##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546212_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2324900
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784009204696975	31.0	31.0	34.0	30.0	34.0
2	32.191703299066624	33.0	31.0	34.0	30.0	34.0
3	32.26216697492365	33.0	31.0	34.0	30.0	34.0
4	35.959500193556714	37.0	35.0	37.0	35.0	37.0
5	35.85993591122199	37.0	35.0	37.0	35.0	37.0
6	35.942422469783644	37.0	35.0	37.0	35.0	37.0
7	36.185289689879134	37.0	35.0	37.0	35.0	37.0
8	36.08977719471805	37.0	35.0	37.0	35.0	37.0
9	37.83932298163362	39.0	38.0	39.0	35.0	39.0
10	37.38648457998193	39.0	37.0	39.0	34.0	39.0
11	37.25222332143318	39.0	37.0	39.0	34.0	39.0
12	36.997570218073896	39.0	35.0	39.0	33.0	39.0
13	36.85545098713923	39.0	35.0	39.0	33.0	39.0
14	37.9795926706525	40.0	36.0	41.0	33.0	41.0
15	38.152520538517784	40.0	37.0	41.0	33.0	41.0
16	38.19008043356703	40.0	36.0	41.0	33.0	41.0
17	38.104123188094114	40.0	36.0	41.0	33.0	41.0
18	37.952539033936944	39.0	36.0	41.0	33.0	41.0
19	37.799154372231065	39.0	36.0	41.0	34.0	41.0
20	37.60775388188739	39.0	35.0	41.0	33.0	41.0
21	37.519481698137554	39.0	35.0	41.0	33.0	41.0
22	37.4393642737322	39.0	35.0	41.0	33.0	41.0
23	37.39573530044303	39.0	35.0	41.0	33.0	41.0
24	37.34212611295109	39.0	35.0	41.0	33.0	41.0
25	37.315920254634605	39.0	35.0	41.0	33.0	41.0
26	37.29808981031442	39.0	35.0	41.0	33.0	41.0
27	37.229606434685365	39.0	35.0	41.0	33.0	41.0
28	37.157361606950836	39.0	35.0	41.0	33.0	41.0
29	37.09936771474042	39.0	35.0	41.0	33.0	41.0
30	36.92912641403932	39.0	35.0	41.0	32.0	41.0
31	36.712852165684545	39.0	35.0	41.0	31.0	41.0
32	36.46563164007054	39.0	35.0	41.0	31.0	41.0
33	36.15337089767302	39.0	35.0	41.0	30.0	41.0
34	35.83399458041206	39.0	35.0	41.0	27.0	41.0
35	35.62057550862403	39.0	35.0	41.0	25.0	41.0
36	35.49486687599467	39.0	35.0	41.0	24.0	41.0
37	35.391809970321304	39.0	35.0	41.0	24.0	41.0
38	35.31076562432793	39.0	35.0	41.0	23.0	41.0
39	35.25032603552841	39.0	35.0	41.0	23.0	41.0
40	35.15260355284098	39.0	35.0	41.0	23.0	41.0
41	35.06215751215106	39.0	35.0	41.0	23.0	41.0
42	34.97766914706009	39.0	35.0	41.0	22.0	41.0
43	34.897309991827605	38.0	35.0	41.0	22.0	41.0
44	34.79357563766183	38.0	34.0	41.0	21.0	41.0
45	34.74677233429395	38.0	34.0	40.0	21.0	41.0
46	34.70838229601273	38.0	34.0	40.0	22.0	41.0
47	34.66392834100392	38.0	34.0	40.0	21.0	41.0
48	34.60297432147619	38.0	34.0	40.0	21.0	41.0
49	34.526830401307585	38.0	34.0	40.0	20.0	41.0
50	34.38000559163835	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	3.0
12	1.0
13	11.0
14	10.0
15	36.0
16	103.0
17	298.0
18	620.0
19	1316.0
20	2512.0
21	4013.0
22	6767.0
23	10427.0
24	16232.0
25	26539.0
26	41969.0
27	53096.0
28	54084.0
29	49759.0
30	49383.0
31	53795.0
32	63376.0
33	81740.0
34	141836.0
35	227147.0
36	138258.0
37	178751.0
38	323326.0
39	799487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.663039270506257	23.79276528022711	29.52982923996731	18.01436620929932
2	34.13656501354897	23.475590347971956	31.338509183190677	11.049335455288398
3	24.777452793668544	24.560368187879046	39.57365908211106	11.08851993634135
4	23.24986020904125	26.35455288399501	38.18478214116736	12.210804765796377
5	19.84455245386898	30.786872553658224	37.4561916641576	11.912383328315196
6	17.001118327670007	41.369091143705106	33.43533055185169	8.194459976773194
7	83.14847950449482	2.7313002709793968	12.904985160652071	1.2152350638737148
8	83.70312701621575	1.9228353907694955	12.383242289990967	1.990795303023786
9	79.31218547034281	3.5920684760634867	14.441223278420578	2.6545227751731257
10	35.14783431545443	27.859606864811386	22.80338939309218	14.189169426642007
11	25.523463374768806	22.706051873198845	33.9466213600585	17.82386339197385
12	27.480794872897757	20.67301819433094	35.66063056475547	16.18555636801583
13	20.340874876338766	27.36612327411932	36.280313131747604	16.012688717794312
14	14.523076261344576	30.99845154630307	37.33524022538604	17.143231966966322
15	15.082541184567077	25.902103316271667	44.26482859477827	14.750526904382985
16	17.25235493999742	22.760161727386123	42.56638995225601	17.421093380360446
17	16.95113768334122	24.07608929416319	36.60673577358166	22.36603724891393
18	17.732934749881714	24.868983612198374	40.28156049722569	17.116521140694225
19	19.121553615209255	25.997075143016907	36.278033463804896	18.603337777968946
20	20.24254806658351	25.736246720289046	37.36689750096778	16.654307712159664
21	18.538517785711214	25.94468579293733	39.195148178416275	16.32164824293518
22	17.920082584197168	22.92326551679642	37.83810056346509	21.318551335541315
23	15.79001247365478	25.599509656329307	37.882833670265384	20.72764419975053
24	14.989806013161857	24.674136522000946	44.06030366897501	16.275753795862187
25	16.092950234418684	27.008731558346593	38.95505183018624	17.943266377048474
26	15.420663254333519	28.663469396533188	36.489741494257814	19.42612585487548
27	14.866445868639511	29.29674394597617	37.53598004215235	18.300830143231966
28	14.226160264957633	27.405135704761495	40.759645576153815	17.609058454127062
29	15.202417308271324	24.38375844122328	39.435029463632844	20.978794786872555
30	16.110714439330724	28.05750784979999	38.100864553314125	17.730913157555165
31	18.260441309303626	29.4950750569917	34.8688115617876	17.375672071917073
32	18.851864596326724	26.429824938707043	36.43722310637016	18.281087358596068
33	19.064389866230805	27.501655985203666	34.65435072476236	18.779603423803174
34	15.77491505010968	28.139963009161683	35.69504064690954	20.39008129381909
35	16.08735859606865	26.664759774613962	36.32556238978021	20.922319239537185
36	20.41132951954923	28.064346853628113	34.04985160652071	17.47447202030195
37	16.55112047830014	29.04834616542647	36.19656759430513	18.20396576196826
38	16.389048991354464	28.108907910017635	36.17850230117424	19.323540797453653
39	16.92025463460794	28.544023398855867	35.052733450901115	19.48298851563508
40	18.202933459503633	26.33085293991139	35.59563852208697	19.870575078498
41	16.1456836853198	26.921932126112953	35.63452191492107	21.297862273646178
42	17.5740031829326	27.52187190846918	33.75835519807303	21.145769710525183
43	17.26663512409136	26.942105036775775	34.40173770914878	21.389522129984083
44	16.21902017291066	28.570132048690265	34.42750225816164	20.78334552023743
45	16.249258032603553	31.124306421781583	32.7870015914663	19.839433954148568
46	18.279409867091058	29.273216052303326	33.86631683083144	18.581057249774183
47	16.500150544109424	28.760978966837282	35.46315970579379	19.275710783259495
48	16.58712202675384	28.69508365951224	34.98580584111144	19.73198847262248
49	18.44079315239365	26.609660630564758	35.239666222203105	19.709879994838488
50	17.36496193384662	27.85711213385522	34.95918104004473	19.81874489225343
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3123.0
1	7428.5
2	11734.0
3	34396.5
4	57059.0
5	40617.5
6	24176.0
7	23972.5
8	23769.0
9	24927.0
10	26085.0
11	24947.0
12	23809.0
13	22013.0
14	20217.0
15	18957.5
16	17698.0
17	17858.0
18	18018.0
19	16291.5
20	14565.0
21	14314.0
22	14063.0
23	17412.0
24	20761.0
25	27106.5
26	33452.0
27	45170.0
28	56888.0
29	65666.5
30	74445.0
31	87910.5
32	101376.0
33	120287.5
34	139199.0
35	147403.5
36	155608.0
37	155005.0
38	154402.0
39	157862.0
40	161322.0
41	166091.5
42	170861.0
43	170940.5
44	171020.0
45	163542.0
46	156064.0
47	155664.0
48	155264.0
49	146861.5
50	138459.0
51	117848.0
52	97237.0
53	84780.0
54	72323.0
55	62238.0
56	52153.0
57	48390.5
58	44628.0
59	39268.5
60	33909.0
61	30512.5
62	27116.0
63	23371.0
64	19626.0
65	16295.5
66	12965.0
67	10182.0
68	7399.0
69	6153.5
70	4908.0
71	4340.5
72	3773.0
73	2796.0
74	1819.0
75	1664.0
76	1509.0
77	1223.0
78	937.0
79	694.5
80	452.0
81	450.5
82	449.0
83	297.5
84	146.0
85	106.5
86	67.0
87	48.0
88	29.0
89	18.0
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2324900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.231879206636286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9331443814768	17.103683728757012
2	8.992088411429041	3.9982204675656883
3	3.637457973642258	2.4260257886769208
4	1.9263395833955552	1.7130459571604824
5	1.1996187523912758	1.3334889598589286
6	0.8253329961033223	1.1009222084772168
7	0.619886486234834	0.9646869038659326
8	0.4986342519044909	0.8868461165305673
9	0.3817076421817779	0.7637470373911709
>10	3.671828889362424	18.009580325263318
>50	0.716366100648349	11.019942520671384
>100	0.507867402330294	23.564924411865327
>500	0.07232905815435525	10.935125907622634
>1k	0.017202586804279087	4.824814927617389
>5k	0.0	0.0
>10k+	1.9548394095771692E-4	1.3549447386759566
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31177	1.341003914146845	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2432	0.10460664974837627	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08310034840208182	0.0
2	0.0	0.0	0.0	0.31412103746397696	0.0
3	0.0	0.0	0.0	0.4496537485483247	0.0
4	0.0	0.0	0.0	0.6868682523979526	0.0
5	0.0	0.0	0.0	1.213342509355241	0.0
6	0.0	0.0	0.0	1.7247193427674308	0.0
7	0.0	0.0	0.0	2.0582390640457655	0.0
8	0.0	0.0	0.0	2.862703772205256	0.0
9	0.0	0.0	0.0	3.1732117510430555	0.0
10	0.0	0.0	0.0	3.763516710396146	0.0
11	0.0	0.0	0.0	4.381822874102112	0.0
12	0.0	0.0	0.0	4.894576110800465	0.0
13	0.0	0.0	0.0	5.073379500193557	0.0
14	0.0	0.0	0.0	5.134285345606263	0.0
15	0.0	0.0	0.0	5.233859520839606	0.0
16	0.0	0.0	0.0	5.477697965503893	0.0
17	0.0	0.0	0.0	5.797238590907136	0.0
18	0.0	0.0	0.0	6.237945718095402	0.0
19	0.0	0.0	0.0	6.466600714009204	0.0
20	0.0	0.0	0.0	6.7071702008688545	0.0
21	0.0	0.0	0.0	7.051228009806874	0.0
22	0.0	0.0	0.0	7.4240182373435415	0.0
23	0.0	0.0	0.0	7.813497354724935	0.0
24	0.0	0.0	0.0	8.136263925330121	0.0
25	0.0	0.0	0.0	8.412447847219235	0.0
26	0.0	0.0	0.0	8.690782399242979	0.0
27	0.0	0.0	0.0	8.973203148522517	0.0
28	0.0	0.0	0.0	9.2542044819132	0.0
29	0.0	0.0	0.0	9.574691384575681	0.0
30	0.0	0.0	0.0	9.977805497010625	0.0
31	0.0	0.0	0.0	10.32861628457138	0.0
32	4.301260269258893E-5	0.0	0.0	10.631898146156823	0.0
33	4.301260269258893E-5	0.0	0.0	10.960084304701278	0.0
34	4.301260269258893E-5	0.0	0.0	11.297819261043486	0.0
35	4.301260269258893E-5	0.0	0.0	11.701879650737666	0.0
36	4.301260269258893E-5	0.0	0.0	12.038625317217944	0.0
37	4.301260269258893E-5	0.0	0.0	12.375715084519765	0.0
38	4.301260269258893E-5	0.0	0.0	12.740461955352918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	20	7.858144E-4	44.000004	31
GTTGCGA	20	7.858144E-4	44.000004	12
AGCGTAA	30	2.528839E-6	44.0	20
ACGGGTC	145	0.0	44.0	5
TAGCCGT	35	1.4469333E-7	44.0	44
ATCGTAG	25	4.444251E-5	44.0	1
TATCTCG	70	0.0	44.0	25
GTGTCGA	35	1.4469333E-7	44.0	26
TTGCGAT	70	0.0	44.0	32
TTACCGG	100	0.0	44.0	2
TACCGGT	30	2.528839E-6	44.0	40
CGTTTTT	19125	0.0	42.75765	1
ACGGGTA	360	0.0	41.555557	5
CGCATGA	85	0.0	41.411766	23
TATACGA	85	0.0	41.411766	30
ATCGACT	75	0.0	41.066666	43
TTCGACA	70	0.0	40.857143	20
ACGCGGC	210	0.0	40.857143	34
TGCGTAG	135	0.0	40.74074	1
CTATGCG	200	0.0	40.699997	1
>>END_MODULE