FastQCFastQC Report
Thu 26 May 2016
SRR1546220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546220_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1263920
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224001.7722640673460344No Hit
TTCAGAGGGAACGGCATTAGGAGAATTGTTTTAGATATATTGAAGCCGCA25580.20238622697639091No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC21420.16947275143996454No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG17270.13663839483511614No Hit
TTAGAAGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT16260.1286473827457434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16100.12738147984049625No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC15140.11978606240901322No Hit
AGTTAAGGGCAGCCTAACACTTCTATGACAAACCGACGAAAATTATAAAA14990.11859927843534401No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT14690.11622571048800556No Hit
ACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTTTAGCAA13850.10957972023545794No Hit
AGCTAAGGGGAGGCCAGGAGGATAATTAACTTGAGGAATAGACAACTTAC13800.1091841255775682No Hit
TTATGAGGGAGGATTATGGGTTTTTGTTTTGTTTTCTCTTAATTTTGTTA13140.10396227609342365No Hit
TCCTGTGGGTTTTTTTTTTCATTTCATCTGTACATTAATTCTGTTGGGTA12970.10261725425659851No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACC207.856779E-444.00000425
CGTGGAC207.856779E-444.00000425
ATCTCGA207.856779E-444.0000042
ATCTCCG207.856779E-444.0000041
GTCGATC408.312782E-944.00000429
CGACGGC302.5279169E-644.00000419
CGACCGA207.856779E-444.00000425
CGTTATC302.5279169E-644.00000417
CGTTACC207.856779E-444.00000444
CGGATGA207.856779E-444.00000411
CATACCG207.856779E-444.0000041
CAGTACG302.5279169E-644.0000041
ATCGATT950.044.00000414
AACCGAA700.044.010
TCGTTAG551.8189894E-1244.01
CGAACTG750.044.036
ATAGACC254.4430926E-544.038
AAGCGGT254.4430926E-544.016
TACGGGT750.044.04
TCGATAG750.044.01
Produced by FastQC (version 0.11.5)