##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546222_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1422648
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875185569445147	31.0	31.0	34.0	30.0	34.0
2	32.27927217414287	33.0	31.0	34.0	30.0	34.0
3	32.27404319269419	34.0	31.0	34.0	30.0	34.0
4	35.98385124078479	37.0	35.0	37.0	35.0	37.0
5	35.8862192193712	37.0	35.0	37.0	35.0	37.0
6	35.97618525453942	37.0	35.0	37.0	35.0	37.0
7	36.20536914261293	37.0	35.0	37.0	35.0	37.0
8	36.12328207680326	37.0	36.0	37.0	35.0	37.0
9	37.89050629530284	39.0	38.0	39.0	35.0	39.0
10	37.59175706147972	39.0	37.0	39.0	35.0	39.0
11	37.37036076387132	39.0	37.0	39.0	35.0	39.0
12	36.66991202321305	39.0	35.0	39.0	33.0	39.0
13	36.3412200347521	39.0	35.0	39.0	33.0	39.0
14	37.490919046735385	40.0	35.0	41.0	33.0	41.0
15	37.80009320647131	40.0	35.0	41.0	33.0	41.0
16	37.896198497449824	40.0	35.0	41.0	34.0	41.0
17	37.78246762375514	40.0	35.0	41.0	34.0	41.0
18	37.58726332866598	39.0	36.0	41.0	34.0	41.0
19	37.34864773295995	38.0	35.0	41.0	34.0	41.0
20	37.04166736958123	37.0	35.0	41.0	34.0	41.0
21	36.883000573578286	37.0	35.0	41.0	33.0	41.0
22	36.81021025580467	37.0	35.0	41.0	33.0	41.0
23	36.74382700429059	37.0	35.0	41.0	33.0	41.0
24	36.722187076493974	37.0	35.0	41.0	33.0	41.0
25	36.70049653884868	37.0	35.0	41.0	33.0	41.0
26	36.656212218342134	37.0	35.0	41.0	33.0	41.0
27	36.54042110205757	37.0	35.0	41.0	33.0	41.0
28	36.47561027042529	37.0	35.0	41.0	33.0	41.0
29	36.37721066630677	37.0	35.0	41.0	32.0	41.0
30	36.168359987853634	36.0	35.0	40.0	32.0	41.0
31	35.78935056317515	36.0	35.0	40.0	31.0	41.0
32	35.32853102102558	36.0	35.0	40.0	29.0	41.0
33	34.78132538758709	36.0	35.0	40.0	24.0	41.0
34	34.310002193093446	36.0	34.0	40.0	21.0	41.0
35	33.98072397388532	36.0	34.0	40.0	18.0	41.0
36	33.79811098739815	36.0	34.0	40.0	17.0	41.0
37	33.62902629462805	36.0	33.0	40.0	16.0	41.0
38	33.53782031816725	36.0	33.0	40.0	15.0	41.0
39	33.455365628040106	36.0	33.0	40.0	15.0	41.0
40	33.32843753338844	36.0	33.0	40.0	15.0	41.0
41	33.31276464733371	36.0	33.0	40.0	13.0	41.0
42	33.27131236960935	36.0	33.0	40.0	12.0	41.0
43	33.200586511912995	35.0	33.0	40.0	10.0	41.0
44	33.057577840758924	35.0	33.0	40.0	10.0	41.0
45	32.962462956402426	35.0	33.0	40.0	10.0	41.0
46	32.75144870691837	35.0	33.0	40.0	10.0	41.0
47	32.68066802188595	35.0	33.0	40.0	10.0	41.0
48	32.791984384050025	35.0	33.0	40.0	10.0	41.0
49	32.84301949603837	36.0	33.0	40.0	10.0	41.0
50	32.73205107658395	35.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	6.0
14	14.0
15	20.0
16	72.0
17	203.0
18	517.0
19	1046.0
20	1976.0
21	3211.0
22	5171.0
23	8128.0
24	13235.0
25	23020.0
26	37323.0
27	50323.0
28	48450.0
29	36185.0
30	33475.0
31	34806.0
32	41049.0
33	54101.0
34	105175.0
35	227202.0
36	70855.0
37	84576.0
38	150008.0
39	392498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.397779352306404	24.27859878198964	28.067308287081556	22.2563135786224
2	36.64370947697533	24.06856790998195	28.95059072940039	10.337131883642336
3	23.031628343764584	23.690329582581214	42.20390426865957	11.07413780499463
4	22.664214900664113	25.830282684121443	39.404195556455285	12.101306858759159
5	17.913496521978733	29.635651264402718	40.91560245401533	11.53524975960322
6	17.89908677339721	38.63415265055024	34.86442183871203	8.60233873734051
7	77.78290905410192	3.3891728663731295	17.308146498641968	1.519771580882973
8	78.48013001107793	2.331427029033183	17.18717490201371	2.0012680578751736
9	75.44297675883283	3.374903700704601	18.83072973778475	2.3513898026778235
10	45.765361494902464	22.888233772514354	22.92366066658794	8.422744065995243
11	34.85914997947489	20.83073254944301	28.390227238220554	15.919890232861537
12	30.884097823214173	17.922564119866617	36.108650910133775	15.08468714678543
13	12.310072484549938	35.50618283651332	41.93201691493609	10.251727764000652
14	7.883046263025006	35.03621415838633	42.02634804955267	15.054391529035996
15	7.1650893263829145	17.498776928656984	62.300583137923084	13.035550607037019
16	14.764439270993247	14.03579803296388	56.234430442386305	14.96533225365656
17	13.637667223375002	19.256977129971713	35.094485775820864	32.01086987083242
18	11.724966400683796	21.2844639011196	50.68871569074008	16.30185400745652
19	17.440786477048434	18.3954850391664	47.04691532972316	17.116813154062
20	18.799520331100876	17.498425471374507	41.95809504529581	21.7439591522288
21	12.716216520179271	21.913642728208245	46.75056654913935	18.619574202473135
22	21.887916055130997	18.52362636435717	37.26740557045735	22.32105201005449
23	15.302590661920586	28.639410451496083	32.69185350135803	23.3661453852253
24	10.628349387901997	22.845355984052272	47.78286687922803	18.743427748817698
25	9.603570243658305	27.055111313550505	43.16591314225304	20.175405300538152
26	14.09308557000748	30.24571081532466	37.759234891554335	17.90196872311352
27	13.283609157008621	37.70019006809836	34.716880071528585	14.29932070336443
28	11.451954383656393	30.50164200842373	39.80963667751966	18.23676693040021
29	10.29741721072254	19.31489729012377	46.399249849576286	23.988435649577408
30	11.369572796644006	26.086705917415976	43.51413701773031	19.029584268209703
31	19.391163520421074	29.86831598540187	33.897422271707406	16.84309822246965
32	25.61835394278838	23.25262468298553	38.299987066372005	12.829034307854087
33	29.394973317363117	25.250237585122953	30.153839881685418	15.20094921582851
34	20.585837115013693	27.686047427051523	32.45201905179636	19.276096406138414
35	15.525766036292884	29.479955688265825	35.67860777929607	19.315670496145216
36	31.4577463996716	24.91396325724986	30.958887932925084	12.66940241015346
37	19.75351597865389	34.049392400650056	32.314388379978745	13.882703240717309
38	15.750417531251582	35.71579195978204	28.081647744206577	20.452142764759802
39	15.597393030461507	35.179257272354086	31.752127019473548	17.47122267771086
40	17.836246211290497	25.45598067828444	29.704747766137512	27.00302534428755
41	11.768687686623817	25.41134560340998	31.008654284123686	31.811312425842512
42	15.366766761700715	27.967213253032376	29.92911809527023	26.736901889996684
43	16.729858686055863	26.78976106528108	33.67867525909431	22.801704989568748
44	17.437763944419142	29.344855508882027	32.32113635980228	20.89624418689655
45	20.67834067176139	34.40830057751461	26.11946173614274	18.79389701458126
46	22.487783344861132	26.538820565593173	33.0710056176932	17.902390471852488
47	20.169289943823067	25.56577593332996	32.21323897408214	22.05169514876484
48	19.77361933521152	23.307873767790767	32.22223628051352	24.69627061648419
49	18.972156148253116	22.223065719700163	36.3740714498597	22.430706682187022
50	18.112702509686162	27.63663253313539	31.990907097187783	22.259757859990664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3626.0
1	4506.5
2	5387.0
3	18962.5
4	32538.0
5	24176.0
6	15814.0
7	18280.5
8	20747.0
9	23568.0
10	26389.0
11	26467.0
12	26545.0
13	24717.5
14	22890.0
15	20556.0
16	18222.0
17	16895.0
18	15568.0
19	14613.5
20	13659.0
21	13756.0
22	13853.0
23	11970.5
24	10088.0
25	12783.5
26	15479.0
27	16378.0
28	17277.0
29	19248.0
30	21219.0
31	32377.5
32	43536.0
33	44760.0
34	45984.0
35	45964.0
36	45944.0
37	51226.5
38	56509.0
39	63000.0
40	69491.0
41	89921.5
42	110352.0
43	127326.0
44	144300.0
45	154113.5
46	163927.0
47	164848.5
48	165770.0
49	142265.5
50	118761.0
51	92206.5
52	65652.0
53	49064.0
54	32476.0
55	26281.5
56	20087.0
57	17548.5
58	15010.0
59	13386.5
60	11763.0
61	11294.0
62	10825.0
63	9742.0
64	8659.0
65	6635.5
66	4612.0
67	4076.0
68	3540.0
69	3371.5
70	3203.0
71	2369.0
72	1535.0
73	1104.5
74	674.0
75	514.5
76	355.0
77	258.0
78	161.0
79	154.5
80	148.0
81	89.5
82	31.0
83	26.5
84	22.0
85	16.0
86	10.0
87	7.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1422648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.41430625862092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75992787233854	17.42934837978869
2	9.169230307399213	4.110438725317495
3	3.7596040755281974	2.528067514801451
4	1.9707701474765842	1.7669378260355066
5	1.2501273921606926	1.4010369115090424
6	0.8050397918361214	1.0826645066754752
7	0.5749053883325072	0.9020273810671341
8	0.4156662351800646	0.7453496237355133
9	0.3056432396927856	0.6165703062316061
>10	2.35592675495961	11.482340264181055
>50	0.8934841584783446	14.689595964552952
>100	0.6607170537162941	26.35852871823002
>500	0.0574811203519765	8.833393954990223
>1k	0.02116063221748585	6.5372821035856985
>5k	0.0	0.0
>10k+	3.1583033160426647E-4	1.516417819298175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21421	1.505713289583931	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3022	0.2124207815285299	No Hit
GGGCAAGGGATAAAACTGGGTCACATAATAATCATATTCCAACTTTTGTA	2160	0.15182954602965737	No Hit
TGTGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2077	0.14599535514055478	No Hit
TAAGTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	2055	0.14444894309766013	No Hit
AAGCTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1816	0.12764928499530453	No Hit
ACGAAAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT	1787	0.1256108327569434	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1779	0.1250485011049817	No Hit
ATTATGGGGAGCTAAAATTTTTTTAAAATGGAATTTTGCTTCCACTATTT	1750	0.12301004886662056	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	1639	0.11520769719565206	No Hit
TAGTTAGGGCTATTAATGATATTAAAATCCCAACTATCCAAAGAATATCC	1623	0.11408303389172866	No Hit
GGGGAGGGGGGCATAAGTAAATAGAATTAAGTAAATTACCTTACTATTTT	1611	0.11323953641378612	No Hit
TGATTTGGGGATGAAGATTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGA	1604	0.11274749621831964	No Hit
GGATTTGGGATCCCAATAGGAGTTTTCCTCCTATGGTTTTCAAAACAATC	1586	0.11148225000140582	No Hit
CTAGGCGGGAAATCCATAAATAAAAATATATCAACAATAATTTTAAATAA	1559	0.10958438067603513	No Hit
AGCATAGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA	1524	0.1071241796987027	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	1484	0.10431252143889423	No Hit
GTAGAAGGGGATTTTAGAGAACCTACTTTTAAATTACACTTTGATACAGT	1472	0.10346902396095169	No Hit
AGATAAGGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTG	1469	0.10325814959146605	No Hit
TTAGGGGGCTATTAATGATATTAAAAATCCAACTATACCAAAGAATATCC	1452	0.10206319483104745	No Hit
AGGCGAGGGCCATCATGCTATTAATGATATTAAAATCCCAACTATACCAA	1451	0.10199290337455223	No Hit
AAATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1440	0.1012196973531049	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	1436	0.10093853152712408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06508988871456607	0.0
2	0.0	0.0	0.0	0.2777918360690768	0.0
3	0.0	0.0	0.0	0.45373135167659184	0.0
4	0.0	0.0	0.0	0.7878266443983333	0.0
5	0.0	0.0	0.0	1.4544005263424262	0.0
6	0.0	0.0	0.0	2.0988325994905277	0.0
7	0.0	0.0	0.0	2.4765788866957954	0.0
8	0.0	0.0	0.0	3.355925007450894	0.0
9	0.0	0.0	0.0	3.7593979677334097	0.0
10	0.0	0.0	0.0	4.425901558221008	0.0
11	0.0	0.0	0.0	5.191516102366854	0.0
12	0.0	0.0	0.0	5.808956256220794	0.0
13	0.0	0.0	0.0	6.049634203260399	0.0
14	0.0	0.0	0.0	6.114724091974965	0.0
15	0.0	0.0	0.0	6.225292553041933	0.0
16	0.0	0.0	0.0	6.508145373978665	0.0
17	0.0	0.0	0.0	6.832329571334582	0.0
18	0.0	0.0	0.0	7.386437122886336	0.0
19	0.0	0.0	0.0	7.606449381716349	0.0
20	0.0	0.0	0.0	7.9046257401690365	0.0
21	0.0	0.0	0.0	8.34542346385051	0.0
22	0.0	0.0	0.0	8.631931440524992	0.0
23	0.0	0.0	0.0	8.990488160107068	0.0
24	0.0	0.0	0.0	9.24353740348983	0.0
25	0.0	0.0	0.0	9.494477903177737	0.0
26	0.0	0.0	0.0	9.777963347222926	0.0
27	0.0	0.0	0.0	9.958260933133143	0.0
28	0.0	0.0	0.0	10.138558519043361	0.0
29	0.0	0.0	0.0	10.380642295212871	0.0
30	0.0	0.0	0.0	10.745665828792506	0.0
31	0.0	0.0	0.0	11.146116256445726	0.0
32	0.0	0.0	0.0	11.471987448757528	0.0
33	0.0	0.0	0.0	11.735369536245086	0.0
34	0.0	0.0	0.0	11.981811382717298	0.0
35	0.0	0.0	0.0	12.448968402584477	0.0
36	0.0	0.0	0.0	12.791779835911624	0.0
37	0.0	0.0	0.0	13.197220956976006	0.0
38	0.0	0.0	0.0	13.525130601526168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCTA	55	1.8189894E-12	44.0	9
CTTAACG	30	2.5281424E-6	44.0	1
ATCCTAC	50	2.7284841E-11	44.0	32
CTTCGAA	50	2.7284841E-11	44.0	24
TATCACG	25	4.4433742E-5	44.0	1
GAGTGCA	75	0.0	44.0	9
TAGAGCG	25	4.4433742E-5	44.0	1
GTACCTA	80	0.0	44.0	9
TCGCAAA	25	4.4433742E-5	44.0	11
GCCCACG	20	7.857114E-4	44.0	10
TCCGCAA	30	2.5281424E-6	44.0	32
CTCGTAG	25	4.4433742E-5	44.0	1
GTATTAC	35	1.4464058E-7	44.0	34
TACCAAC	30	2.5281424E-6	44.0	14
CACGGGA	110	0.0	44.0	4
ACGTTAC	25	4.4433742E-5	44.0	11
CCTAGAA	20	7.857114E-4	44.0	25
GGCACGG	25	4.4433742E-5	44.0	2
CCGGCAC	30	2.5281424E-6	44.0	21
CAGTTCG	35	1.4464058E-7	44.0	1
>>END_MODULE