FastQCFastQC Report
Thu 26 May 2016
SRR1546216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546216_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1539107
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192791.2526094676978274No Hit
TTTGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT25970.16873420756321686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23790.15457015009352826No Hit
ATGTCTGGGAATGAAAGAGAGAGAGAGAGAAAGAAAAAAGAGAAAAAAGA21750.1413157109934527No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC21350.1387168013659869No Hit
ACAATTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA20910.13585800077577453No Hit
TTCGTTGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT20100.1305952087801563No Hit
TATGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA19720.12812624463406377No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG19520.12682678982033088TruSeq Adapter, Index 22 (95% over 21bp)
GATTAAGGGGAGTTTGTGAAATCTGGATTAAAAAATTACCTCTTCAGTGT19190.1246826893776716No Hit
TTTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT17970.11675601501390091No Hit
TAAGTAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA17890.11623623308840776No Hit
AGGGGAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCG17670.11480683279330157No Hit
GGACTGGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT17600.11435202360849506No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA16800.10915420435356346No Hit
ATTAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16170.10506092169030483No Hit
TTTGCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA15740.1022670938407791No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG15620.10148742095253936TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC15400.10005802065743317TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTC302.528277E-644.0000049
CCTTATG302.528277E-644.0000041
GTCGACA302.528277E-644.00000418
TAGGTCA302.528277E-644.00000436
ACCGAAC600.044.00000411
GCAAACG302.528277E-644.00000410
ATTCGTC302.528277E-644.00000414
TAGCCGT302.528277E-644.00000444
AATGCAT302.528277E-644.00000418
TCGCTAG302.528277E-644.0000041
CTCATTA302.528277E-644.00000414
GCAATGC302.528277E-644.00000438
AGTAGGT302.528277E-644.00000443
TACAGCA302.528277E-644.00000425
AAGTATC207.857315E-444.034
AACGTCG207.857315E-444.020
CGGGTCA700.044.06
AGGTAGC1800.044.031
ATCTCGA454.802132E-1044.031
GTACCGG207.857315E-444.02
Produced by FastQC (version 0.11.5)