FastQCFastQC Report
Thu 26 May 2016
SRR1546214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546214_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1264747
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183091.44764130691751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31190.2466105869395223No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC30960.24479204141223504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22450.1775058569026058No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT19090.15093927876484386No Hit
TACGAAGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG18910.14951606922174948No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC18270.14445576862408055No Hit
AGCTAAGGGACATAGTGAAAATTTCACATGGTCTGATTAGCCAAAAAAGA18060.14279535749047043No Hit
AATATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17870.14129308075053745No Hit
TAATCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17550.138762930451703No Hit
AAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT17460.1380513256801558No Hit
TTCGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17230.13623278015286852No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG16830.13307009227932542No Hit
GAGATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16010.12658658213856208No Hit
TTTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA15510.12263322229663323No Hit
TAGTTAGGGGATACATCAACATGATTTATCACAATTATCTCATCAATAAT15350.121368147147216No Hit
TGGCATGGGAATACCAAGCTAGTATTTTATATATTGTATCTTAGCTAATA15230.12041934078515308No Hit
TGGTAACGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT15170.11994493760412162No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA14980.11844266086418864No Hit
ATTATGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14810.11709851851793283No Hit
AGTGAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14590.11535904018748415No Hit
GGTATGGGGGAAATAAGATACTCAATGATACAGCCAAATTTAAACAATAT14360.11354049466019688No Hit
ACTATTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14320.11322422587284255No Hit
TGGAGAGGGATCTCAATACTAATCAGCTTACAAATTCTCCTAATCATAAC14140.11180101632974816No Hit
TTAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT14030.1109312771645238No Hit
GGTATTGGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACA13810.10919179883407512No Hit
TTAGTAGGGGAGAAAATAGTTTTCATTGCTAAAAAATTGTTAACATATAG13680.10816392527517361No Hit
TTGTAAGGGGAAAGTTTTGTTGAAGTAGTCTAATATTTTTTATGTAAAGA13520.10689885012575638No Hit
GAAACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA13500.10674071573207923No Hit
TTGTATGGGATGCCACAACTAGATACATCACATGATTTATCACAATTATC13480.10658258133840205No Hit
TGGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13310.10523843899214624No Hit
TTAGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC13270.10492217020479196No Hit
TACAAAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA13180.10421056543324476No Hit
GATTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA12970.10255015429963463No Hit
TATTCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT12940.1023129527091189No Hit
TCTCATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12870.10175948233124886No Hit
AGAAAGAGGGCAGACTCAGTATTCACAGTTACTTACCATACAAAAGTTCA12780.10104787755970167No Hit
TTAGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT12720.1005734743786702No Hit
AGAAGAGGGTAGCTCTACCCCCTTCAATCAATCTAATAGGAGAATTATTC12670.10017813839447731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG1300.044.0000042
TACGTAG1300.044.0000041
GCGAGAC351.4462421E-744.021
TCACGAG600.044.044
CTTCGCA207.8567816E-444.024
AGCGTGA207.8567816E-444.035
CTCACGT207.8567816E-444.044
ACACGTG302.5279205E-644.042
CGAACTC254.4430948E-544.013
ACGCCTA408.312782E-944.043
CGTATAT850.044.014
GTCGACG207.8567816E-444.01
GTCGACC254.4430948E-544.09
GTTTCGA302.5279205E-644.01
CGAAACA207.8567816E-444.042
CGACGGC254.4430948E-544.020
ACGTTCA600.044.040
CGTGCGT207.8567816E-444.023
CTATCAG254.4430948E-544.01
AGCCCAA207.8567816E-444.039
Produced by FastQC (version 0.11.5)