##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546219_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2078453
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865768915631	31.0	31.0	34.0	30.0	34.0
2	32.215887008270094	33.0	31.0	34.0	30.0	34.0
3	32.094569374433775	33.0	31.0	34.0	30.0	34.0
4	35.88110676546451	37.0	35.0	37.0	35.0	37.0
5	35.876710226307736	37.0	35.0	37.0	35.0	37.0
6	35.934332409729734	37.0	35.0	37.0	35.0	37.0
7	36.17412084853495	37.0	35.0	37.0	35.0	37.0
8	36.07355326293161	37.0	35.0	37.0	35.0	37.0
9	37.82482885107337	39.0	38.0	39.0	35.0	39.0
10	37.455815936179455	39.0	37.0	39.0	35.0	39.0
11	37.29666487527021	39.0	37.0	39.0	34.0	39.0
12	36.71073870806797	39.0	35.0	39.0	33.0	39.0
13	36.46509302832443	39.0	35.0	39.0	33.0	39.0
14	37.518483218047265	40.0	35.0	41.0	33.0	41.0
15	37.74097946886458	40.0	35.0	41.0	33.0	41.0
16	37.855429494917615	40.0	35.0	41.0	33.0	41.0
17	37.78324407624324	40.0	35.0	41.0	33.0	41.0
18	37.56514965698046	39.0	36.0	41.0	33.0	41.0
19	37.29783690081036	38.0	35.0	41.0	33.0	41.0
20	36.99454594354551	38.0	35.0	41.0	33.0	41.0
21	36.884378910660956	38.0	35.0	41.0	33.0	41.0
22	36.81820036344339	38.0	35.0	41.0	33.0	41.0
23	36.80117327647053	38.0	35.0	41.0	33.0	41.0
24	36.73408058782181	38.0	35.0	41.0	33.0	41.0
25	36.721370654039326	38.0	35.0	41.0	33.0	41.0
26	36.64318173179764	38.0	35.0	41.0	33.0	41.0
27	36.57318977143096	38.0	35.0	41.0	32.0	41.0
28	36.479142900994155	38.0	35.0	41.0	32.0	41.0
29	36.37122994842799	38.0	35.0	41.0	32.0	41.0
30	36.13959035879089	37.0	35.0	41.0	31.0	41.0
31	35.79506296269389	37.0	35.0	41.0	30.0	41.0
32	35.393963683566575	37.0	35.0	41.0	27.0	41.0
33	34.93597449641633	37.0	35.0	41.0	23.0	41.0
34	34.414523686607296	37.0	34.0	40.0	21.0	41.0
35	34.15305085080105	37.0	34.0	40.0	18.0	41.0
36	33.986813269292114	37.0	34.0	40.0	18.0	41.0
37	33.93357511572309	37.0	34.0	40.0	18.0	41.0
38	33.870714420773524	37.0	33.0	40.0	18.0	41.0
39	33.81822826881339	37.0	34.0	40.0	17.0	41.0
40	33.659129169627604	37.0	33.0	40.0	16.0	41.0
41	33.55125422609989	37.0	33.0	40.0	15.0	41.0
42	33.48414999040151	36.0	33.0	40.0	15.0	41.0
43	33.37261559438679	36.0	33.0	40.0	15.0	41.0
44	33.24684176163714	36.0	33.0	40.0	14.0	41.0
45	33.20607394057022	36.0	33.0	40.0	15.0	41.0
46	33.15423827240741	36.0	33.0	40.0	13.0	41.0
47	33.1100125910954	35.0	33.0	40.0	13.0	41.0
48	33.03517808677896	35.0	33.0	40.0	12.0	41.0
49	32.9445371148638	36.0	33.0	40.0	11.0	41.0
50	32.78776955745451	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	1.0
12	1.0
13	13.0
14	21.0
15	52.0
16	146.0
17	367.0
18	913.0
19	1805.0
20	3285.0
21	5336.0
22	8441.0
23	12779.0
24	20487.0
25	34146.0
26	53018.0
27	65492.0
28	63260.0
29	55249.0
30	52567.0
31	54367.0
32	62757.0
33	80428.0
34	149671.0
35	282651.0
36	110786.0
37	141032.0
38	238601.0
39	580774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.02437245393569	21.282559672987553	27.13845345552678	20.554614417549978
2	39.343300040943916	21.813194717417232	28.599876927695746	10.24362831394311
3	25.728125678088464	22.867584689189506	40.77648135416101	10.627808278561025
4	23.154528873157105	25.310940396535308	39.796473627260276	11.738057103047314
5	20.176256090467284	27.759780952468017	39.910741306154144	12.153221650910558
6	17.28362392606424	38.65528833223556	36.350208544528066	7.710879197172127
7	76.68491902390865	3.508907827119497	18.054389490645207	1.7517836583266495
8	76.78162556478304	2.772687186094658	18.007383376001286	2.4383038731210185
9	72.68747477089931	4.579126879462754	19.792845929159814	2.9405524204781153
10	40.22073147672812	24.484797106309355	25.853988519345876	9.44048289761664
11	34.008370648746926	21.513308215292817	32.492050578002	11.986270557958251
12	32.626188804846684	18.846180308142642	36.72962535116262	11.798005535848057
13	18.51299981284157	32.457938668808	35.816253723322106	13.212807795028322
14	12.574929526912564	35.028889274859715	38.710569832466746	13.685611365760977
15	12.465809907657281	22.266849430802623	52.69957992795603	12.567760733584066
16	14.793213991367619	19.9576319503015	48.703627168860685	16.545526889470196
17	14.65402393029816	20.226293305646074	37.50212297319208	27.617559790863687
18	15.962930121585622	23.671644246947128	44.634302531738754	15.7311230997285
19	20.065356301056603	24.112500980296403	37.428558644337876	18.393584074309114
20	20.79060724490763	23.224677199821215	39.86498612188969	16.119729433381462
21	17.481896391210196	25.12209802194228	41.42143219019145	15.97457339665607
22	19.202310564636292	22.429951507202713	36.458317796938395	21.909420131222596
23	14.700452692459248	25.34380137534984	36.04955223909321	23.906193693097702
24	14.17130914194355	22.901119245900677	48.60037729984753	14.327194312308242
25	15.316776467882603	25.21827532304074	42.530430084298274	16.934518124778382
26	13.84087107093593	27.551164255337984	37.89741697310451	20.710547700621568
27	14.232845294072083	30.691528747582936	37.39194487438494	17.683681083960042
28	12.609907464830814	27.60947685610404	41.23682373380586	18.543791945259287
29	13.624941242356694	23.69762510867458	41.11639762842845	21.56103602054028
30	15.600497100487717	28.651213185960906	37.804463223368536	17.943826490182843
31	19.24224411136552	30.110615924439955	34.30902695418179	16.338113010012737
32	21.288814324884903	24.7313266164787	36.25619631524023	17.723662743396172
33	20.866769659934576	29.387145150744328	32.36137646605432	17.384708723266776
34	15.91751172626949	27.691845810321425	34.44533025283709	21.945312210572
35	17.183116481344538	27.853023378445414	34.60193711380531	20.361923026404735
36	23.708258016900068	28.346804089387632	31.658016803844014	16.28692108986828
37	16.880680005754282	31.619430412908063	36.16795761078071	15.331931970556948
38	16.989703399595758	29.60779002460003	34.122253425985576	19.280253149818638
39	16.703913920593827	29.458881196736225	35.24953414871542	18.587670733954536
40	19.452929654892365	25.799861724080365	34.22025900994634	20.526949611080934
41	14.560877729734568	25.93760840394274	36.055903116404366	23.445610749918327
42	16.730616472924815	26.50966848901563	33.75808834743918	23.00162669062038
43	16.863311318562413	25.600049652313523	35.19882335563999	22.337815673484076
44	15.424789494879123	28.18158505388383	33.53672178297994	22.85690366825711
45	15.946427463118	34.41588527621264	29.625206824498797	20.012480436170556
46	19.723082504150923	29.474998953548624	33.05973240674675	17.742186135553702
47	16.789265862639184	29.535957753194324	33.754191218180054	19.92058516598643
48	17.668958595647823	27.79918525942131	34.1154695343123	20.41638661061857
49	19.582160385632967	26.179182305301108	35.24770586585311	18.99095144321281
50	18.59941023443879	28.615321106611503	32.12990623314552	20.655362425804192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4686.0
1	9226.5
2	13767.0
3	39801.5
4	65836.0
5	46963.0
6	28090.0
7	28442.5
8	28795.0
9	30523.0
10	32251.0
11	31927.5
12	31604.0
13	29498.0
14	27392.0
15	25601.5
16	23811.0
17	21039.0
18	18267.0
19	17752.5
20	17238.0
21	17720.5
22	18203.0
23	17667.0
24	17131.0
25	21780.5
26	26430.0
27	36133.0
28	45836.0
29	49494.5
30	53153.0
31	61129.0
32	69105.0
33	89032.0
34	108959.0
35	103510.5
36	98062.0
37	105703.0
38	113344.0
39	119664.5
40	125985.0
41	148219.5
42	170454.0
43	165704.5
44	160955.0
45	152431.0
46	143907.0
47	139994.5
48	136082.0
49	125748.0
50	115414.0
51	99878.5
52	84343.0
53	72271.5
54	60200.0
55	54234.5
56	48269.0
57	46610.5
58	44952.0
59	41959.5
60	38967.0
61	35147.0
62	31327.0
63	28586.0
64	25845.0
65	21729.5
66	17614.0
67	14479.5
68	11345.0
69	10069.5
70	8794.0
71	6956.5
72	5119.0
73	4211.5
74	3304.0
75	2480.5
76	1657.0
77	1265.5
78	874.0
79	744.0
80	614.0
81	370.5
82	127.0
83	167.5
84	208.0
85	138.5
86	69.0
87	45.5
88	22.0
89	15.0
90	8.0
91	13.5
92	19.0
93	17.0
94	15.0
95	8.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2078453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.7266322411094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76439439080893	17.900494250136145
2	8.662231874674033	3.937267164058647
3	3.321513390176298	2.2646043990737166
4	1.8182217103460625	1.652882245753436
5	1.0922087313667121	1.2411113084149958
6	0.7579751642383913	1.0335733683324249
7	0.577423717091482	0.9186027525942754
8	0.435668051841569	0.7921014074723136
9	0.3443966226365269	0.704427784896643
>10	2.9028273150135266	14.334665589410791
>50	0.7393919784990557	11.98692936818123
>100	0.49146429699797956	21.840492793067305
>500	0.061379754773933246	9.687285162137483
>1k	0.030689877386966623	9.953343788529326
>5k	0.0	0.0
>10k+	2.1312414852060155E-4	1.7522186179412487
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36176	1.7405252849114219	No Hit
GAGAAGGGGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAG	3573	0.1719067017632826	No Hit
GGGGAAGGTATCCATGTATCTGCTTACTGCTTTATGAGTTCATTGTTACA	2936	0.1412589074662742	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2632	0.1266326445678589	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2602	0.12518926336077843	No Hit
GTTGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	2459	0.1183091462736949	No Hit
ATAGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2308	0.11104412753138992	No Hit
ATGTATGGTGGCATGACACAGAAATATATTTTGTGTTTGTATGACTTTTA	2285	0.10993753527262826	No Hit
TGTAATGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT	2237	0.10762812534129951	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2187	0.1052224899961654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07149548245738538	0.0
2	0.0	0.0	0.0	0.3094128180911476	0.0
3	0.0	0.0	0.0	0.4672706094388471	0.0
4	0.0	0.0	0.0	0.7488261702333419	0.0
5	0.0	0.0	0.0	1.3259380895310118	0.0
6	0.0	0.0	0.0	1.8672541548930863	0.0
7	0.0	0.0	0.0	2.2104420932299167	0.0
8	0.0	0.0	0.0	2.9922254676915956	0.0
9	0.0	0.0	0.0	3.2861941068669824	0.0
10	0.0	0.0	0.0	3.7729984753083183	0.0
11	0.0	0.0	0.0	4.362956487349004	0.0
12	0.0	0.0	0.0	4.8393685110993605	0.0
13	0.0	0.0	0.0	5.010986536621227	0.0
14	0.0	0.0	0.0	5.076034916353653	0.0
15	0.0	0.0	0.0	5.178081967694242	0.0
16	0.0	0.0	0.0	5.413112540913843	0.0
17	0.0	0.0	0.0	5.686825730483201	0.0
18	0.0	0.0	0.0	6.119743867193533	0.0
19	0.0	0.0	0.0	6.304496661699831	0.0
20	0.0	0.0	0.0	6.5277877344351785	0.0
21	0.0	0.0	0.0	6.818099807885961	0.0
22	0.0	0.0	0.0	7.10672793659515	0.0
23	0.0	0.0	0.0	7.405892748116027	0.0
24	0.0	0.0	0.0	7.656656176492805	0.0
25	0.0	0.0	0.0	7.859451236087609	0.0
26	0.0	0.0	0.0	8.095492176152167	0.0
27	0.0	0.0	0.0	8.344427321666643	0.0
28	0.0	0.0	0.0	8.580708825265715	0.0
29	0.0	0.0	0.0	8.90498846978979	0.0
30	0.0	0.0	0.0	9.261696078766274	0.0
31	0.0	0.0	0.0	9.605557594999743	0.0
32	0.0	0.0	0.0	9.889278227604859	0.0
33	0.0	0.0	0.0	10.155678285725008	0.0
34	0.0	0.0	0.0	10.447722416624288	0.0
35	0.0	0.0	0.0	10.802938531686788	0.0
36	0.0	0.0	0.0	11.082569584205176	0.0
37	0.0	0.0	0.0	11.373410897431889	0.0
38	0.0	0.0	0.0	11.694418877886582	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	75	0.0	44.000004	9
TCACGAA	25	4.4440865E-5	44.0	34
GGTACGA	25	4.4440865E-5	44.0	8
CAAGTCG	40	8.3182385E-9	44.0	15
GATCGAT	25	4.4440865E-5	44.0	16
CGACGGT	115	0.0	44.0	28
ATTACGA	20	7.857952E-4	44.0	10
CCGGATT	30	2.52871E-6	44.0	5
TAACGTG	30	2.52871E-6	44.0	15
CGTTACA	20	7.857952E-4	44.0	33
CGGATGT	20	7.857952E-4	44.0	31
TACGAAC	25	4.4440865E-5	44.0	10
GTCACGA	30	2.52871E-6	44.0	14
CTCGCTA	20	7.857952E-4	44.0	41
ACGTCGA	20	7.857952E-4	44.0	27
ACGTACA	30	2.52871E-6	44.0	21
CCGAACT	100	0.0	44.0	12
TCGACAG	20	7.857952E-4	44.0	1
GTGCGTA	25	4.4440865E-5	44.0	1
TATCGGC	25	4.4440865E-5	44.0	24
>>END_MODULE