FastQCFastQC Report
Thu 26 May 2016
SRR1546213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546213_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2261105
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308411.3639791164054744No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.10437374646467103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCA207.858098E-444.00000418
TCGACTA408.3182385E-944.00000444
ACGGGTC1850.044.05
TACGCGG254.444212E-544.02
ACTACGG351.4469151E-744.02
TTACGAA1650.044.026
TACGTCG454.802132E-1044.042
CGTTTTT184600.042.7724841
CTATGCG1350.042.370371
ATAACGG1250.042.242
CGTAAGA1200.042.16666422
ACGGGCC1850.041.6216245
TACGATC2700.041.55555318
ACGTAGG1650.041.3333322
TAGGGAC22700.041.092515
GCGCGTC750.041.06666638
TGCGTAG750.041.0666661
TCGATAG2150.040.9302331
CGACGGT1400.040.85714328
GTTTACG1400.040.8571431
Produced by FastQC (version 0.11.5)