SRR1546217_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546217_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1509055
Sequences flagged as poor quality	0
Sequence length	50
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18677	1.237661980510982	No Hit
TTTGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2573	0.17050405717485448	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2329	0.15433499773036768	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2259	0.14969633313563785	No Hit
ATGTCTGGGAATGAAAGAGAGAGAGAGAGAAAGAAAAAAGAGAAAAAAGA	2136	0.1415455367763269	No Hit
ACAATTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	2106	0.13955753766429985	No Hit
TATGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1995	0.13220194094979973	No Hit
TTCGTTGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT	1935	0.12822594272574558	No Hit
AGGGGAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCG	1934	0.128159676088678	No Hit
GATTAAGGGGAGTTTGTGAAATCTGGATTAAAAAATTACCTCTTCAGTGT	1887	0.12504514414650228	No Hit
GGACTGGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT	1860	0.12325594494567793	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG	1857	0.12305714503447521	TruSeq Adapter, Index 22 (95% over 21bp)
TAAGTAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA	1740	0.11530394849756968	No Hit
TTTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1687	0.11179181673298853	No Hit
ATTAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1602	0.10615915258224518	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1600	0.10602661930811004	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	1596	0.10576155275983977	TruSeq Adapter, Index 20 (95% over 21bp)
TATACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1553	0.10291208736593431	No Hit
GTTTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1549	0.10264702081766404	No Hit
AATACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1537	0.1018518211728532	No Hit
GGAAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1528	0.1012554214392451	No Hit
TTTGCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	1520	0.10072528834270454	No Hit
TTAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1518	0.1005927550685694	No Hit
TTTTTAGGGAATCAATCTAATAGGAGAATTATTCATTACCATATCATTAT	1515	0.10039395515736668	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	40	8.314601E-9	44.000004	27
GCCCCTA	145	0.0	44.000004	13
AGCGTTG	20	7.8572653E-4	44.000004	1
AACGTAC	65	0.0	44.000004	16
CGGGTAC	40	8.314601E-9	44.000004	6
CTGTCGA	20	7.8572653E-4	44.000004	12
ATCTCGC	40	8.314601E-9	44.000004	13
GGTACGA	40	8.314601E-9	44.000004	8
CGAACTC	40	8.314601E-9	44.000004	13
TACCCGC	130	0.0	44.000004	40
TACCCGA	20	7.8572653E-4	44.000004	33
CAAACGT	65	0.0	44.000004	13
CGAACAT	80	0.0	44.000004	11
ATTAGTC	30	2.5282443E-6	44.000004	44
CTCCGTC	20	7.8572653E-4	44.000004	11
GTCGACA	30	2.5282443E-6	44.000004	18
GATCGAA	20	7.8572653E-4	44.000004	10
GAATGGA	20	7.8572653E-4	44.000004	3
CACGGCA	110	0.0	44.000004	4
CTATCCG	20	7.8572653E-4	44.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content