##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546213_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2261105
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851152865523716	31.0	31.0	34.0	30.0	34.0
2	32.226307933510384	33.0	31.0	34.0	30.0	34.0
3	32.18297867635515	33.0	31.0	34.0	30.0	34.0
4	35.92921912073964	37.0	35.0	37.0	35.0	37.0
5	35.90209831033941	37.0	35.0	37.0	35.0	37.0
6	35.95954986610529	37.0	35.0	37.0	35.0	37.0
7	36.210680176285486	37.0	35.0	37.0	35.0	37.0
8	36.11238575829075	37.0	35.0	37.0	35.0	37.0
9	37.86576209419731	39.0	38.0	39.0	35.0	39.0
10	37.39449472713562	39.0	37.0	39.0	34.0	39.0
11	37.26502307500094	39.0	37.0	39.0	34.0	39.0
12	37.014145738477424	39.0	35.0	39.0	33.0	39.0
13	36.89696984439024	39.0	35.0	39.0	33.0	39.0
14	38.04217539654284	40.0	37.0	41.0	33.0	41.0
15	38.18598163287419	40.0	37.0	41.0	33.0	41.0
16	38.23317139186371	40.0	36.0	41.0	34.0	41.0
17	38.13737884795266	40.0	36.0	41.0	33.0	41.0
18	37.972144593019785	39.0	36.0	41.0	33.0	41.0
19	37.81163944177736	39.0	36.0	41.0	34.0	41.0
20	37.619810225531324	39.0	35.0	41.0	33.0	41.0
21	37.530799763832285	39.0	35.0	41.0	33.0	41.0
22	37.44661658790724	39.0	35.0	41.0	33.0	41.0
23	37.41595237726687	39.0	35.0	41.0	33.0	41.0
24	37.35770607733829	39.0	35.0	41.0	33.0	41.0
25	37.335302871825945	39.0	35.0	41.0	33.0	41.0
26	37.30305049964508	39.0	35.0	41.0	33.0	41.0
27	37.24340576841854	39.0	35.0	41.0	33.0	41.0
28	37.177859497900364	39.0	35.0	41.0	33.0	41.0
29	37.12024076723549	39.0	35.0	41.0	33.0	41.0
30	36.941439694308755	39.0	35.0	41.0	32.0	41.0
31	36.71763098131223	39.0	35.0	41.0	31.0	41.0
32	36.46285908880835	39.0	35.0	41.0	31.0	41.0
33	36.160010702731626	39.0	35.0	41.0	30.0	41.0
34	35.7708425747588	39.0	35.0	41.0	27.0	41.0
35	35.584475731998296	39.0	35.0	41.0	25.0	41.0
36	35.45668732765617	39.0	35.0	41.0	24.0	41.0
37	35.41451370015988	39.0	35.0	41.0	24.0	41.0
38	35.32458244973144	39.0	35.0	41.0	23.0	41.0
39	35.26010954820762	39.0	35.0	41.0	23.0	41.0
40	35.143751395888295	39.0	35.0	41.0	23.0	41.0
41	35.04068055220788	39.0	35.0	41.0	23.0	41.0
42	34.969217705502395	39.0	35.0	41.0	22.0	41.0
43	34.86838382118477	38.0	35.0	41.0	22.0	41.0
44	34.77246832853848	38.0	34.0	41.0	21.0	41.0
45	34.72563768599866	38.0	34.0	40.0	21.0	41.0
46	34.6691776808242	38.0	34.0	40.0	21.0	41.0
47	34.64345264815212	38.0	34.0	40.0	21.0	41.0
48	34.563131300846266	38.0	34.0	40.0	20.0	41.0
49	34.49248884947846	38.0	34.0	40.0	20.0	41.0
50	34.371977418120785	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	6.0
13	5.0
14	9.0
15	29.0
16	93.0
17	279.0
18	642.0
19	1338.0
20	2400.0
21	3970.0
22	6444.0
23	10056.0
24	15894.0
25	26569.0
26	41277.0
27	52430.0
28	52303.0
29	48231.0
30	47389.0
31	51940.0
32	61186.0
33	78202.0
34	134997.0
35	223506.0
36	133104.0
37	173228.0
38	312606.0
39	782966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.449983525754003	23.80897835350415	29.473200050417823	18.26783807032402
2	34.11292266391875	23.43898226751964	31.386379668347995	11.061715400213611
3	24.71769334020313	24.490724667806226	39.705099940073545	11.086482051917093
4	23.16562034934247	26.361093359220384	38.22936130785612	12.243924983581037
5	19.806422081239038	30.74974404107726	37.53412601360839	11.909707864075308
6	16.971923019939368	41.35278989697515	33.531525515179524	8.143761567905958
7	83.11201823886994	2.708012232956895	12.976708290857788	1.2032612373153835
8	83.66878141439695	1.8962852233752967	12.479163948600352	1.955769413627408
9	79.25664663958551	3.5881571178693603	14.512417601128652	2.642778641416476
10	35.09598183189193	27.894812492122213	22.905880089602206	14.103325586383649
11	25.480240855687818	22.673560051390805	34.07497661541591	17.771222477505468
12	27.478600065012458	20.687539941754142	35.70028813345687	16.133571859776524
13	20.21776963033561	27.412039688559354	36.364211303765195	16.00597937733984
14	14.483405237704575	31.08241324485152	37.302778950999624	17.131402566444283
15	15.022345269237828	25.888182990175157	44.38750080159922	14.701970938987795
16	17.107741568834705	22.8058847333494	42.686385638880104	17.399988058935786
17	16.899569016034196	24.04956868433797	36.71271347416418	22.338148825463655
18	17.693295976967015	24.88172818157494	40.41324927413809	17.01172656731996
19	19.094778880237758	25.980217636951846	36.36659951660803	18.558403966202366
20	20.276413523476354	25.676649248929174	37.432848098606655	16.614089128987818
21	18.53124910165605	25.945500098403212	39.26350169496773	16.25974910497301
22	17.979793065779784	22.894292834698078	37.868962299406704	21.25695180011543
23	15.745398820488212	25.633617191594375	37.97771443608325	20.64326955183417
24	14.934467881854227	24.685850502298653	44.15805546403197	16.22162615181515
25	16.055203097600508	27.041601340937284	39.015835177932914	17.88736038352929
26	15.390616534835843	28.717153781005305	36.55398577244312	19.338243911715733
27	14.809971230880475	29.384570818250367	37.62726631447898	18.178191636390174
28	14.244053239455932	27.439327231596938	40.80580070363827	17.510818825308863
29	15.140207995648144	24.392542584267428	39.54818551106649	20.919063909017936
30	16.0651097582819	28.17843488029083	38.15908593364748	17.59736942777978
31	18.29640817211054	29.415308002060943	34.984045411424944	17.304238414403578
32	18.831588979724515	26.363570024390732	36.52125841126352	18.283582584621236
33	19.064572410392262	27.52946015333211	34.76017257049098	18.645794865784648
34	15.765698629652316	28.090955528381034	35.85105512570181	20.292290716264834
35	16.097925571789016	26.65484353888917	36.46522386178439	20.78200702753742
36	20.382777447310055	28.068046375555316	34.128136464250886	17.421039712883747
37	16.547484526370955	29.088918913540063	36.27385725121125	18.08973930887774
38	16.464250886181755	28.146415137731328	36.20216663976242	19.187167336324496
39	16.861844098350144	28.68133058836277	35.10535777860825	19.35146753467884
40	18.223169644930245	26.41947189537859	35.52152597955425	19.835832480136926
41	16.15595029863717	26.939173545677885	35.67848463472505	21.226391520959883
42	17.48972294519715	27.580629824798052	33.78591440910528	21.143732820899515
43	17.33577166916176	27.010996835617984	34.39906594342147	21.25416555179879
44	16.19371944248498	28.620121577724166	34.51188688716358	20.674272092627277
45	16.230604063057662	31.23999106631492	32.84827551130974	19.68112935931768
46	18.28296341832865	29.334904836352138	33.88759035958083	18.49454138573839
47	16.530678584143594	28.780441421340452	35.560887265297275	19.12799272921868
48	16.56061969700655	28.740151386158537	35.071568989498495	19.627659927336413
49	18.426786902863864	26.691949290280636	35.25961863778993	19.62164516906557
50	17.43045988576382	27.911043494220745	34.99404052443385	19.664456095581585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2945.0
1	7086.5
2	11228.0
3	33800.0
4	56372.0
5	40200.5
6	24029.0
7	24005.5
8	23982.0
9	24786.5
10	25591.0
11	24503.5
12	23416.0
13	21592.0
14	19768.0
15	18562.5
16	17357.0
17	17587.5
18	17818.0
19	16001.5
20	14185.0
21	13920.0
22	13655.0
23	16896.5
24	20138.0
25	26287.5
26	32437.0
27	43923.5
28	55410.0
29	64231.5
30	73053.0
31	86259.5
32	99466.0
33	118175.0
34	136884.0
35	144481.5
36	152079.0
37	151513.5
38	150948.0
39	154400.5
40	157853.0
41	162340.0
42	166827.0
43	166439.5
44	166052.0
45	158489.5
46	150927.0
47	149956.5
48	148986.0
49	140775.0
50	132564.0
51	112373.5
52	92183.0
53	80556.5
54	68930.0
55	59357.5
56	49785.0
57	46474.5
58	43164.0
59	38268.0
60	33372.0
61	29934.5
62	26497.0
63	22893.0
64	19289.0
65	16004.5
66	12720.0
67	9996.5
68	7273.0
69	6101.0
70	4929.0
71	4284.5
72	3640.0
73	2722.5
74	1805.0
75	1659.5
76	1514.0
77	1217.0
78	920.0
79	668.0
80	416.0
81	416.5
82	417.0
83	287.5
84	158.0
85	114.0
86	70.0
87	53.0
88	36.0
89	22.0
90	8.0
91	5.0
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2261105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.269371316128684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03584387980952	17.155398120107975
2	9.09586377430952	4.051183356620448
3	3.500416730914192	2.338562398257523
4	1.8737891115812533	1.6691242197568723
5	1.1883444499836944	1.3231841904073802
6	0.8383983027506685	1.1202361868860027
7	0.6211969373811685	0.9683565681288225
8	0.46819012396579673	0.8341039773710918
9	0.40815737937245344	0.8180467412996852
>10	3.661162528708687	18.13210903499555
>50	0.7096589949879051	10.956847672222882
>100	0.5093716837792935	23.658794968201533
>500	0.0741705993482485	11.273744736628634
>1k	0.015235042028288882	4.323521730191191
>5k	0.0	0.0
>10k+	2.0046107931959056E-4	1.376786098924383
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30841	1.3639791164054744	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2360	0.10437374646467103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08323364018919953	0.0
2	0.0	0.0	0.0	0.3154652260730926	0.0
3	0.0	0.0	0.0	0.4537604401387817	0.0
4	0.0	0.0	0.0	0.6868323231340429	0.0
5	0.0	0.0	0.0	1.2098951618788159	0.0
6	0.0	0.0	0.0	1.7058031360772719	0.0
7	0.0	0.0	0.0	2.027327346584966	0.0
8	0.0	0.0	0.0	2.814376156790596	0.0
9	0.0	0.0	0.0	3.123074779808987	0.0
10	0.0	0.0	0.0	3.694697946358086	0.0
11	0.0	0.0	0.0	4.28379044759089	0.0
12	4.422616375621654E-5	0.0	0.0	4.773374080372207	0.0
13	4.422616375621654E-5	0.0	0.0	4.943246775359835	0.0
14	4.422616375621654E-5	0.0	0.0	5.003173227249508	0.0
15	4.422616375621654E-5	0.0	0.0	5.097330729886494	0.0
16	4.422616375621654E-5	0.0	0.0	5.342564807914714	0.0
17	4.422616375621654E-5	0.0	0.0	5.649273253564076	0.0
18	4.422616375621654E-5	0.0	0.0	6.066945144077785	0.0
19	4.422616375621654E-5	0.0	0.0	6.283387989500708	0.0
20	4.422616375621654E-5	0.0	0.0	6.512346839266642	0.0
21	4.422616375621654E-5	0.0	0.0	6.839443546407619	0.0
22	4.422616375621654E-5	0.0	0.0	7.190599286631978	0.0
23	4.422616375621654E-5	0.0	0.0	7.568644534420118	0.0
24	4.422616375621654E-5	0.0	0.0	7.8730089933904	0.0
25	4.422616375621654E-5	0.0	0.0	8.133279967095735	0.0
26	4.422616375621654E-5	0.0	0.0	8.396912129246541	0.0
27	4.422616375621654E-5	0.0	0.0	8.656210127349238	0.0
28	4.422616375621654E-5	0.0	0.0	8.930191211819	0.0
29	4.422616375621654E-5	0.0	0.0	9.241101143025203	0.0
30	4.422616375621654E-5	0.0	0.0	9.632458466103962	0.0
31	4.422616375621654E-5	0.0	0.0	9.971407785131605	0.0
32	4.422616375621654E-5	0.0	0.0	10.264494572344052	0.0
33	4.422616375621654E-5	0.0	0.0	10.573547004672495	0.0
34	4.422616375621654E-5	0.0	0.0	10.899405379228298	0.0
35	4.422616375621654E-5	0.0	0.0	11.288462941791735	0.0
36	4.422616375621654E-5	0.0	0.0	11.618788158886916	0.0
37	4.422616375621654E-5	0.0	0.0	11.94124111883349	0.0
38	4.422616375621654E-5	0.0	0.0	12.292573763712875	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCA	20	7.858098E-4	44.000004	18
TCGACTA	40	8.3182385E-9	44.000004	44
ACGGGTC	185	0.0	44.0	5
TACGCGG	25	4.444212E-5	44.0	2
ACTACGG	35	1.4469151E-7	44.0	2
TTACGAA	165	0.0	44.0	26
TACGTCG	45	4.802132E-10	44.0	42
CGTTTTT	18460	0.0	42.772484	1
CTATGCG	135	0.0	42.37037	1
ATAACGG	125	0.0	42.24	2
CGTAAGA	120	0.0	42.166664	22
ACGGGCC	185	0.0	41.621624	5
TACGATC	270	0.0	41.555553	18
ACGTAGG	165	0.0	41.333332	2
TAGGGAC	2270	0.0	41.09251	5
GCGCGTC	75	0.0	41.066666	38
TGCGTAG	75	0.0	41.066666	1
TCGATAG	215	0.0	40.930233	1
CGACGGT	140	0.0	40.857143	28
GTTTACG	140	0.0	40.857143	1
>>END_MODULE