FastQCFastQC Report
Thu 26 May 2016
SRR1546221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546221_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1233866
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219011.7749901529015306No Hit
TTCAGAGGGAACGGCATTAGGAGAATTGTTTTAGATATATTGAAGCCGCA23820.19305175764629223No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC22490.18227262928065124No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG17130.13883193150633863No Hit
TTAGAAGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT15660.1269181580495775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15560.12610769727020602No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT15160.12286585415271999No Hit
AGTTAAGGGCAGCCTAACACTTCTATGACAAACCGACGAAAATTATAAAA14870.12051551789254263No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC14310.11597693752806221No Hit
AGCTAAGGGGAGGCCAGGAGGATAATTAACTTGAGGAATAGACAACTTAC13770.11160044931945608No Hit
ACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTTTAGCAA13490.10933115913721587No Hit
TTATGAGGGAGGATTATGGGTTTTTGTTTTGTTTTCTCTTAATTTTGTTA13010.10544094739623265No Hit
TCCTGTGGGTTTTTTTTTTCATTTCATCTGTACATTAATTCTGTTGGGTA12350.10009190625238074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC207.8567065E-444.00000427
AGTCACG207.8567065E-444.0000041
CAACGGC207.8567065E-444.00000434
TAGCGCA207.8567065E-444.00000424
GTAATAC207.8567065E-444.00000424
GTATCCG207.8567065E-444.00000428
ACGTATG207.8567065E-444.0000041
TCGACTA207.8567065E-444.00000423
TGGTCAT1600.044.00000434
CCTTCGT302.5278696E-644.023
ACGCCAA254.4430304E-544.04
TAGGTTC254.4430304E-544.044
GACCCTA302.5278696E-644.043
TCGATAG302.5278696E-644.01
CGTTACC454.802132E-1044.044
ATTTTCG454.802132E-1044.044
TACGATC254.4430304E-544.018
CGGATGA302.5278696E-644.011
GTGTAAC1200.044.010
TTACGAT254.4430304E-544.017
Produced by FastQC (version 0.11.5)