366103	0.6114894600726404
>10	2.3620902500157652	11.606906369168222
>50	0.9075360548350543	15.010363383962359
>100	0.6518784112380821	26.071306430864045
>500	0.057063540449619025	8.705661778110061
>1k	0.021922716104938383	6.726941303059881
>5k	0.0	0.0
>10k+	3.223928838961527E-4	1.5021998887183747
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20784	1.4928953867061632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2850	0.20471284892766384	No Hit
TGTGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2084	0.1496917814614917	No Hit
TAAGTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	2060	0.14796788378631137	No Hit
GGGCAAGGGATAAAACTGGGTCACATAATAATCATATTCCAACTTTTGTA	1973	0.1417187547137827	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1892	0.13590060006004911	No Hit
AAGCTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1817	0.13051341982511058	No Hit
ACGAAAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT	1755	0.1260600174975614	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	1729	0.12419246168278272	No Hit
ATTATGGGGAGCTAAAATTTTTTTAAAATGGAATTTTGCTTCCACTATTT	1700	0.12210941865860647	No Hit
TGATTTGGGGATGAAGATTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGA	1691	0.12146295703041386	No Hit
TAGTTAGGGCTATTAATGATATTAAAATCCCAACTATCCAAAGAATATCC	1669	0.1198827174948319	No Hit
GGGGAGGGGGGCATAAGTAAATAGAATTAAGTAAATTACCTTACTATTTT	1635	0.11744052912165975	No Hit
AGCATAGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA	1573	0.11298712679411059	No Hit
GGATTTGGGATCCCAATAGGAGTTTTCCTCCTATGGTTTTCAAAACAATC	1514	0.10874921167595897	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	1507	0.1082464081873647	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	1502	0.1078872628383688	No Hit
GGTTATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	1490	0.10702531400077864	No Hit
TTAGGGGGCTATTAATGATATTAAAAATCCAACTATACCAAAGAATATCC	1477	0.10609153609338927	No Hit
CTAGGCGGGAAATCCATAAATAAAAATATATCAACAATAATTTTAAATAA	1468	0.10544507446519666	No Hit
TCCTATGGGCATGTACAATAAGCATGGAGTCGTATTGATGAGGACTTACC	1450	0.10415215120881142	No Hit
AAATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1446	0.1038648349296147	No Hit
AGATAAGGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTG	1440	0.10343386051081961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06529262444745489	0.0
2	0.0	0.0	0.0	0.2737405850046761	0.0
3	0.0	0.0	0.0	0.4428262153119465	0.0
4	0.0	0.0	0.0	0.7822903991828725	0.0
5	0.0	0.0	0.0	1.4361504215648107	0.0
6	0.0	0.0	0.0	2.054598712535753	0.0
7	0.0	0.0	0.0	2.4288999952592816	0.0
8	0.0	0.0	0.0	3.28862213168567	0.0
9	0.0	0.0	0.0	3.682317263254977	0.0
10	0.0	0.0	0.0	4.325546583306637	0.0
11	0.0	0.0	0.0	5.067612703401968	0.0
12	0.0	0.0	0.0	5.661351794361993	0.0
13	0.0	0.0	0.0	5.8898400653931855	0.0
14	0.0	0.0	0.0	5.954342570072849	0.0
15	0.0	0.0	0.0	6.056986310815877	0.0
16	0.0	0.0	0.0	6.324836912097021	0.0
17	0.0	0.0	0.0	6.640094699445624	0.0
18	0.0	0.0	0.0	7.191167322944934	0.0
19	0.0	0.0	0.0	7.392576034661836	0.0
20	0.0	0.0	0.0	7.693539837120401	0.0
21	0.0	0.0	0.0	8.107921740791872	0.0
22	0.0	0.0	0.0	8.387193164171086	0.0
23	0.0	0.0	0.0	8.726513689902413	0.0
24	0.0	0.0	0.0	8.977053485361953	0.0
25	0.0	0.0	0.0	9.220913177330171	0.0
26	0.0	0.0	0.0	9.482586478608585	0.0
27	0.0	0.0	0.0	9.652749544962843	0.0
28	0.0	0.0	0.0	9.826647722946658	0.0
29	0.0	0.0	0.0	10.05334026723287	0.0
30	0.0	0.0	0.0	10.405087222039457	0.0
31	0.0	0.0	0.0	10.778598384995195	0.0
32	0.0	0.0	0.0	11.07963401652356	0.0
33	0.0	0.0	0.0	11.32320639221258	0.0
34	0.0	0.0	0.0	11.552341124871965	0.0
35	0.0	0.0	0.0	12.010610590190735	0.0
36	0.0	0.0	0.0	12.33520615661323	0.0
37	0.0	0.0	0.0	12.722221184691215	0.0
38	0.0	0.0	0.0	13.041357741808971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	30	2.5281042E-6	44.000004	32
GGTCCGC	30	2.5281042E-6	44.000004	30
CCTACCG	30	2.5281042E-6	44.000004	14
GTAGCCG	30	2.5281042E-6	44.000004	43
ACCTACG	30	2.5281042E-6	44.000004	13
CAAGGCG	65	0.0	44.000004	1
TGTTACG	60	0.0	44.000004	1
GTCCGCA	30	2.5281042E-6	44.000004	31
CATACGT	30	2.5281042E-6	44.000004	24
CATACGA	145	0.0	44.000004	18
CCCCAGT	30	2.5281042E-6	44.000004	18
ACCACTC	30	2.5281042E-6	44.000004	15
CGTAAGA	30	2.5281042E-6	44.000004	2
CGATGTT	30	2.5281042E-6	44.000004	10
CGGTCCG	30	2.5281042E-6	44.000004	29
ATACGTC	30	2.5281042E-6	44.000004	25
ATACGAA	145	0.0	44.000004	19
GACGAAC	65	0.0	44.000004	20
TCGAATC	30	2.5281042E-6	44.000004	18
ACGAACA	65	0.0	44.000004	21
>>END_MODULE