##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546220_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1263920
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807676118741693	31.0	31.0	34.0	30.0	34.0
2	32.19551474776885	33.0	31.0	34.0	30.0	34.0
3	32.2219365149693	33.0	31.0	34.0	30.0	34.0
4	35.93733226786505	37.0	35.0	37.0	35.0	37.0
5	35.854722609025885	37.0	35.0	37.0	35.0	37.0
6	35.94438334704728	37.0	35.0	37.0	35.0	37.0
7	36.14958699917716	37.0	35.0	37.0	35.0	37.0
8	36.03496582062156	37.0	35.0	37.0	35.0	37.0
9	37.78134612950187	39.0	38.0	39.0	35.0	39.0
10	37.47657842268498	39.0	37.0	39.0	35.0	39.0
11	37.303318247990376	39.0	37.0	39.0	34.0	39.0
12	36.74945645294006	39.0	35.0	39.0	33.0	39.0
13	36.490489113235014	39.0	35.0	39.0	33.0	39.0
14	37.58049322741946	40.0	35.0	41.0	33.0	41.0
15	37.81031157035255	40.0	35.0	41.0	33.0	41.0
16	37.91047376416229	40.0	35.0	41.0	33.0	41.0
17	37.825543547059944	40.0	35.0	41.0	33.0	41.0
18	37.65348281536806	39.0	36.0	41.0	33.0	41.0
19	37.39926894107222	38.0	36.0	41.0	33.0	41.0
20	37.07104088866384	38.0	35.0	41.0	33.0	41.0
21	36.973346414330024	38.0	35.0	41.0	33.0	41.0
22	36.901884612950184	38.0	35.0	41.0	33.0	41.0
23	36.85002057092221	38.0	35.0	41.0	33.0	41.0
24	36.792682290018355	38.0	35.0	41.0	33.0	41.0
25	36.77533467308057	38.0	35.0	41.0	33.0	41.0
26	36.71909931008292	38.0	35.0	41.0	33.0	41.0
27	36.62718210013292	38.0	35.0	41.0	32.0	41.0
28	36.560646243433126	38.0	35.0	41.0	32.0	41.0
29	36.45084815494651	38.0	35.0	41.0	32.0	41.0
30	36.22260427875182	38.0	35.0	41.0	31.0	41.0
31	35.90100322805241	38.0	35.0	41.0	30.0	41.0
32	35.495976011139945	38.0	35.0	41.0	29.0	41.0
33	35.01157984682575	38.0	35.0	41.0	24.0	41.0
34	34.583462560921575	38.0	34.0	41.0	21.0	41.0
35	34.26863409076524	38.0	34.0	41.0	18.0	41.0
36	34.06053072979302	37.0	34.0	40.0	18.0	41.0
37	33.93021551996962	37.0	34.0	40.0	18.0	41.0
38	33.89348613836319	37.0	33.0	40.0	18.0	41.0
39	33.84004604721818	37.0	34.0	40.0	17.0	41.0
40	33.701535698461925	37.0	33.0	40.0	15.0	41.0
41	33.606785239572126	37.0	33.0	40.0	15.0	41.0
42	33.51068896765618	37.0	33.0	40.0	15.0	41.0
43	33.37909282233053	36.0	33.0	40.0	14.0	41.0
44	33.23809576555478	36.0	33.0	40.0	12.0	41.0
45	33.16921007658713	36.0	33.0	40.0	13.0	41.0
46	33.092346825748464	36.0	33.0	40.0	12.0	41.0
47	33.03815273118552	35.0	33.0	40.0	12.0	41.0
48	32.99181672890689	35.0	33.0	40.0	12.0	41.0
49	32.9292020064561	36.0	33.0	40.0	12.0	41.0
50	32.728401322868535	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	0.0
12	6.0
13	3.0
14	16.0
15	28.0
16	99.0
17	219.0
18	566.0
19	1181.0
20	2262.0
21	3405.0
22	5353.0
23	8142.0
24	12602.0
25	20106.0
26	31426.0
27	39305.0
28	37929.0
29	33088.0
30	31270.0
31	33079.0
32	37901.0
33	48897.0
34	88928.0
35	163150.0
36	71840.0
37	89473.0
38	149575.0
39	354065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.844705361098804	22.756107981517818	26.5607000443066	21.83848661307678
2	36.68998037850496	23.075748465092726	29.157620735489587	11.076650420912717
3	25.491882397620103	23.470868409393	38.73900246851066	12.298246724476233
4	22.62255522501424	25.9769605671245	39.73210329767707	11.66838091018419
5	19.66493132476739	29.462624216722578	38.21594721184885	12.65649724666118
6	17.21667510601937	39.42852395721248	35.537217545414265	7.817583391353883
7	77.29674346477626	3.5950060130388	17.584578137856827	1.523672384328122
8	77.279732894487	3.6512595733907207	16.62201721627951	2.4469903158427746
9	73.18445787708083	4.615323754668017	18.58226786505475	3.617950503196405
10	40.344642065953536	25.63508766377619	24.23515728843598	9.785112981834294
11	33.613994556617506	20.58729982910311	32.79210709538579	13.0065985188936
12	32.14807899234129	20.41529527185265	34.937337806190264	12.499287929615798
13	18.39950313310969	33.05106335844041	35.65581682384961	12.893616684600293
14	12.876131400721565	34.309845559845556	38.445629470219636	14.368393569213241
15	11.581666561174758	21.692433065383884	51.625814924995254	15.100085448446105
16	13.04908538515096	20.94111969111969	47.835701626685236	18.174093297044116
17	14.02802392556491	19.815336413697068	38.399424014178116	27.757215646559906
18	15.965092727387809	23.05027216912463	43.6850433571745	17.29959174631306
19	19.432005190201913	24.56476675738971	36.35143047028293	19.651797582125454
20	21.775033229951262	21.944980694980696	39.46665928223305	16.81332679283499
21	17.96260839293626	25.461738084688907	40.803769225900375	15.771884296474461
22	18.529258180897525	21.4767548579024	36.571064624343315	23.422922336856765
23	15.05744034432559	26.476042787518196	36.20830432305842	22.25821254509779
24	13.80324704095196	25.452797645420596	46.00125007911893	14.742705234508513
25	14.510649408190393	26.767200455725042	41.21091524780049	17.51123488828407
26	14.078976517501108	28.635752262801446	37.02441610228496	20.260855117412493
27	13.995664282549528	31.99316412431166	36.25696246597886	17.754209127159946
28	12.756740932970443	28.252025444648392	41.1548990442433	17.83633457813786
29	14.167035888347362	24.0592759035382	39.82649218304956	21.947196025064876
30	15.333090701943162	30.871969744920563	37.68197354262928	16.112966010506995
31	20.266551680486106	29.282707766314324	34.2975030065194	16.15323754668017
32	21.294069244888917	26.583328058737894	34.92997974555352	17.192622950819672
33	20.398917653016014	29.990901322868535	30.803848344831952	18.8063326792835
34	16.204585733274257	27.686641559592378	32.4361510222166	23.672621684916766
35	17.795509209443637	26.80715551617191	34.65646559908855	20.740869675295905
36	25.438872713462878	28.13390087980252	29.819055003481232	16.608171403253372
37	15.660563959744286	32.020143679979746	35.044623077409966	17.274669282866004
38	15.916038989809481	29.219570858915123	33.141812772960314	21.722577378315084
39	16.11391543768593	31.052360908918285	32.491376036458	20.34234761693778
40	18.510269637318817	26.236233305905436	33.91733654028736	21.336160516488388
41	15.16931451357681	26.95415849104374	34.30177542882461	23.574751566554845
42	17.837204886385212	27.026631432369136	31.938018229001834	23.198145452243814
43	16.907161845686435	26.884138236597256	32.57911893157795	23.62958098613836
44	15.618947401734287	28.878093550224698	33.30202860940566	22.200930438635357
45	16.367412494461675	34.33405595290841	29.155326286473827	20.143205266156087
46	19.07581176023799	30.85029115766821	32.06539970884233	18.008497373251473
47	17.156228242293817	29.992641939363253	32.82383378694854	20.027296031394393
48	16.987784036964364	28.809497436546614	34.0506519399962	20.15206658649282
49	19.177400468384075	25.815399708842328	34.715646559908855	20.29155326286474
50	18.552440027849865	29.566665611747577	32.09237926451041	19.788515095892144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2990.0
1	5065.5
2	7141.0
3	23001.0
4	38861.0
5	27506.5
6	16152.0
7	17135.0
8	18118.0
9	19359.5
10	20601.0
11	19867.0
12	19133.0
13	18087.5
14	17042.0
15	15607.5
16	14173.0
17	13154.0
18	12135.0
19	11772.0
20	11409.0
21	10715.5
22	10022.0
23	9426.0
24	8830.0
25	11972.0
26	15114.0
27	19132.5
28	23151.0
29	27826.5
30	32502.0
31	37718.0
32	42934.0
33	50833.5
34	58733.0
35	64747.0
36	70761.0
37	70049.5
38	69338.0
39	73715.5
40	78093.0
41	84160.5
42	90228.0
43	96901.5
44	103575.0
45	96119.0
46	88663.0
47	82775.0
48	76887.0
49	75026.0
50	73165.0
51	62172.5
52	51180.0
53	44566.0
54	37952.0
55	33670.0
56	29388.0
57	27957.0
58	26526.0
59	25168.0
60	23810.0
61	22265.5
62	20721.0
63	18818.0
64	16915.0
65	14284.5
66	11654.0
67	10059.0
68	8464.0
69	7499.5
70	6535.0
71	5645.0
72	4755.0
73	3687.5
74	2620.0
75	2033.5
76	1447.0
77	1363.5
78	1280.0
79	880.0
80	480.0
81	326.0
82	172.0
83	159.0
84	146.0
85	109.0
86	72.0
87	48.0
88	24.0
89	18.5
90	13.0
91	11.5
92	10.0
93	7.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1263920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.007473336993343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45429908530198	19.869512658851836
2	8.40976502792877	4.206139494126155
3	3.132058601103876	2.349746158710175
4	1.6695681904846351	1.670067280313469
5	0.9952556130469341	1.2444414103382084
6	0.7267617285162451	1.0904684728930314
7	0.514736543451422	0.9010582270156284
8	0.4045995526082355	0.8094410019207906
9	0.3121420762021735	0.7025296183181637
>10	3.2129152450107967	18.41123510737187
>50	0.6832364888215079	11.699584912647214
>100	0.41146931638071527	20.920381250697336
>500	0.06078162368809754	10.513191492326431
>1k	0.012092679058364954	3.8295911722598532
>5k	0.0	0.0
>10k+	3.1822839627276194E-4	1.782611742209815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22400	1.7722640673460344	No Hit
TTCAGAGGGAACGGCATTAGGAGAATTGTTTTAGATATATTGAAGCCGCA	2558	0.20238622697639091	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC	2142	0.16947275143996454	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG	1727	0.13663839483511614	No Hit
TTAGAAGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT	1626	0.1286473827457434	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1610	0.12738147984049625	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1514	0.11978606240901322	No Hit
AGTTAAGGGCAGCCTAACACTTCTATGACAAACCGACGAAAATTATAAAA	1499	0.11859927843534401	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	1469	0.11622571048800556	No Hit
ACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTTTAGCAA	1385	0.10957972023545794	No Hit
AGCTAAGGGGAGGCCAGGAGGATAATTAACTTGAGGAATAGACAACTTAC	1380	0.1091841255775682	No Hit
TTATGAGGGAGGATTATGGGTTTTTGTTTTGTTTTCTCTTAATTTTGTTA	1314	0.10396227609342365	No Hit
TCCTGTGGGTTTTTTTTTTCATTTCATCTGTACATTAATTCTGTTGGGTA	1297	0.10261725425659851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09755364263560985	0.0
2	0.0	0.0	0.0	0.32976770681688716	0.0
3	0.0	0.0	0.0	0.43942654598392306	0.0
4	0.0	0.0	0.0	0.6336635230077853	0.0
5	0.0	0.0	0.0	1.1318754351541236	0.0
6	0.0	0.0	0.0	1.5032596999810115	0.0
7	0.0	0.0	0.0	1.7970282929299324	0.0
8	0.0	0.0	0.0	2.5144787644787643	0.0
9	0.0	0.0	0.0	2.775413000822837	0.0
10	0.0	0.0	0.0	3.400610798151782	0.0
11	0.0	0.0	0.0	3.9648870181657068	0.0
12	0.0	0.0	0.0	4.568406228242294	0.0
13	0.0	0.0	0.0	4.754335717450472	0.0
14	0.0	0.0	0.0	4.827758085954807	0.0
15	0.0	0.0	0.0	4.962497626432053	0.0
16	0.0	0.0	0.0	5.2220868409392995	0.0
17	0.0	0.0	0.0	5.5065193999620226	0.0
18	0.0	0.0	0.0	5.9339989872776755	0.0
19	0.0	0.0	0.0	6.152604595227546	0.0
20	0.0	0.0	0.0	6.3682036837774545	0.0
21	0.0	0.0	0.0	6.648047344768656	0.0
22	0.0	0.0	0.0	6.934220520286094	0.0
23	0.0	0.0	0.0	7.259082853345149	0.0
24	0.0	0.0	0.0	7.504509779099943	0.0
25	0.0	0.0	0.0	7.69969618330274	0.0
26	0.0	0.0	0.0	7.917035888347364	0.0
27	0.0	0.0	0.0	8.18588201784923	0.0
28	0.0	0.0	0.0	8.415959870877904	0.0
29	0.0	0.0	0.0	8.69485410469017	0.0
30	0.0	0.0	0.0	9.016630799417685	0.0
31	0.0	0.0	0.0	9.309212608392937	0.0
32	0.0	0.0	0.0	9.55234508513197	0.0
33	0.0	0.0	0.0	9.807028925881385	0.0
34	0.0	0.0	0.0	10.09715804797772	0.0
35	0.0	0.0	0.0	10.447021963415406	0.0
36	0.0	0.0	0.0	10.700598139122729	0.0
37	0.0	0.0	0.0	11.000854484461042	0.0
38	0.0	0.0	0.0	11.312029242357111	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACC	20	7.856779E-4	44.000004	25
CGTGGAC	20	7.856779E-4	44.000004	25
ATCTCGA	20	7.856779E-4	44.000004	2
ATCTCCG	20	7.856779E-4	44.000004	1
GTCGATC	40	8.312782E-9	44.000004	29
CGACGGC	30	2.5279169E-6	44.000004	19
CGACCGA	20	7.856779E-4	44.000004	25
CGTTATC	30	2.5279169E-6	44.000004	17
CGTTACC	20	7.856779E-4	44.000004	44
CGGATGA	20	7.856779E-4	44.000004	11
CATACCG	20	7.856779E-4	44.000004	1
CAGTACG	30	2.5279169E-6	44.000004	1
ATCGATT	95	0.0	44.000004	14
AACCGAA	70	0.0	44.0	10
TCGTTAG	55	1.8189894E-12	44.0	1
CGAACTG	75	0.0	44.0	36
ATAGACC	25	4.4430926E-5	44.0	38
AAGCGGT	25	4.4430926E-5	44.0	16
TACGGGT	75	0.0	44.0	4
TCGATAG	75	0.0	44.0	1
>>END_MODULE