##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546215_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1244031
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90522824591992	33.0	31.0	34.0	30.0	34.0
2	32.27040403334001	33.0	31.0	34.0	30.0	34.0
3	32.13441706838495	33.0	31.0	34.0	30.0	34.0
4	35.91362675045879	37.0	35.0	37.0	35.0	37.0
5	35.88286385146351	37.0	35.0	37.0	35.0	37.0
6	35.9578250059685	37.0	35.0	37.0	35.0	37.0
7	36.17939102803708	37.0	35.0	37.0	35.0	37.0
8	36.08059043544735	37.0	35.0	37.0	35.0	37.0
9	37.87031352112608	39.0	38.0	39.0	35.0	39.0
10	37.53563295448426	39.0	37.0	39.0	35.0	39.0
11	37.34082269654052	39.0	37.0	39.0	34.0	39.0
12	36.58141959484932	39.0	35.0	39.0	33.0	39.0
13	36.306130635008294	39.0	35.0	39.0	33.0	39.0
14	37.42902628632245	40.0	35.0	41.0	33.0	41.0
15	37.66541428629994	40.0	35.0	41.0	33.0	41.0
16	37.773905151881266	40.0	35.0	41.0	34.0	41.0
17	37.7172891993849	40.0	35.0	41.0	34.0	41.0
18	37.49943932265354	39.0	35.0	41.0	34.0	41.0
19	37.19694123377955	37.0	35.0	41.0	34.0	41.0
20	36.846280357965355	37.0	35.0	41.0	33.0	41.0
21	36.72966590060859	36.0	35.0	41.0	33.0	41.0
22	36.63458145335606	36.0	35.0	41.0	33.0	41.0
23	36.598502770429356	36.0	35.0	41.0	33.0	41.0
24	36.53471738244465	36.0	35.0	40.0	33.0	41.0
25	36.498987565422404	36.0	35.0	40.0	33.0	41.0
26	36.408421494319676	36.0	35.0	40.0	33.0	41.0
27	36.313973687150884	36.0	35.0	40.0	32.0	41.0
28	36.254438193260455	36.0	35.0	41.0	32.0	41.0
29	36.11776796558928	36.0	35.0	40.0	32.0	41.0
30	35.89619068978185	36.0	35.0	40.0	31.0	41.0
31	35.499583209743165	36.0	35.0	40.0	30.0	41.0
32	35.049155527474795	36.0	35.0	40.0	27.0	41.0
33	34.51850154859485	36.0	34.0	40.0	23.0	41.0
34	33.945902473491415	36.0	34.0	40.0	18.0	41.0
35	33.636495392799695	36.0	34.0	40.0	15.0	41.0
36	33.41920739917253	36.0	33.0	40.0	15.0	41.0
37	33.34966411608714	35.0	33.0	40.0	15.0	41.0
38	33.33900602155413	35.0	33.0	40.0	15.0	41.0
39	33.28216901347314	35.0	33.0	40.0	15.0	41.0
40	33.076635550078734	35.0	33.0	40.0	12.0	41.0
41	32.99614880979654	35.0	33.0	40.0	10.0	41.0
42	32.869399556763454	35.0	33.0	40.0	10.0	41.0
43	32.71928834570843	35.0	33.0	40.0	10.0	41.0
44	32.52866769397226	35.0	32.0	40.0	10.0	41.0
45	32.47035323074746	35.0	32.0	40.0	10.0	41.0
46	32.403577563581614	35.0	32.0	40.0	10.0	41.0
47	32.39563483546632	35.0	32.0	40.0	10.0	41.0
48	32.35774510442264	35.0	32.0	40.0	10.0	41.0
49	32.256852924083084	35.0	32.0	40.0	10.0	41.0
50	32.116242280136106	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	8.0
14	13.0
15	29.0
16	75.0
17	223.0
18	615.0
19	1233.0
20	2190.0
21	3491.0
22	5775.0
23	8926.0
24	14108.0
25	24033.0
26	36548.0
27	43686.0
28	40563.0
29	33656.0
30	30919.0
31	31064.0
32	36744.0
33	47477.0
34	91730.0
35	206172.0
36	59583.0
37	74047.0
38	127325.0
39	323792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.21659910404162	21.629444925407807	30.858796927086225	23.29515904346435
2	36.23567258372179	24.627360572204392	28.298892873248338	10.838073970825485
3	23.218472851560772	23.779230581874565	41.93263672689829	11.069659839666375
4	22.428058464781024	26.339616938806188	40.48612936494348	10.746195231469311
5	18.439331495758545	28.798800029902793	42.04461142849334	10.71725704584532
6	15.813512685777123	39.6001385817556	36.21589815687873	8.370450575588551
7	75.77608596570343	4.078194192909984	18.665210111323592	1.4805097300629968
8	76.17077066407509	3.4351234012657237	18.026721199069797	2.3673847355893862
9	73.54245995477605	4.274732703606261	19.86284907691207	2.3199582647056225
10	43.35486816646852	23.29339059878733	24.751151699595912	8.60058953514824
11	36.94457774766063	19.33392335078467	31.532735116729405	12.188763784825298
12	36.27875832676196	17.85373515611749	35.418731526786715	10.448774990333842
13	15.102356774067527	40.584599579914006	31.7861853924862	12.526858253532266
14	9.568652228119717	39.73076233630834	35.88343055759864	14.817154877973298
15	7.91596029359397	22.44140218370764	57.75748353537814	11.885153987320251
16	8.739733977690268	18.04553101972539	53.585561774586	19.629173227998336
17	9.391646992719634	19.872012835693003	35.9946818045531	34.74165836703426
18	12.927410972877684	25.736657687790736	45.315992929436646	16.01993840989493
19	18.18716736158504	24.569725352503273	36.79377764702005	20.449329638891637
20	21.380978448286257	19.907060193837616	40.87414220385183	17.8378191540243
21	14.506230150213298	27.88113801022643	39.272574397261806	18.340057442298463
22	18.677589224062743	21.354934081224663	33.03486810216144	26.932608592551148
23	12.58819113028534	26.362044032664784	34.506857144235155	26.542907692814726
24	11.701557276305815	21.27873019241482	53.342802550740295	13.67690998053907
25	11.394008670201949	26.305694954546954	44.892128893894125	17.408167481356976
26	12.872910723285836	29.54106449115818	37.60878949158019	19.97723529397579
27	11.422625320430118	36.6273830796821	34.4181937588372	17.531797841050583
28	10.445479252526665	28.125746062598118	43.48484885022962	17.9439258346456
29	10.50817865471198	22.73544630318698	41.25861815340615	25.497756888694894
30	12.747833454311024	32.562452221849775	38.76004697632133	15.929667347517867
31	19.237141196642206	33.357689639566864	32.86164090766227	14.543528256128665
32	20.105527916908823	24.691024580577174	37.110891931149624	18.09255557136438
33	20.99280484167999	31.833451095672054	30.780663825901446	16.393080236746513
34	15.662471433589678	25.592850982009292	34.5024360325426	24.242241551858434
35	17.221596567931186	27.71426113979475	34.2583906671136	20.805751625160465
36	25.154115934409994	28.151147358868066	31.798805656772217	14.89593104994972
37	13.885104149333898	32.1719474836238	37.763448016970635	16.179500350071663
38	15.306531750414578	30.17312269549553	35.38231764320985	19.138027910880034
39	15.478794338726285	31.73184591059226	34.3274403933664	18.46191935731505
40	18.64656105836591	25.09865107863068	34.05493914540715	22.199848717596264
41	12.954098410730921	27.43259613305456	34.78683409014727	24.826471366067242
42	17.454790113751184	27.4614539348296	30.327057766245375	24.756698185173843
43	17.406961723622643	26.364134012737622	34.235240118614406	21.993664145025324
44	14.602128082017249	28.743495941821383	33.748274761641795	22.906101214519573
45	14.784760186844217	38.4654401698993	27.13847162972627	19.611328013530212
46	20.69450037820601	31.01450044251309	32.54565199741807	15.745347181862831
47	16.735193897901258	31.106459565718218	32.21342554968486	19.94492098669567
48	17.178269673344154	27.65678668779154	34.396650887317115	20.768292751547186
49	19.310531650738607	25.466487571451196	36.130289357741084	19.092691420069112
50	16.391794095163224	30.363793185218054	32.82458395329377	20.419828766324954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3734.0
1	4727.5
2	5721.0
3	18494.0
4	31267.0
5	23763.5
6	16260.0
7	18029.5
8	19799.0
9	21845.0
10	23891.0
11	23874.0
12	23857.0
13	22855.0
14	21853.0
15	19531.0
16	17209.0
17	15713.5
18	14218.0
19	12466.5
20	10715.0
21	11257.5
22	11800.0
23	11264.0
24	10728.0
25	12948.5
26	15169.0
27	16870.5
28	18572.0
29	22092.0
30	25612.0
31	34073.5
32	42535.0
33	56500.5
34	70466.0
35	66889.5
36	63313.0
37	66294.5
38	69276.0
39	75804.5
40	82333.0
41	92400.5
42	102468.0
43	102964.0
44	103460.0
45	104425.5
46	105391.0
47	96224.5
48	87058.0
49	79588.5
50	72119.0
51	60454.0
52	48789.0
53	39668.5
54	30548.0
55	25530.0
56	20512.0
57	18788.0
58	17064.0
59	16061.5
60	15059.0
61	13569.5
62	12080.0
63	11866.5
64	11653.0
65	8934.0
66	6215.0
67	5833.0
68	5451.0
69	4295.0
70	3139.0
71	2560.5
72	1982.0
73	1708.0
74	1434.0
75	1021.5
76	609.0
77	473.0
78	337.0
79	246.0
80	155.0
81	124.5
82	94.0
83	71.5
84	49.0
85	34.5
86	20.0
87	13.0
88	6.0
89	6.0
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1244031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.715609549404558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96591483672007	18.964404135285903
2	8.618458866623367	4.08784010796887
3	3.2484761621891143	2.3111877687907567
4	1.677610963425391	1.5914226633758792
5	1.03292642520802	1.2248239896747817
6	0.6622981443881153	0.9424082518562223
7	0.48052192257926585	0.7977109207073353
8	0.3531704645829475	0.6700522273944794
9	0.28145529448519685	0.6007395482661245
>10	2.3618877565477754	12.288090782550027
>50	0.7086218198786461	11.999070581205018
>100	0.5151752177644576	24.065702235209617
>500	0.06959983074433715	11.405177186206794
>1k	0.02354111922234933	7.6009431891020895
>5k	0.0	0.0
>10k+	3.411756409036135E-4	1.450426412406128
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17926	1.4409608763768749	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3112	0.250154537949617	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3036	0.24404536542899655	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2179	0.17515640687410522	No Hit
TACGAAGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG	1910	0.153533151505067	No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1899	0.15264892916655612	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	1778	0.1429224834429367	No Hit
AGCTAAGGGACATAGTGAAAATTTCACATGGTCTGATTAGCCAAAAAAGA	1730	0.13906405869307115	No Hit
TAATCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1718	0.13809945250560476	No Hit
TTCGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1703	0.13689369477127178	No Hit
AAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1659	0.13335680541722836	No Hit
AATATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1645	0.1322314315318509	No Hit
TTTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1640	0.13182951228707324	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	1615	0.12981991606318494	No Hit
TGGCATGGGAATACCAAGCTAGTATTTTATATATTGTATCTTAGCTAATA	1552	0.12475573357898638	No Hit
TAGTTAGGGGATACATCAACATGATTTATCACAATTATCTCATCAATAAT	1538	0.12363035969360892	No Hit
TGGTAACGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT	1519	0.12210306656345381	No Hit
GAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1505	0.12097769267807636	No Hit
ACTATTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1457	0.1171192679282108	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1455	0.11695850023029973	No Hit
AGTGAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1411	0.1134216108762563	No Hit
ATTATGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1404	0.11285892393356758	No Hit
TTAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1400	0.11253738853774543	No Hit
GGTATGGGGGAAATAAGATACTCAATGATACAGCCAAATTTAAACAATAT	1400	0.11253738853774543	No Hit
TTGTAAGGGGAAAGTTTTGTTGAAGTAGTCTAATATTTTTTATGTAAAGA	1379	0.11084932770967927	No Hit
TGGAGAGGGATCTCAATACTAATCAGCTTACAAATTCTCCTAATCATAAC	1374	0.11044740846490159	No Hit
TGGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1366	0.10980433767325734	No Hit
GGTATTGGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA	1343	0.1079555091472801	No Hit
GAAACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1325	0.1065085998660805	No Hit
TATTCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1306	0.1049813067359254	No Hit
TACAAAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA	1293	0.10393631669950347	No Hit
TTAGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1288	0.1035343974547258	No Hit
TTAGTAGGGGAGAAAATAGTTTTCATTGCTAAAAAATTGTTAACATATAG	1287	0.10345401360577026	No Hit
TTGTATGGGATGCCACAACTAGATACATCACATGATTTATCACAATTATC	1281	0.10297171051203709	No Hit
GCACAAGGGGGAAATTCATCGACAATAAACAAGAAATAGAACTTTTAACA	1273	0.10232863972039281	No Hit
TCTCATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1263	0.10152480123083749	No Hit
AGAAAGAGGGCAGACTCAGTATTCACAGTTACTTACCATACAAAAGTTCA	1254	0.1008013465902377	No Hit
TTAGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1253	0.10072096274128219	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0693712616486245	0.0
2	0.0	0.0	0.0	0.3683187959142497	0.0
3	0.0	0.0	0.0	0.5574619925066177	0.0
4	0.0	0.0	0.0	0.8937880165365654	0.0
5	0.0	0.0	0.0	1.4903969434845272	0.0
6	0.0	0.0	0.0	2.07350138380796	0.0
7	0.0	0.0	0.0	2.401708639093399	0.0
8	0.0	0.0	0.0	3.172589750576955	0.0
9	0.0	0.0	0.0	3.498063954997906	0.0
10	0.0	0.0	0.0	4.062037039269922	0.0
11	0.0	0.0	0.0	4.783803619041648	0.0
12	0.0	0.0	0.0	5.341989066188865	0.0
13	0.0	0.0	0.0	5.565456166285245	0.0
14	0.0	0.0	0.0	5.640534681209712	0.0
15	0.0	0.0	0.0	5.76472772784601	0.0
16	0.0	0.0	0.0	6.0867454267618735	0.0
17	0.0	0.0	0.0	6.445096625405637	0.0
18	0.0	0.0	0.0	7.031094884291469	0.0
19	0.0	0.0	0.0	7.235511012185388	0.0
20	0.0	0.0	0.0	7.48269134772365	0.0
21	0.0	0.0	0.0	7.805030582035335	0.0
22	0.0	0.0	0.0	8.077853365390412	0.0
23	0.0	0.0	0.0	8.428889633779223	0.0
24	0.0	0.0	0.0	8.663288937333554	0.0
25	0.0	0.0	0.0	8.84278607205126	0.0
26	0.0	0.0	0.0	9.042379169007846	0.0
27	0.0	0.0	0.0	9.22959315322528	0.0
28	8.038384895553245E-5	0.0	0.0	9.415360228161516	0.0
29	8.038384895553245E-5	0.0	0.0	9.623715164654257	0.0
30	8.038384895553245E-5	0.0	0.0	9.946215166663853	0.0
31	8.038384895553245E-5	0.0	0.0	10.248458438736655	0.0
32	8.038384895553245E-5	0.0	0.0	10.472809761171547	0.0
33	8.038384895553245E-5	0.0	0.0	10.693383042705527	0.0
34	8.038384895553245E-5	0.0	0.0	10.913393637296819	0.0
35	8.038384895553245E-5	0.0	0.0	11.258079581618142	0.0
36	1.607676979110649E-4	0.0	0.0	11.4884596927247	0.0
37	1.607676979110649E-4	0.0	0.0	11.742151120028359	0.0
38	1.607676979110649E-4	0.0	0.0	11.967386664801761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	55	1.8189894E-12	44.000004	39
TGATAGT	60	0.0	44.000004	32
TAGCGAG	60	0.0	44.000004	1
GTGCTAG	55	1.8189894E-12	44.000004	1
TGCGAAG	65	0.0	44.000004	1
TATAGCG	60	0.0	44.000004	1
ATAACGG	55	1.8189894E-12	44.000004	2
GCGTGGA	55	1.8189894E-12	44.000004	43
TGTACGT	55	1.8189894E-12	44.000004	10
TAATGCG	30	2.527886E-6	44.000004	1
GCGTAAG	55	1.8189894E-12	44.000004	1
GTACGTA	55	1.8189894E-12	44.000004	11
TACGTAT	55	1.8189894E-12	44.000004	12
TAACTAT	30	2.527886E-6	44.000004	27
GTGTTCG	20	7.856733E-4	44.0	20
CTAGACG	35	1.4462057E-7	44.0	1
CTATGCG	70	0.0	44.0	1
TCGTTAG	45	4.802132E-10	44.0	1
ACGCCTA	40	8.312782E-9	44.0	43
CGAGTCT	20	7.856733E-4	44.0	17
>>END_MODULE