##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546217_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1509055 Sequences flagged as poor quality 0 Sequence length 50 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948176176481308 33.0 31.0 34.0 30.0 34.0 2 32.32151512038991 33.0 31.0 34.0 31.0 34.0 3 32.25075891866101 34.0 31.0 34.0 30.0 34.0 4 35.98846761715113 37.0 35.0 37.0 35.0 37.0 5 35.990078559098244 37.0 35.0 37.0 35.0 37.0 6 36.04504805987853 37.0 35.0 37.0 35.0 37.0 7 36.21206781727638 37.0 35.0 37.0 35.0 37.0 8 36.12183982691155 37.0 36.0 37.0 35.0 37.0 9 37.9304889483816 39.0 39.0 39.0 35.0 39.0 10 37.594485953129606 39.0 37.0 39.0 35.0 39.0 11 37.46486973635818 39.0 37.0 39.0 35.0 39.0 12 36.889562673328676 39.0 35.0 39.0 33.0 39.0 13 36.65649959743018 39.0 35.0 39.0 33.0 39.0 14 37.86944677298044 40.0 35.0 41.0 33.0 41.0 15 38.03656062900292 40.0 35.0 41.0 33.0 41.0 16 38.14913638005242 40.0 35.0 41.0 34.0 41.0 17 38.05705822518066 40.0 35.0 41.0 34.0 41.0 18 37.88347078138305 39.0 36.0 41.0 34.0 41.0 19 37.63868712538642 38.0 36.0 41.0 34.0 41.0 20 37.2869882144786 38.0 35.0 41.0 34.0 41.0 21 37.21752354950615 38.0 35.0 41.0 33.0 41.0 22 37.15842166123832 38.0 35.0 41.0 33.0 41.0 23 37.1148851433513 38.0 35.0 41.0 33.0 41.0 24 37.024560403696356 38.0 35.0 41.0 33.0 41.0 25 37.02734227712045 38.0 35.0 41.0 33.0 41.0 26 36.940132069407674 38.0 35.0 41.0 33.0 41.0 27 36.90617174324329 38.0 35.0 41.0 33.0 41.0 28 36.84217937715988 38.0 35.0 41.0 33.0 41.0 29 36.74661162118014 38.0 35.0 41.0 33.0 41.0 30 36.55457885895478 38.0 35.0 41.0 32.0 41.0 31 36.21685226847266 38.0 35.0 41.0 31.0 41.0 32 35.85921122821898 38.0 35.0 41.0 30.0 41.0 33 35.427525835705126 38.0 35.0 41.0 27.0 41.0 34 34.93178777446813 38.0 35.0 41.0 23.0 41.0 35 34.66689086878875 38.0 35.0 41.0 21.0 41.0 36 34.39608496708205 38.0 34.0 41.0 18.0 41.0 37 34.37271736285291 38.0 34.0 41.0 18.0 41.0 38 34.38399925781366 38.0 34.0 41.0 18.0 41.0 39 34.317683583434665 38.0 34.0 41.0 18.0 41.0 40 34.151661138924695 38.0 34.0 40.0 18.0 41.0 41 34.02302235505002 38.0 34.0 40.0 16.0 41.0 42 33.960419600345915 38.0 34.0 41.0 15.0 41.0 43 33.831213574057934 37.0 34.0 40.0 15.0 41.0 44 33.62368369608795 37.0 34.0 40.0 14.0 41.0 45 33.539777542899365 37.0 34.0 40.0 14.0 41.0 46 33.4341226794252 37.0 33.0 40.0 12.0 41.0 47 33.46334494103926 37.0 33.0 40.0 12.0 41.0 48 33.44612356739814 37.0 33.0 40.0 12.0 41.0 49 33.29443393381951 36.0 33.0 40.0 10.0 41.0 50 33.16511989291311 36.0 33.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 1.0 10 1.0 11 2.0 12 3.0 13 10.0 14 13.0 15 34.0 16 74.0 17 159.0 18 494.0 19 968.0 20 1881.0 21 3140.0 22 5175.0 23 8471.0 24 14053.0 25 24590.0 26 38128.0 27 44731.0 28 40219.0 29 33661.0 30 31940.0 31 33480.0 32 39840.0 33 51917.0 34 98695.0 35 217074.0 36 75151.0 37 96475.0 38 170424.0 39 478250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.800510915771792 22.5490124614411 29.153741911328613 20.496734711458494 2 33.94243417237941 24.74435988085259 30.95327870753551 10.3599272392325 3 24.500498656443934 23.141634996736368 42.36684547614236 9.991020870677344 4 23.199220704348086 26.249142675382934 38.810447598000074 11.741189022268903 5 19.413805328500285 30.38418082839923 38.90348595644294 11.298527886657544 6 15.32793702018813 40.13180434112739 36.017507645513255 8.522750993171222 7 77.70458995861648 4.575976356063894 16.052496429884926 1.6669372554346924 8 79.04363989384085 3.761426853229339 15.299707432797346 1.8952258201324668 9 76.35573256110612 4.360676052231363 16.584551258900436 2.6990401277620766 10 38.12856390257479 29.899970511346506 23.362501698082575 8.60896388799613 11 33.26737594057208 19.068688682652386 32.988923531614155 14.675011845161375 12 34.74273634824443 18.939535007007695 36.44804198654125 9.869686658206627 13 16.354606028275974 39.71664385989907 29.76187083969769 14.16687927212726 14 9.09927073565907 40.445775667553534 32.89084890875416 17.56410468803324 15 7.96352684295801 26.340060501439645 52.82551000460553 12.870902650996815 16 9.720984324627002 19.890925115386782 46.319584110585765 24.068506449400452 17 9.24021987270179 23.9934263496029 34.39437263718022 32.37198114051509 18 13.075268959713199 27.668574041370263 43.72246207063361 15.533694928282932 19 16.935631902084417 29.16354937361461 33.273538737819365 20.627279986481607 20 21.358267259973957 21.84559210896886 40.061230372650435 16.734910258406753 21 14.325256534718747 29.00576851075673 37.13363661364231 19.53533834088221 22 19.324676701644407 23.456600322718522 31.383614248652304 25.835108726984767 23 13.249020082104362 28.051727736894943 32.289214110817696 26.410038070182996 24 11.37983705033945 23.450570058745374 52.529298136913496 12.640294754001676 25 11.606005082651064 33.791810106324824 39.708095463717356 14.894089347306757 26 14.93789159440842 29.241081338983673 34.89945694490923 20.92157012169868 27 10.956128172929416 38.08860512042305 30.513135704132722 20.44213100251482 28 9.631458097948716 30.3989582884653 39.91935350268877 20.050230110897218 29 10.192471447362754 21.587218491042407 39.52818154407891 28.69212851751593 30 11.779292338582756 34.39496903691383 36.78374876992555 17.041989854577867 31 16.13208266100308 39.73599371792281 30.298431800033796 13.833491821040319 32 17.738187143609743 26.07790968520034 33.031731779159806 23.15217139203011 33 18.808260798976843 36.30371325100808 27.833909300853843 17.05411664916123 34 14.707217430776215 26.964292222616145 33.761923853007346 24.566566493600302 35 18.97518645775005 29.162422840784462 33.086865621200026 18.775525080265464 36 23.138056598334718 33.49102584067513 29.196351358963057 14.174566202027098 37 14.352624655827654 34.85254016586539 36.735374124866226 14.05946105344073 38 13.650993502556236 27.70647855777291 41.63532806955346 17.007199870117393 39 15.764766691737545 31.870210164639463 35.44171683603315 16.92330630758985 40 17.430643680979156 28.14483236197488 35.56755717982446 18.85696677722151 41 11.891813088323486 30.308504328868068 36.96280122328212 20.836881359526327 42 15.115022315290034 28.5577397775429 31.625288674037726 24.70194923312934 43 16.202722896117105 26.52660108478485 34.841009771015635 22.42966624808241 44 14.08391344251866 28.19936980428149 33.95774176554201 23.758974987657837 45 14.461898340352075 40.70295648601277 25.808800872068943 19.026344301566212 46 17.708168357018135 34.88289028564234 32.669717140859674 14.73922421647985 47 15.114160849008154 32.632409024190636 35.386582993992924 16.866847132808278 48 15.334166084072482 26.887356656980693 37.032314925566 20.746162333380823 49 18.177601214004792 27.989768431236765 36.28655019200758 17.54608016275086 50 16.90223351700236 31.428940628406522 33.84177515067376 17.82705070391735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3611.0 1 5067.0 2 6523.0 3 19570.0 4 32617.0 5 25294.0 6 17971.0 7 19113.0 8 20255.0 9 22093.5 10 23932.0 11 23616.5 12 23301.0 13 21911.0 14 20521.0 15 18650.5 16 16780.0 17 15041.0 18 13302.0 19 13037.5 20 12773.0 21 11856.5 22 10940.0 23 15168.5 24 19397.0 25 16527.0 26 13657.0 27 15255.0 28 16853.0 29 27591.5 30 38330.0 31 57012.5 32 75695.0 33 93238.0 34 110781.0 35 115588.0 36 120395.0 37 117651.5 38 114908.0 39 115577.5 40 116247.0 41 119018.0 42 121789.0 43 119898.5 44 118008.0 45 116120.5 46 114233.0 47 102486.5 48 90740.0 49 82878.0 50 75016.0 51 61241.0 52 47466.0 53 38419.0 54 29372.0 55 25152.0 56 20932.0 57 18315.5 58 15699.0 59 14233.0 60 12767.0 61 12201.5 62 11636.0 63 9881.5 64 8127.0 65 6750.0 66 5373.0 67 4216.0 68 3059.0 69 2431.5 70 1804.0 71 1864.5 72 1925.0 73 1486.0 74 1047.0 75 824.5 76 602.0 77 469.5 78 337.0 79 269.5 80 202.0 81 143.0 82 84.0 83 54.0 84 24.0 85 17.5 86 11.0 87 8.5 88 6.0 89 5.5 90 5.0 91 2.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1509055.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.173586324634968 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02075182588891 15.94131958397126 2 8.965742242097175 3.617423501707472 3 3.427961714071389 2.074628446690884 4 1.7543221231521064 1.4156387517050366 5 1.0441457690504614 1.0532082403720926 6 0.68975714184878 0.8348925146471909 7 0.4967889775622551 0.7015410726785509 8 0.3786098132606762 0.6110334200934542 9 0.29426024779114585 0.5342656059650842 >10 2.4494853311171423 10.857349925667844 >50 0.7392525863485494 10.584098680363748 >100 0.59259118314421 24.556691222232065 >500 0.10338965458424448 14.769772182200894 >1k 0.042611071697659875 11.203556396260108 >5k 0.0 0.0 >10k+ 3.303183852531773E-4 1.2445804554443642 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18677 1.237661980510982 No Hit TTTGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 2573 0.17050405717485448 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2329 0.15433499773036768 No Hit ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC 2259 0.14969633313563785 No Hit ATGTCTGGGAATGAAAGAGAGAGAGAGAGAAAGAAAAAAGAGAAAAAAGA 2136 0.1415455367763269 No Hit ACAATTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA 2106 0.13955753766429985 No Hit TATGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 1995 0.13220194094979973 No Hit TTCGTTGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT 1935 0.12822594272574558 No Hit AGGGGAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCG 1934 0.128159676088678 No Hit GATTAAGGGGAGTTTGTGAAATCTGGATTAAAAAATTACCTCTTCAGTGT 1887 0.12504514414650228 No Hit GGACTGGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT 1860 0.12325594494567793 No Hit CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG 1857 0.12305714503447521 TruSeq Adapter, Index 22 (95% over 21bp) TAAGTAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA 1740 0.11530394849756968 No Hit TTTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1687 0.11179181673298853 No Hit ATTAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1602 0.10615915258224518 No Hit GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA 1600 0.10602661930811004 No Hit CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC 1596 0.10576155275983977 TruSeq Adapter, Index 20 (95% over 21bp) TATACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1553 0.10291208736593431 No Hit GTTTTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1549 0.10264702081766404 No Hit AATACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1537 0.1018518211728532 No Hit GGAAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 1528 0.1012554214392451 No Hit TTTGCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA 1520 0.10072528834270454 No Hit TTAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 1518 0.1005927550685694 No Hit TTTTTAGGGAATCAATCTAATAGGAGAATTATTCATTACCATATCATTAT 1515 0.10039395515736668 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.11914741344748866 0.0 2 0.0 0.0 0.0 0.4673785912375626 0.0 3 0.0 0.0 0.0 0.6431839793778226 0.0 4 0.0 0.0 0.0 0.9507937086454769 0.0 5 0.0 0.0 0.0 1.5063731938199734 0.0 6 0.0 0.0 0.0 1.992041376888185 0.0 7 0.0 0.0 0.0 2.2612164566566495 0.0 8 0.0 0.0 0.0 2.814211542985511 0.0 9 0.0 0.0 0.0 3.048066505196961 0.0 10 0.0 0.0 0.0 3.573759737053984 0.0 11 0.0 0.0 0.0 4.12854402258367 0.0 12 0.0 0.0 0.0 4.6252787340421655 0.0 13 0.0 0.0 0.0 4.8009515889082905 0.0 14 0.0 0.0 0.0 4.872387023667129 0.0 15 0.0 0.0 0.0 4.991998303574091 0.0 16 0.0 0.0 0.0 5.250371921500542 0.0 17 0.0 0.0 0.0 5.579253241266885 0.0 18 0.0 0.0 0.0 6.06783715636607 0.0 19 0.0 0.0 0.0 6.2720046651712495 0.0 20 0.0 0.0 0.0 6.4756420408798885 0.0 21 0.0 0.0 0.0 6.757407781691191 0.0 22 0.0 0.0 0.0 7.022142996776128 0.0 23 0.0 0.0 0.0 7.34724711822962 0.0 24 0.0 0.0 0.0 7.580174347522124 0.0 25 0.0 0.0 0.0 7.757967734774412 0.0 26 0.0 0.0 0.0 7.953918180583213 0.0 27 0.0 0.0 0.0 8.127801836248514 0.0 28 0.0 0.0 0.0 8.317655751447099 0.0 29 0.0 0.0 0.0 8.52937765687798 0.0 30 0.0 0.0 0.0 8.841029651006757 0.0 31 0.0 0.0 0.0 9.114777128732882 0.0 32 0.0 0.0 0.0 9.32470983496294 0.0 33 0.0 0.0 0.0 9.522449479972565 0.0 34 0.0 0.0 0.0 9.720056591708056 0.0 35 0.0 0.0 0.0 10.06391417145167 0.0 36 0.0 0.0 0.0 10.279214475284201 0.0 37 0.0 0.0 0.0 10.517906902001585 0.0 38 0.0 0.0 0.0 10.71644174665602 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGGAA 40 8.314601E-9 44.000004 27 GCCCCTA 145 0.0 44.000004 13 AGCGTTG 20 7.8572653E-4 44.000004 1 AACGTAC 65 0.0 44.000004 16 CGGGTAC 40 8.314601E-9 44.000004 6 CTGTCGA 20 7.8572653E-4 44.000004 12 ATCTCGC 40 8.314601E-9 44.000004 13 GGTACGA 40 8.314601E-9 44.000004 8 CGAACTC 40 8.314601E-9 44.000004 13 TACCCGC 130 0.0 44.000004 40 TACCCGA 20 7.8572653E-4 44.000004 33 CAAACGT 65 0.0 44.000004 13 CGAACAT 80 0.0 44.000004 11 ATTAGTC 30 2.5282443E-6 44.000004 44 CTCCGTC 20 7.8572653E-4 44.000004 11 GTCGACA 30 2.5282443E-6 44.000004 18 GATCGAA 20 7.8572653E-4 44.000004 10 GAATGGA 20 7.8572653E-4 44.000004 3 CACGGCA 110 0.0 44.000004 4 CTATCCG 20 7.8572653E-4 44.000004 1 >>END_MODULE