FastQCFastQC Report
Thu 26 May 2016
SRR1546222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546222_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1422648
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214211.505713289583931No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30220.2124207815285299No Hit
GGGCAAGGGATAAAACTGGGTCACATAATAATCATATTCCAACTTTTGTA21600.15182954602965737No Hit
TGTGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT20770.14599535514055478No Hit
TAAGTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA20550.14444894309766013No Hit
AAGCTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18160.12764928499530453No Hit
ACGAAAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT17870.1256108327569434No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17790.1250485011049817No Hit
ATTATGGGGAGCTAAAATTTTTTTAAAATGGAATTTTGCTTCCACTATTT17500.12301004886662056No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT16390.11520769719565206No Hit
TAGTTAGGGCTATTAATGATATTAAAATCCCAACTATCCAAAGAATATCC16230.11408303389172866No Hit
GGGGAGGGGGGCATAAGTAAATAGAATTAAGTAAATTACCTTACTATTTT16110.11323953641378612No Hit
TGATTTGGGGATGAAGATTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGA16040.11274749621831964No Hit
GGATTTGGGATCCCAATAGGAGTTTTCCTCCTATGGTTTTCAAAACAATC15860.11148225000140582No Hit
CTAGGCGGGAAATCCATAAATAAAAATATATCAACAATAATTTTAAATAA15590.10958438067603513No Hit
AGCATAGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA15240.1071241796987027No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC14840.10431252143889423No Hit
GTAGAAGGGGATTTTAGAGAACCTACTTTTAAATTACACTTTGATACAGT14720.10346902396095169No Hit
AGATAAGGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTG14690.10325814959146605No Hit
TTAGGGGGCTATTAATGATATTAAAAATCCAACTATACCAAAGAATATCC14520.10206319483104745No Hit
AGGCGAGGGCCATCATGCTATTAATGATATTAAAATCCCAACTATACCAA14510.10199290337455223No Hit
AAATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14400.1012196973531049No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC14360.10093853152712408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA551.8189894E-1244.09
CTTAACG302.5281424E-644.01
ATCCTAC502.7284841E-1144.032
CTTCGAA502.7284841E-1144.024
TATCACG254.4433742E-544.01
GAGTGCA750.044.09
TAGAGCG254.4433742E-544.01
GTACCTA800.044.09
TCGCAAA254.4433742E-544.011
GCCCACG207.857114E-444.010
TCCGCAA302.5281424E-644.032
CTCGTAG254.4433742E-544.01
GTATTAC351.4464058E-744.034
TACCAAC302.5281424E-644.014
CACGGGA1100.044.04
ACGTTAC254.4433742E-544.011
CCTAGAA207.857114E-444.025
GGCACGG254.4433742E-544.02
CCGGCAC302.5281424E-644.021
CAGTTCG351.4464058E-744.01
Produced by FastQC (version 0.11.5)