FastQCFastQC Report
Thu 26 May 2016
SRR1546210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546210_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences726030
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150812.077186893103591No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25310.34860818423481127No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15730.21665771386857294No Hit
TGTTTTGGGGCTACATGTTTTTATCTTTGAGACAATATTTTTAAGGTTTT13380.18428990537581091No Hit
TTTTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11240.15481453934410425No Hit
TTAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA11050.1521975675936256No Hit
TTAATAGGGATCACTAAAATAAAGAATATGTTTTTATTCCCAGAGGCATC10970.15109568475131882No Hit
TATACAGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10940.15068247868545376No Hit
TTCTTAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC10620.14627494731622662No Hit
GGTAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10210.1406277977494043No Hit
ATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA10170.1400768563282509No Hit
TTGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10050.1384240320647907No Hit
TAGTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT9960.13718441386719557No Hit
TATGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT9940.1369089431566189No Hit
TTTTATGGGCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACG9840.13553158960373538No Hit
ATAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT9790.13484291282729363No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC9700.1336032946296985No Hit
TGTGTAGGGACTTGTGTTGTGGTTTCTCCAGGACAATTTTTTTATTATTA9640.1327768824979684No Hit
TGAGAAGGGAAGATTTAGAGGGATAGAATTGAACGGCATTAGGAGAATTG9570.13181273501094998No Hit
GGAAATGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA9530.13126179358979656No Hit
TTTGTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA9530.13126179358979656No Hit
GGATTTGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA9340.12864482183931794No Hit
TTGGCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA9240.12726746828643443No Hit
TAAAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT9080.12506370260182087No Hit
TTACATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA8780.1209316419431704No Hit
AATTGTGGGATTTCCATTTTTGTCTCATTAAATTATAAACTTCATTTAAA8710.11996749445615194No Hit
GGTTTAGGGTAGGGATAGTCACCTTTTAAATCCTGTTGAAAAGCCATGTT8640.11900334696913352No Hit
TGGCTAGGGAACTTGAATCTTTTCTTGCATTGTATTTTTCTAAAGTTTGT8610.11859014090326846No Hit
TGTTAGGGGTAATTTTAAATAATCTAAGTATAGGTAATATAACAAAAAAG8610.11859014090326846No Hit
AGATAAGGGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTT8550.11776372877153837No Hit
TATATTGGGGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGG8540.11762599341625002No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC8450.11638637521865487No Hit
TTGCTAGGGAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT8110.11170337313885102No Hit
GGATTTGGGATATCTTTTCTTTAAACCGTTTTCTGTTTCTTGGTCTATTA8060.11101469636240927No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT7930.10922413674366073No Hit
TAATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA7820.10770904783548889No Hit
TGACCCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC7820.10770904783548889No Hit
TTTGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC7760.1068826357037588No Hit
AGTGCTGGGTCATGAATTAAAAGTTTTATCTGGTATAAATAAAAGTAAAT7730.10646942963789376No Hit
ATTATAGGGATCATATATGGGCTTTGTTTCTTTTCATTGATTTTTCTTAG7620.10495434072972191No Hit
ATAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC7590.10454113466385688No Hit
TAGTTAGGGCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATA7400.10192416291337823No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7400.10192416291337823No Hit
AAATATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC7330.10096001542635981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG408.305506E-944.0000041
GTGTTAG207.854562E-444.0000041
ACAACAG207.854562E-444.00000412
AAGTAGC207.854562E-444.00000437
TAATACG207.854562E-444.0000041
GGTACTC207.854562E-444.0000048
GCCTTAC207.854562E-444.00000423
GATAAGT207.854562E-444.00000434
CTCCGCT207.854562E-444.00000413
CATGCGG207.854562E-444.0000042
TTAGCCA207.854562E-444.00000440
CAGCATT207.854562E-444.00000437
ATGTGCG207.854562E-444.0000041
TGTGGAC207.854562E-444.00000426
GCTATGT650.044.0000049
GAATCAC800.044.0000049
ACTTCGA207.854562E-444.00000433
GTTAGTC207.854562E-444.00000436
GGTCATA207.854562E-444.0000048
AGGCGTA207.854562E-444.00000413
Produced by FastQC (version 0.11.5)