FastQCFastQC Report
Thu 26 May 2016
SRR1546218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546218_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2134150
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368251.7255113276948668No Hit
GAGAAGGGGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAG37220.17440198673945131No Hit
GGGGAAGGTATCCATGTATCTGCTTACTGCTTTATGAGTTCATTGTTACA30890.1447414661574866No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.12440550101914111No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC26060.12210950495513437No Hit
GTTGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA25880.12126607782958086No Hit
ATAGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG23880.11189466532343087No Hit
TGTAATGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT22860.10711524494529437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22840.10702153082023289No Hit
ATGTATGGTGGCATGACACAGAAATATATTTTGTGTTTGTATGACTTTTA21970.10294496638005764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC207.8580005E-444.033
TACCCGC207.8580005E-444.024
CGAGTAA408.3182385E-944.036
GGCGTAA800.044.09
ATTACGC207.8580005E-444.038
GCTATCG408.3182385E-944.022
CCGTGCC254.4441273E-544.022
CCTACCG302.5287409E-644.018
CCGCTAT254.4441273E-544.030
CCCGTAT254.4441273E-544.034
TCGGACG254.4441273E-544.01
ACCTACG254.4441273E-544.025
TACGATT254.4441273E-544.027
CATACCC207.8580005E-444.036
GTATCGA454.802132E-1044.044
ACTCGAC254.4441273E-544.011
CAAGCCG207.8580005E-444.042
CGTGTCA800.044.018
AGTTACG1550.044.01
ACGTATG454.802132E-1044.01
Produced by FastQC (version 0.11.5)