FastQCFastQC Report
Thu 26 May 2016
SRR1546211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546211_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences727231
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152242.09342010997881No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24690.3395069792129324No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16630.22867562026371263No Hit
TGTTTTGGGGCTACATGTTTTTATCTTTGAGACAATATTTTTAAGGTTTT13580.18673571396158856No Hit
TATACAGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12140.16693457787140537No Hit
TTAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA11470.1577215492738896No Hit
TTAATAGGGATCACTAAAATAAAGAATATGTTTTTATTCCCAGAGGCATC11380.15648397826825314No Hit
TTTTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11130.15304628103037413No Hit
TTCTTAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC11020.15153369424570734No Hit
ATAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10350.14232066564819157No Hit
TTGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10290.1414956183111006No Hit
TATGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT10280.14135811042158544No Hit
TAGTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10080.13860795263128223No Hit
ATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA9990.13737038162564577No Hit
TGAGAAGGGAAGATTTAGAGGGATAGAATTGAACGGCATTAGGAGAATTG9800.13475773172485772No Hit
TTTGTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA9780.1344827159458274No Hit
GGTAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT9570.131595050266009No Hit
GGAAATGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA9410.12939492403376646No Hit
TTTTATGGGCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACG9410.12939492403376646No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC9310.12801984513861484No Hit
TGTGTAGGGACTTGTGTTGTGGTTTCTCCAGGACAATTTTTTTATTATTA9300.12788233724909967No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC9270.12746981358055418No Hit
TGTTAGGGGTAATTTTAAATAATCTAAGTATAGGTAATATAACAAAAAAG9100.12513217945879646No Hit
TAAAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT8910.12251952955800839No Hit
TTGGCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA8860.1218319901104326No Hit
GGATTTGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA8830.12141946644188711No Hit
GGTTTAGGGTAGGGATAGTCACCTTTTAAATCCTGTTGAAAAGCCATGTT8830.12141946644188711No Hit
AATTGTGGGATTTCCATTTTTGTCTCATTAAATTATAAACTTCATTTAAA8820.12128195855237194No Hit
AGATAAGGGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTT8720.11990687965722033No Hit
TTACATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA8540.11743173764594743No Hit
GGATTTGGGATATCTTTTCTTTAAACCGTTTTCTGTTTCTTGGTCTATTA8410.11564413508225034No Hit
TATATTGGGGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGG8410.11564413508225034No Hit
TGGCTAGGGAACTTGAATCTTTTCTTGCATTGTATTTTTCTAAAGTTTGT8370.1150941035241897No Hit
AGTGCTGGGTCATGAATTAAAAGTTTTATCTGGTATAAATAAAAGTAAAT8050.11069385105970456No Hit
TGACCCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC8050.11069385105970456No Hit
TAATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA8030.11041883528067423No Hit
TTGCTAGGGAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT7950.10931877216455294No Hit
TTTGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC7940.10918126427503778No Hit
GTACGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT7830.10766867749037101No Hit
ATTATAGGGATCATATATGGGCTTTGTTTCTTTTCATTGATTTTTCTTAG7790.10711864593231037No Hit
TGTACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT7700.10588107492667392No Hit
TTTATAGGGACTGTTTTGCTGAGGAAAATGACTTGTTAGAGACTTCAGTT7680.1056060591476436No Hit
TAAAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA7590.10436848814200715No Hit
AAATATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC7580.104230980252492No Hit
TTATTAAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG7540.10368094869443135No Hit
ATAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC7530.10354344080491618No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7450.10244337768879488No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT7430.10216836190976458No Hit
CTAAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT7360.10120580668315844No Hit
TAGTTAGGGCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATA7280.10010574356703716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG302.526429E-644.0000041
ACGTTAG302.526429E-644.0000041
GGGTCAA302.526429E-644.0000047
ATGTGTC302.526429E-644.00000432
CGCATCT302.526429E-644.00000439
AGGACTA302.526429E-644.0000046
GCAGTAC600.044.00000438
CGTCTGG302.526429E-644.0000042
TCGATTG302.526429E-644.0000043
CACGAAT600.044.00000424
TAGCCGT302.526429E-644.00000444
ATGTATC600.044.00000432
GTAGCCG302.526429E-644.00000443
TCTGTAG302.526429E-644.0000041
GGTATCA302.526429E-644.0000048
AGTCTAT302.526429E-644.00000430
CGAATGT600.044.00000426
GTATCAC302.526429E-644.0000049
CATAAGT302.526429E-644.00000423
TACAGTC302.526429E-644.00000427
Produced by FastQC (version 0.11.5)