##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546216_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1539107
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89251624480949	31.0	31.0	34.0	30.0	34.0
2	32.298937630717035	33.0	31.0	34.0	31.0	34.0
3	32.346350188778295	34.0	31.0	34.0	30.0	34.0
4	36.03329658041969	37.0	35.0	37.0	35.0	37.0
5	35.96241846733203	37.0	35.0	37.0	35.0	37.0
6	36.041192067867925	37.0	35.0	37.0	35.0	37.0
7	36.19079245302633	37.0	35.0	37.0	35.0	37.0
8	36.100456953285246	37.0	35.0	37.0	35.0	37.0
9	37.908989433483185	39.0	38.0	39.0	35.0	39.0
10	37.603354412656174	39.0	37.0	39.0	35.0	39.0
11	37.47339658646215	39.0	37.0	39.0	35.0	39.0
12	36.88662581613884	39.0	35.0	39.0	33.0	39.0
13	36.60668101697932	39.0	35.0	39.0	33.0	39.0
14	37.80872350005555	40.0	35.0	41.0	33.0	41.0
15	38.010801068411745	40.0	35.0	41.0	33.0	41.0
16	38.11699121633519	40.0	35.0	41.0	34.0	41.0
17	38.02981014315444	40.0	35.0	41.0	34.0	41.0
18	37.86869204025451	39.0	36.0	41.0	34.0	41.0
19	37.628972514581505	38.0	36.0	41.0	34.0	41.0
20	37.27555848943576	38.0	35.0	41.0	34.0	41.0
21	37.200084854399336	38.0	35.0	41.0	33.0	41.0
22	37.1515300755568	38.0	35.0	41.0	33.0	41.0
23	37.09737529619448	38.0	35.0	41.0	33.0	41.0
24	37.00538559047551	38.0	35.0	41.0	33.0	41.0
25	37.00005782573921	38.0	35.0	41.0	33.0	41.0
26	36.93218080354387	38.0	35.0	41.0	33.0	41.0
27	36.88865231592086	38.0	35.0	41.0	33.0	41.0
28	36.81993324700622	38.0	35.0	41.0	33.0	41.0
29	36.721925766044855	38.0	35.0	41.0	33.0	41.0
30	36.53687885247744	38.0	35.0	41.0	32.0	41.0
31	36.20749174683761	38.0	35.0	41.0	31.0	41.0
32	35.85559093682246	38.0	35.0	41.0	30.0	41.0
33	35.40015736397795	38.0	35.0	41.0	27.0	41.0
34	34.982322866441386	38.0	35.0	41.0	23.0	41.0
35	34.68887283340275	38.0	35.0	41.0	21.0	41.0
36	34.39720175400411	38.0	34.0	40.0	18.0	41.0
37	34.30873811892221	38.0	34.0	40.0	18.0	41.0
38	34.33447187232596	38.0	34.0	41.0	18.0	41.0
39	34.26961348366293	38.0	34.0	41.0	18.0	41.0
40	34.123365042196546	38.0	34.0	41.0	18.0	41.0
41	34.00929565001004	38.0	34.0	40.0	16.0	41.0
42	33.92673998623877	38.0	34.0	40.0	15.0	41.0
43	33.82308052656508	37.0	34.0	40.0	15.0	41.0
44	33.635767363802515	37.0	34.0	40.0	14.0	41.0
45	33.53843754852652	37.0	34.0	40.0	13.0	41.0
46	33.44822939535718	37.0	34.0	40.0	12.0	41.0
47	33.43839772023647	37.0	33.0	40.0	12.0	41.0
48	33.434230368648834	37.0	33.0	40.0	12.0	41.0
49	33.28600740559298	36.0	33.0	40.0	10.0	41.0
50	33.137512206753655	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	10.0
15	19.0
16	59.0
17	199.0
18	486.0
19	1006.0
20	1792.0
21	3260.0
22	5440.0
23	8732.0
24	14054.0
25	24615.0
26	38502.0
27	45727.0
28	41939.0
29	34454.0
30	32928.0
31	34367.0
32	40687.0
33	53855.0
34	103036.0
35	219531.0
36	77299.0
37	98727.0
38	175437.0
39	482942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.85160485918133	22.46296066485306	29.265476669263414	20.4199578067022
2	33.96774883097796	24.743893699398416	30.91448482789046	10.37387264173316
3	24.41604124989361	23.106645606835652	42.5227095971885	9.954603546082241
4	23.205014336235234	26.215071466766116	38.8919678748781	11.687946322120554
5	19.45069446113883	30.279311314937818	39.009828426483665	11.260165797439683
6	15.360530489433158	40.11442999089732	35.97618619108353	8.548853328585992
7	77.60701497686645	4.605072941647332	16.095372186599114	1.6925398948871
8	78.90114202586305	3.779074489298015	15.384570403487215	1.935213081351719
9	76.27513876553093	4.349535152526758	16.65797114820477	2.71735493373755
10	38.26069272636665	29.681951937064806	23.42774089130905	8.629614445259492
11	33.310354640710486	19.055530252282654	33.001994013411675	14.632121093595183
12	34.792837664957666	18.885236698942958	36.360564924985724	9.961360711113652
13	16.38047257273211	39.65325347750351	29.84568324359515	14.120590706169226
14	9.145952815496258	40.42798843745107	32.93546192694855	17.490596820104127
15	8.014452536438338	26.289660173074385	52.850386620293456	12.845500670193822
16	9.753317995435015	19.882243404779526	46.49215421669838	23.872284383087074
17	9.27836726101564	23.978060004924934	34.400532256691704	32.343040477367715
18	13.109160051900226	27.498607959030792	43.79552558723987	15.596706401829113
19	16.99647912718219	29.068674237723563	33.349403257863166	20.585443377231083
20	21.334644050088787	21.86261254090846	40.069273936120105	16.733469472882653
21	14.334026159324855	29.058538490176446	37.19098152370173	19.416453826796968
22	19.282480035501106	23.369395370172445	31.4888438555604	25.85928073876605
23	13.226500821580306	28.09908602845676	32.35791923498496	26.316493914977972
24	11.372828529790327	23.42254307205412	52.512918205167026	12.69171019298853
25	11.568916261182622	33.67465679774051	39.83023922313394	14.926187717942938
26	14.88044690849954	29.220320614486194	35.00562339070643	20.89360908630784
27	11.00365341720881	38.07279156030088	30.531470521542687	20.392084500947625
28	9.604140582818479	30.393533393064942	39.98636871900394	20.01595730511264
29	10.170832827087395	21.576927400109284	39.62927853619014	28.622961236613182
30	11.760975682652344	34.444908638580685	36.728050746309385	17.066064932457586
31	16.18659391452316	39.5492321196642	30.347532692658795	13.916641273153848
32	17.7271625689442	26.080318002582015	33.10958887198876	23.082930556485024
33	18.88484686249884	36.19585902734508	27.894876704478637	17.024417405677447
34	14.735102887583515	26.952512073559536	33.693693810761694	24.61869122809525
35	18.98191613708469	29.061332318025972	33.107639689768156	18.849111855121183
36	23.29526147304898	33.33251034528464	29.12468074019545	14.247547441470932
37	14.4408413450137	34.71188163006211	36.760991925837516	14.086285099086679
38	13.732898362492016	27.736213271721848	41.441043410237235	17.089844955548898
39	15.80318977173127	31.80915946714556	35.468684113580146	16.918966647543023
40	17.511128206161107	28.111820685631344	35.442954908268234	18.934096199939315
41	11.927695735254273	30.21303911943744	36.93297477043506	20.926290374873222
42	15.227921125691715	28.48385459880307	31.614241245085623	24.67398303041959
43	16.304584411610108	26.465086572928325	34.76256036779769	22.46776864766387
44	14.172698844199916	28.070303104332577	33.9194091119071	23.83758893956041
45	14.542263793225551	40.59230449864759	25.833486560713453	19.031945147413403
46	17.8458677661787	34.7338424164142	32.61488642440064	14.805403393006463
47	15.154761819678553	32.54906903808507	35.262655552862796	17.033513589373577
48	15.365663335947403	26.873050411699772	37.032513009167005	20.72877324318582
49	18.192757228704696	27.892927522258038	36.366217553425464	17.548097695611805
50	16.99342540836992	31.292951042390165	33.841376850342435	17.872246698897477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3748.0
1	5243.5
2	6739.0
3	20082.0
4	33425.0
5	25748.0
6	18071.0
7	19287.0
8	20503.0
9	22335.5
10	24168.0
11	24072.5
12	23977.0
13	22416.5
14	20856.0
15	18931.0
16	17006.0
17	15316.5
18	13627.0
19	13246.0
20	12865.0
21	12024.5
22	11184.0
23	15509.0
24	19834.0
25	16963.0
26	14092.0
27	15601.0
28	17110.0
29	28249.5
30	39389.0
31	57998.5
32	76608.0
33	94415.5
34	112223.0
35	117432.0
36	122641.0
37	119007.5
38	115374.0
39	116788.0
40	118202.0
41	120870.0
42	123538.0
43	121814.0
44	120090.0
45	118825.0
46	117560.0
47	105615.5
48	93671.0
49	85807.0
50	77943.0
51	63566.5
52	49190.0
53	40004.0
54	30818.0
55	26166.5
56	21515.0
57	18772.5
58	16030.0
59	14439.5
60	12849.0
61	12217.5
62	11586.0
63	9890.5
64	8195.0
65	6818.0
66	5441.0
67	4244.0
68	3047.0
69	2397.0
70	1747.0
71	1856.5
72	1966.0
73	1494.0
74	1022.0
75	787.5
76	553.0
77	458.0
78	363.0
79	291.5
80	220.0
81	152.0
82	84.0
83	50.0
84	16.0
85	15.5
86	15.0
87	8.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1539107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.242389522491987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86652580915525	15.96446935714588
2	9.057327842328757	3.6668391643466114
3	3.431857008835051	2.08406959075
4	1.7548739284641093	1.4209136649134493
5	1.099697159177279	1.1130249126422176
6	0.7356887455448532	0.8935258892779445
7	0.49946473035245204	0.7077251737178533
8	0.3652794508425034	0.5915303142812733
9	0.2782990017676014	0.5070093117750426
>10	2.4509262522839896	10.846749755664119
>50	0.7309991352111499	10.55666515949798
>100	0.5837009798045528	24.383221808266697
>500	0.10045988086287182	14.309770235373815
>1k	0.04457705324461836	11.693882039855666
>5k	0.0	0.0
>10k+	3.230221249610026E-4	1.2606036224914863
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19279	1.2526094676978274	No Hit
TTTGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2597	0.16873420756321686	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2379	0.15457015009352826	No Hit
ATGTCTGGGAATGAAAGAGAGAGAGAGAGAAAGAAAAAAGAGAAAAAAGA	2175	0.1413157109934527	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2135	0.1387168013659869	No Hit
ACAATTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	2091	0.13585800077577453	No Hit
TTCGTTGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT	2010	0.1305952087801563	No Hit
TATGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1972	0.12812624463406377	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG	1952	0.12682678982033088	TruSeq Adapter, Index 22 (95% over 21bp)
GATTAAGGGGAGTTTGTGAAATCTGGATTAAAAAATTACCTCTTCAGTGT	1919	0.1246826893776716	No Hit
TTTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1797	0.11675601501390091	No Hit
TAAGTAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA	1789	0.11623623308840776	No Hit
AGGGGAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCG	1767	0.11480683279330157	No Hit
GGACTGGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT	1760	0.11435202360849506	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1680	0.10915420435356346	No Hit
ATTAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1617	0.10506092169030483	No Hit
TTTGCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	1574	0.1022670938407791	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTG	1562	0.10148742095253936	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC	1540	0.10005802065743317	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11402715990506183	0.0
2	0.0	0.0	0.0	0.46455509590951116	0.0
3	0.0	0.0	0.0	0.6380972862835398	0.0
4	0.0	0.0	0.0	0.9503562780235553	0.0
5	0.0	0.0	0.0	1.5219214778439705	0.0
6	0.0	0.0	0.0	2.014479824989426	0.0
7	0.0	0.0	0.0	2.2949671465336716	0.0
8	0.0	0.0	0.0	2.8764082029384572	0.0
9	0.0	0.0	0.0	3.11745707088591	0.0
10	0.0	0.0	0.0	3.663228092653727	0.0
11	0.0	0.0	0.0	4.250451722979624	0.0
12	0.0	0.0	0.0	4.767244902401197	0.0
13	0.0	0.0	0.0	4.951442622247836	0.0
14	0.0	0.0	0.0	5.022587773299712	0.0
15	0.0	0.0	0.0	5.150259208748969	0.0
16	0.0	0.0	0.0	5.423339637854938	0.0
17	0.0	0.0	0.0	5.760743080240685	0.0
18	0.0	0.0	0.0	6.267725375818575	0.0
19	0.0	0.0	0.0	6.4831749839354895	0.0
20	0.0	0.0	0.0	6.699794101384764	0.0
21	0.0	0.0	0.0	6.997109362766851	0.0
22	0.0	0.0	0.0	7.260963662695316	0.0
23	0.0	0.0	0.0	7.605644052037968	0.0
24	0.0	0.0	0.0	7.846043192578554	0.0
25	0.0	0.0	0.0	8.030305885165879	0.0
26	0.0	0.0	0.0	8.23613952766117	0.0
27	0.0	0.0	0.0	8.418258119805836	0.0
28	0.0	0.0	0.0	8.621038043488854	0.0
29	0.0	0.0	0.0	8.84402448952542	0.0
30	0.0	0.0	0.0	9.156153535784062	0.0
31	0.0	0.0	0.0	9.434171893182215	0.0
32	0.0	0.0	0.0	9.655339102479555	0.0
33	0.0	0.0	0.0	9.855520116535107	0.0
34	0.0	0.0	0.0	10.069215460653483	0.0
35	0.0	0.0	0.0	10.429749198723675	0.0
36	0.0	0.0	0.0	10.650786462539642	0.0
37	0.0	0.0	0.0	10.894174349151813	0.0
38	0.0	0.0	0.0	11.096369518168652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTC	30	2.528277E-6	44.000004	9
CCTTATG	30	2.528277E-6	44.000004	1
GTCGACA	30	2.528277E-6	44.000004	18
TAGGTCA	30	2.528277E-6	44.000004	36
ACCGAAC	60	0.0	44.000004	11
GCAAACG	30	2.528277E-6	44.000004	10
ATTCGTC	30	2.528277E-6	44.000004	14
TAGCCGT	30	2.528277E-6	44.000004	44
AATGCAT	30	2.528277E-6	44.000004	18
TCGCTAG	30	2.528277E-6	44.000004	1
CTCATTA	30	2.528277E-6	44.000004	14
GCAATGC	30	2.528277E-6	44.000004	38
AGTAGGT	30	2.528277E-6	44.000004	43
TACAGCA	30	2.528277E-6	44.000004	25
AAGTATC	20	7.857315E-4	44.0	34
AACGTCG	20	7.857315E-4	44.0	20
CGGGTCA	70	0.0	44.0	6
AGGTAGC	180	0.0	44.0	31
ATCTCGA	45	4.802132E-10	44.0	31
GTACCGG	20	7.857315E-4	44.0	2
>>END_MODULE