FastQCFastQC Report
Thu 26 May 2016
SRR1546219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546219_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2078453
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361761.7405252849114219No Hit
GAGAAGGGGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAG35730.1719067017632826No Hit
GGGGAAGGTATCCATGTATCTGCTTACTGCTTTATGAGTTCATTGTTACA29360.1412589074662742No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC26320.1266326445678589No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.12518926336077843No Hit
GTTGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA24590.1183091462736949No Hit
ATAGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG23080.11104412753138992No Hit
ATGTATGGTGGCATGACACAGAAATATATTTTGTGTTTGTATGACTTTTA22850.10993753527262826No Hit
TGTAATGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT22370.10762812534129951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21870.1052224899961654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT750.044.0000049
TCACGAA254.4440865E-544.034
GGTACGA254.4440865E-544.08
CAAGTCG408.3182385E-944.015
GATCGAT254.4440865E-544.016
CGACGGT1150.044.028
ATTACGA207.857952E-444.010
CCGGATT302.52871E-644.05
TAACGTG302.52871E-644.015
CGTTACA207.857952E-444.033
CGGATGT207.857952E-444.031
TACGAAC254.4440865E-544.010
GTCACGA302.52871E-644.014
CTCGCTA207.857952E-444.041
ACGTCGA207.857952E-444.027
ACGTACA302.52871E-644.021
CCGAACT1000.044.012
TCGACAG207.857952E-444.01
GTGCGTA254.4440865E-544.01
TATCGGC254.4440865E-544.024
Produced by FastQC (version 0.11.5)