##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546218_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2134150
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80303961764637	31.0	31.0	34.0	30.0	34.0
2	32.18508820842021	33.0	31.0	34.0	30.0	34.0
3	32.189079024435955	33.0	31.0	34.0	30.0	34.0
4	35.91689337675421	37.0	35.0	37.0	35.0	37.0
5	35.83400745027294	37.0	35.0	37.0	35.0	37.0
6	35.91498816859171	37.0	35.0	37.0	35.0	37.0
7	36.14907949300658	37.0	35.0	37.0	35.0	37.0
8	36.05269779537521	37.0	35.0	37.0	35.0	37.0
9	37.793538879647635	39.0	38.0	39.0	35.0	39.0
10	37.450590164702575	39.0	37.0	39.0	35.0	39.0
11	37.28799803200337	39.0	37.0	39.0	34.0	39.0
12	36.68810392896469	39.0	35.0	39.0	33.0	39.0
13	36.40115127802638	39.0	35.0	39.0	33.0	39.0
14	37.43632968629197	40.0	35.0	41.0	33.0	41.0
15	37.693976056041045	40.0	35.0	41.0	33.0	41.0
16	37.799732446172946	40.0	35.0	41.0	33.0	41.0
17	37.73857226530469	40.0	35.0	41.0	33.0	41.0
18	37.5374875243071	39.0	36.0	41.0	33.0	41.0
19	37.27581051003913	38.0	35.0	41.0	33.0	41.0
20	36.972360424524986	38.0	35.0	41.0	33.0	41.0
21	36.86162500292857	38.0	35.0	41.0	33.0	41.0
22	36.80634491483729	38.0	35.0	41.0	33.0	41.0
23	36.776520863107095	38.0	35.0	41.0	33.0	41.0
24	36.71336082281002	38.0	35.0	41.0	33.0	41.0
25	36.69409601012113	38.0	35.0	41.0	33.0	41.0
26	36.629827800295196	38.0	35.0	41.0	33.0	41.0
27	36.54694749666143	38.0	35.0	41.0	32.0	41.0
28	36.44832275144671	38.0	35.0	41.0	32.0	41.0
29	36.34615233231029	37.0	35.0	41.0	32.0	41.0
30	36.11756436988965	37.0	35.0	41.0	31.0	41.0
31	35.785587236136166	37.0	35.0	41.0	30.0	41.0
32	35.39855164819718	37.0	35.0	41.0	28.0	41.0
33	34.92303212051637	37.0	35.0	41.0	23.0	41.0
34	34.48473537473936	37.0	34.0	41.0	21.0	41.0
35	34.18789307218331	37.0	34.0	41.0	18.0	41.0
36	34.009285664081716	37.0	34.0	40.0	18.0	41.0
37	33.888379917063	37.0	34.0	40.0	18.0	41.0
38	33.83480730033034	37.0	33.0	40.0	17.0	41.0
39	33.786465337487996	37.0	34.0	40.0	17.0	41.0
40	33.64851205397934	37.0	33.0	40.0	15.0	41.0
41	33.56950636084624	37.0	33.0	40.0	15.0	41.0
42	33.483634233769884	36.0	33.0	40.0	15.0	41.0
43	33.400089965560056	36.0	33.0	40.0	15.0	41.0
44	33.264439706674786	36.0	33.0	40.0	14.0	41.0
45	33.21323571445306	36.0	33.0	40.0	15.0	41.0
46	33.171904505306564	36.0	33.0	40.0	14.0	41.0
47	33.10830447719233	35.0	33.0	40.0	13.0	41.0
48	33.05535974509758	35.0	33.0	40.0	12.0	41.0
49	32.96247967574913	36.0	33.0	40.0	12.0	41.0
50	32.78101445540379	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	7.0
13	8.0
14	22.0
15	69.0
16	168.0
17	395.0
18	974.0
19	1948.0
20	3373.0
21	5601.0
22	8637.0
23	13443.0
24	20957.0
25	34643.0
26	53897.0
27	66318.0
28	65182.0
29	57094.0
30	54646.0
31	56690.0
32	65477.0
33	84184.0
34	156411.0
35	285670.0
36	115240.0
37	146857.0
38	246603.0
39	589631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.085443853524826	21.376285640653187	27.216924770986108	20.321345734835884
2	39.41826956868074	21.791064358175387	28.567064170747138	10.223601902396737
3	25.712859920811564	22.88480191176815	40.730595318979454	10.67174284844083
4	23.31232575029871	25.275355527961953	39.640184616826375	11.772134104912963
5	20.226413326148585	27.768807253473284	39.824942014385115	12.179837405993018
6	17.3258674413701	38.66152800880913	36.2370967364056	7.775507813415176
7	76.76451983225172	3.520839678560551	17.9655600590399	1.749080430147834
8	76.83133800342057	2.7736569594452125	17.918234425883842	2.4767706112503807
9	72.70407422158705	4.597614975517185	19.69449195229951	3.003818850596256
10	40.27430124405501	24.451561511608837	25.755124991214302	9.519012253121852
11	34.09572897875032	21.50701684511398	32.365766230115035	12.031487946020663
12	32.71311763465548	18.828714007918844	36.64850174542558	11.809666612000095
13	18.650563456176933	32.43328725722184	35.71220392193614	13.20394536466509
14	12.638380619918937	35.051613054377626	38.606611531523086	13.703394794180355
15	12.482346601691539	22.23704987934306	52.682894829323146	12.597708689642245
16	14.917226999039428	19.971792048356487	48.555162476864325	16.555818475739756
17	14.741044443923808	20.1686854251107	37.382517629969776	27.707752500995714
18	16.05252676709697	23.603542393927324	44.58149614600661	15.7624346929691
19	20.152191739099877	24.083265000117144	37.339737131879204	18.42480612890378
20	20.83864770517536	23.251364711946206	39.77246210435068	16.13752547852775
21	17.48822716303915	25.13590890987044	41.411569008738844	15.964294918351568
22	19.198228803036336	22.45109294098353	36.42246327577724	21.92821498020289
23	14.69175081414146	25.417379284492654	35.9692149099173	23.921654991448584
24	14.23494131152918	22.924536700794228	48.41402900452171	14.426492983154887
25	15.420565564744745	25.13084834711712	42.39776960382354	17.0508164843146
26	13.854368249654428	27.5253379565635	37.781036946793805	20.839256846988263
27	14.208139071761591	30.675538270505818	37.359276526954524	17.75704613077806
28	12.644331466860342	27.646229177892838	41.09125412927864	18.618185225968183
29	13.698146803176908	23.73188388819905	40.95087036993651	21.619098938687532
30	15.65274230958461	28.650141742614153	37.6813719747909	18.01574397301033
31	19.251973853759107	30.11728322751447	34.26942810955181	16.361314809174612
32	21.381721059906756	24.673242274441815	36.18185225968184	17.76318440596959
33	20.894595037837078	29.365695944521235	32.28671836562566	17.452990652016027
34	15.95750064428461	27.6735937024108	34.30175948269803	22.067146170606563
35	17.181641402900453	27.821099735257597	34.444720380479346	20.552538481362603
36	23.799358058243328	28.315722887332196	31.524119672937704	16.360799381486775
37	16.879975634327486	31.666143429468406	36.02994166295715	15.423939273246958
38	17.044865637373192	29.570461307780615	33.96743434154113	19.417238713305064
39	16.724363329662864	29.42000327999438	35.1651008598271	18.690532530515664
40	19.499426000984	25.729728463322633	34.15251973853759	20.618325797155777
41	14.56369046224492	25.83890541901928	36.02600566970457	23.571398449031232
42	16.691891385329054	26.44664152004311	33.821615162945434	23.039851931682403
43	16.872947074947874	25.51142140899187	35.135065482744885	22.480566033315373
44	15.44830494576295	28.009090270130965	33.56085560996181	22.981749174144273
45	15.924888128763207	34.39167818569454	29.53831736288452	20.14511632265773
46	19.809713469062622	29.42632898343603	32.95508750556428	17.808870041937073
47	16.78968207483073	29.53822364875946	33.61895836749994	20.05313590890987
48	17.583534428226695	27.895930464119207	34.061804465478055	20.458730642176043
49	19.64065318745168	26.095166693999953	35.16950542370499	19.09467469484338
50	18.611531523088818	28.570531593374408	32.00974626900639	20.808190614530375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4868.0
1	9503.5
2	14139.0
3	40868.5
4	67598.0
5	47812.0
6	28026.0
7	28861.0
8	29696.0
9	31106.5
10	32517.0
11	32128.0
12	31739.0
13	29709.5
14	27680.0
15	25832.5
16	23985.0
17	21182.5
18	18380.0
19	17986.0
20	17592.0
21	17978.0
22	18364.0
23	18067.5
24	17771.0
25	22358.0
26	26945.0
27	36698.5
28	46452.0
29	50164.5
30	53877.0
31	61832.5
32	69788.0
33	90486.5
34	111185.0
35	105957.0
36	100729.0
37	108059.0
38	115389.0
39	122404.0
40	129419.0
41	152115.0
42	174811.0
43	169829.0
44	164847.0
45	156181.0
46	147515.0
47	144529.0
48	141543.0
49	131337.0
50	121131.0
51	104915.5
52	88700.0
53	75859.0
54	63018.0
55	56574.5
56	50131.0
57	48454.5
58	46778.0
59	43446.0
60	40114.0
61	36041.0
62	31968.0
63	29216.0
64	26464.0
65	22202.5
66	17941.0
67	14792.5
68	11644.0
69	10242.5
70	8841.0
71	7163.5
72	5486.0
73	4426.0
74	3366.0
75	2513.5
76	1661.0
77	1295.0
78	929.0
79	773.0
80	617.0
81	385.5
82	154.0
83	185.0
84	216.0
85	149.0
86	82.0
87	48.5
88	15.0
89	12.5
90	10.0
91	10.5
92	11.0
93	13.0
94	15.0
95	8.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2134150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.69001741857496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.750695088838	17.8685464329062
2	8.583263562610469	3.895087994877026
3	3.3890785767254155	2.3069475581645675
4	1.7961725892029368	1.630207493431261
5	1.1093529431752938	1.2585618801997405
6	0.7795166338739421	1.06123476004012
7	0.576914575361821	0.9163141234792647
8	0.4476284285302498	0.8125357472320565
9	0.3510047962417764	0.7167874446656336
>10	2.8949886113802337	14.199349201051135
>50	0.7399046961588408	11.951568498122386
>100	0.4883511108706714	21.77720574408591
>500	0.06125222771122834	9.560124743838477
>1k	0.03166781840852621	10.304715291620749
>5k	0.0	0.0
>10k+	2.0834091058240933E-4	1.7408130862854512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36825	1.7255113276948668	No Hit
GAGAAGGGGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAAGAG	3722	0.17440198673945131	No Hit
GGGGAAGGTATCCATGTATCTGCTTACTGCTTTATGAGTTCATTGTTACA	3089	0.1447414661574866	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2655	0.12440550101914111	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2606	0.12210950495513437	No Hit
GTTGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	2588	0.12126607782958086	No Hit
ATAGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2388	0.11189466532343087	No Hit
TGTAATGGGAAGTTTAGTATAGAAACAATTATCATGGAAATAACTACTGT	2286	0.10711524494529437	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2284	0.10702153082023289	No Hit
ATGTATGGTGGCATGACACAGAAATATATTTTGTGTTTGTATGACTTTTA	2197	0.10294496638005764	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08017243399011316	0.0
2	0.0	0.0	0.0	0.33024857671672564	0.0
3	0.0	0.0	0.0	0.49518543682496546	0.0
4	0.0	0.0	0.0	0.7859335098282688	0.0
5	0.0	0.0	0.0	1.3871096220977908	0.0
6	0.0	0.0	0.0	1.9418972424618701	0.0
7	0.0	0.0	0.0	2.2914509289412646	0.0
8	0.0	0.0	0.0	3.0779935805824334	0.0
9	0.0	0.0	0.0	3.378862779092379	0.0
10	0.0	0.0	0.0	3.8896047606775532	0.0
11	0.0	0.0	0.0	4.48923458988356	0.0
12	0.0	0.0	0.0	4.98409202727081	0.0
13	0.0	0.0	0.0	5.165100859827097	0.0
14	0.0	0.0	0.0	5.234215027059953	0.0
15	0.0	0.0	0.0	5.341705128505494	0.0
16	0.0	0.0	0.0	5.587048707916501	0.0
17	0.0	0.0	0.0	5.874095072979875	0.0
18	0.0	0.0	0.0	6.329498863716234	0.0
19	0.0	0.0	0.0	6.524658529156807	0.0
20	0.0	0.0	0.0	6.765597544689923	0.0
21	0.0	0.0	0.0	7.069934165827144	0.0
22	0.0	0.0	0.0	7.378628493779725	0.0
23	0.0	0.0	0.0	7.6992713726776465	0.0
24	0.0	0.0	0.0	7.9675280556661905	0.0
25	0.0	0.0	0.0	8.187568821310592	0.0
26	0.0	0.0	0.0	8.44045638778905	0.0
27	0.0	0.0	0.0	8.715085631281775	0.0
28	0.0	0.0	0.0	8.966801771196964	0.0
29	0.0	0.0	0.0	9.300752055853618	0.0
30	0.0	0.0	0.0	9.67762340978844	0.0
31	0.0	0.0	0.0	10.027411381580489	0.0
32	0.0	0.0	0.0	10.317456598645832	0.0
33	0.0	0.0	0.0	10.597614975517185	0.0
34	0.0	0.0	0.0	10.90790244359581	0.0
35	0.0	0.0	0.0	11.280556661902866	0.0
36	0.0	0.0	0.0	11.563526462526065	0.0
37	0.0	0.0	0.0	11.861537380221634	0.0
38	0.0	0.0	0.0	12.198533373942787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	20	7.8580005E-4	44.0	33
TACCCGC	20	7.8580005E-4	44.0	24
CGAGTAA	40	8.3182385E-9	44.0	36
GGCGTAA	80	0.0	44.0	9
ATTACGC	20	7.8580005E-4	44.0	38
GCTATCG	40	8.3182385E-9	44.0	22
CCGTGCC	25	4.4441273E-5	44.0	22
CCTACCG	30	2.5287409E-6	44.0	18
CCGCTAT	25	4.4441273E-5	44.0	30
CCCGTAT	25	4.4441273E-5	44.0	34
TCGGACG	25	4.4441273E-5	44.0	1
ACCTACG	25	4.4441273E-5	44.0	25
TACGATT	25	4.4441273E-5	44.0	27
CATACCC	20	7.8580005E-4	44.0	36
GTATCGA	45	4.802132E-10	44.0	44
ACTCGAC	25	4.4441273E-5	44.0	11
CAAGCCG	20	7.8580005E-4	44.0	42
CGTGTCA	80	0.0	44.0	18
AGTTACG	155	0.0	44.0	1
ACGTATG	45	4.802132E-10	44.0	1
>>END_MODULE