FastQCFastQC Report
Thu 26 May 2016
SRR1546215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546215_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1244031
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179261.4409608763768749No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC31120.250154537949617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30360.24404536542899655No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21790.17515640687410522No Hit
TACGAAGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG19100.153533151505067No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18990.15264892916655612No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC17780.1429224834429367No Hit
AGCTAAGGGACATAGTGAAAATTTCACATGGTCTGATTAGCCAAAAAAGA17300.13906405869307115No Hit
TAATCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17180.13809945250560476No Hit
TTCGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17030.13689369477127178No Hit
AAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16590.13335680541722836No Hit
AATATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16450.1322314315318509No Hit
TTTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA16400.13182951228707324No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG16150.12981991606318494No Hit
TGGCATGGGAATACCAAGCTAGTATTTTATATATTGTATCTTAGCTAATA15520.12475573357898638No Hit
TAGTTAGGGGATACATCAACATGATTTATCACAATTATCTCATCAATAAT15380.12363035969360892No Hit
TGGTAACGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT15190.12210306656345381No Hit
GAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15050.12097769267807636No Hit
ACTATTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14570.1171192679282108No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA14550.11695850023029973No Hit
AGTGAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14110.1134216108762563No Hit
ATTATGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14040.11285892393356758No Hit
TTAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT14000.11253738853774543No Hit
GGTATGGGGGAAATAAGATACTCAATGATACAGCCAAATTTAAACAATAT14000.11253738853774543No Hit
TTGTAAGGGGAAAGTTTTGTTGAAGTAGTCTAATATTTTTTATGTAAAGA13790.11084932770967927No Hit
TGGAGAGGGATCTCAATACTAATCAGCTTACAAATTCTCCTAATCATAAC13740.11044740846490159No Hit
TGGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13660.10980433767325734No Hit
GGTATTGGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA13430.1079555091472801No Hit
GAAACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA13250.1065085998660805No Hit
TATTCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13060.1049813067359254No Hit
TACAAAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA12930.10393631669950347No Hit
TTAGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12880.1035343974547258No Hit
TTAGTAGGGGAGAAAATAGTTTTCATTGCTAAAAAATTGTTAACATATAG12870.10345401360577026No Hit
TTGTATGGGATGCCACAACTAGATACATCACATGATTTATCACAATTATC12810.10297171051203709No Hit
GCACAAGGGGGAAATTCATCGACAATAAACAAGAAATAGAACTTTTAACA12730.10232863972039281No Hit
TCTCATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12630.10152480123083749No Hit
AGAAAGAGGGCAGACTCAGTATTCACAGTTACTTACCATACAAAAGTTCA12540.1008013465902377No Hit
TTAGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT12530.10072096274128219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA551.8189894E-1244.00000439
TGATAGT600.044.00000432
TAGCGAG600.044.0000041
GTGCTAG551.8189894E-1244.0000041
TGCGAAG650.044.0000041
TATAGCG600.044.0000041
ATAACGG551.8189894E-1244.0000042
GCGTGGA551.8189894E-1244.00000443
TGTACGT551.8189894E-1244.00000410
TAATGCG302.527886E-644.0000041
GCGTAAG551.8189894E-1244.0000041
GTACGTA551.8189894E-1244.00000411
TACGTAT551.8189894E-1244.00000412
TAACTAT302.527886E-644.00000427
GTGTTCG207.856733E-444.020
CTAGACG351.4462057E-744.01
CTATGCG700.044.01
TCGTTAG454.802132E-1044.01
ACGCCTA408.312782E-944.043
CGAGTCT207.856733E-444.017
Produced by FastQC (version 0.11.5)