FastQCFastQC Report
Thu 26 May 2016
SRR1546212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546212_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2324900
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311771.341003914146845No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.10460664974837627No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT207.858144E-444.00000431
GTTGCGA207.858144E-444.00000412
AGCGTAA302.528839E-644.020
ACGGGTC1450.044.05
TAGCCGT351.4469333E-744.044
ATCGTAG254.444251E-544.01
TATCTCG700.044.025
GTGTCGA351.4469333E-744.026
TTGCGAT700.044.032
TTACCGG1000.044.02
TACCGGT302.528839E-644.040
CGTTTTT191250.042.757651
ACGGGTA3600.041.5555575
CGCATGA850.041.41176623
TATACGA850.041.41176630
ATCGACT750.041.06666643
TTCGACA700.040.85714320
ACGCGGC2100.040.85714334
TGCGTAG1350.040.740741
CTATGCG2000.040.6999971
Produced by FastQC (version 0.11.5)