##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546210_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	726030
Sequences flagged as poor quality	0
Sequence length	50
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53782763797639	33.0	31.0	34.0	31.0	34.0
2	32.94090877787419	34.0	31.0	34.0	31.0	34.0
3	32.82628128314257	34.0	31.0	34.0	31.0	34.0
4	36.436181700480695	37.0	37.0	37.0	35.0	37.0
5	36.41733950387725	37.0	37.0	37.0	35.0	37.0
6	36.50382353346281	37.0	37.0	37.0	35.0	37.0
7	36.58311777750231	37.0	37.0	37.0	35.0	37.0
8	36.53765546878228	37.0	37.0	37.0	35.0	37.0
9	38.39340660854235	39.0	39.0	39.0	37.0	39.0
10	38.267052325661474	39.0	39.0	39.0	37.0	39.0
11	38.1006459788163	39.0	38.0	39.0	35.0	39.0
12	37.29054722256656	39.0	35.0	39.0	35.0	39.0
13	37.082862967095025	39.0	35.0	39.0	35.0	39.0
14	38.32960896932634	40.0	35.0	41.0	35.0	41.0
15	38.49057339228406	40.0	35.0	41.0	35.0	41.0
16	38.57147087585913	41.0	35.0	41.0	35.0	41.0
17	38.51734225858436	40.0	35.0	41.0	35.0	41.0
18	38.186942688318666	39.0	36.0	41.0	35.0	41.0
19	37.759432805806924	37.0	36.0	41.0	35.0	41.0
20	37.2438260126992	36.0	35.0	41.0	35.0	41.0
21	37.1871286310483	36.0	35.0	41.0	35.0	41.0
22	37.16010908640139	35.0	35.0	41.0	35.0	41.0
23	37.095768769885545	35.0	35.0	41.0	35.0	41.0
24	37.03138851011666	35.0	35.0	41.0	35.0	41.0
25	36.99363111717147	35.0	35.0	41.0	35.0	41.0
26	36.96604134815366	35.0	35.0	41.0	35.0	41.0
27	36.87452446868587	35.0	35.0	41.0	34.0	41.0
28	36.79995179262565	36.0	35.0	41.0	34.0	41.0
29	36.648973182926326	36.0	35.0	41.0	34.0	41.0
30	36.334112915444265	36.0	35.0	41.0	34.0	41.0
31	35.80140352327039	35.0	35.0	41.0	33.0	41.0
32	35.0719832513808	35.0	35.0	41.0	29.0	41.0
33	34.27690315827169	35.0	35.0	41.0	18.0	41.0
34	33.56912937481922	35.0	35.0	41.0	14.0	41.0
35	33.08783108136027	35.0	34.0	41.0	10.0	41.0
36	32.86571904742228	35.0	34.0	40.0	10.0	41.0
37	32.716295469884166	35.0	34.0	40.0	10.0	41.0
38	32.67859317108108	35.0	33.0	40.0	10.0	41.0
39	32.60311281902952	35.0	33.0	40.0	10.0	41.0
40	32.427406580995275	35.0	33.0	40.0	8.0	41.0
41	32.27624065121276	35.0	33.0	40.0	8.0	41.0
42	32.137194055342064	35.0	33.0	40.0	7.0	41.0
43	31.988727738523203	35.0	33.0	40.0	7.0	41.0
44	31.787572138892333	35.0	33.0	40.0	7.0	41.0
45	31.707973499717642	35.0	32.0	40.0	7.0	41.0
46	31.65570568709282	35.0	32.0	40.0	7.0	41.0
47	31.610999545473327	35.0	32.0	40.0	7.0	41.0
48	31.582372629230196	35.0	32.0	40.0	7.0	41.0
49	31.521985317411126	35.0	32.0	40.0	7.0	41.0
50	31.308056140930816	35.0	31.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	5.0
14	10.0
15	17.0
16	28.0
17	54.0
18	103.0
19	261.0
20	492.0
21	921.0
22	1864.0
23	3471.0
24	7218.0
25	14456.0
26	25963.0
27	32137.0
28	28765.0
29	20772.0
30	15710.0
31	12477.0
32	12926.0
33	16933.0
34	46172.0
35	142868.0
36	28045.0
37	31382.0
38	65867.0
39	217106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.81296916105395	21.893172458438357	27.21939864743881	28.074459733068878
2	42.38034241009325	21.52913791441125	27.393771607233862	8.696748068261643
3	22.1255320028098	22.24067875983086	46.842554715369886	8.79123452198945
4	19.726733055107914	24.51937247772131	45.405561753646545	10.348332713524234
5	16.88428852802226	26.905775243447238	45.859262013966365	10.350674214564135
6	14.454223654669917	34.963568998526235	42.488740134705175	8.093467212098673
7	69.85537787694723	4.3003732628128315	24.641543737862072	1.202705122377863
8	71.18121840695288	3.316667355343443	23.89419170006749	1.6079225376361856
9	68.42706224260705	4.240182912551823	25.380356183628777	1.9523986612123467
10	43.08692478272248	18.737242262716418	29.548503505364792	8.627329449196314
11	38.87525308871535	16.043689654697467	35.52263680564164	9.558420450945553
12	33.91609162155834	17.361541534096386	39.36448907069956	9.357877773645718
13	14.15437378620718	37.58687657534813	38.48670165144691	9.772047986997782
14	9.101001336032946	37.45699213531121	43.46073853697506	9.981267991680784
15	8.002837348318941	19.45195102130766	63.181135765739704	9.364075864633694
16	8.769334600498603	16.908805421263583	59.40167761662741	14.9201823616104
17	9.0081677065686	17.27283996529069	43.095739845460926	30.62325248267978
18	13.351376664876108	21.149126069170695	50.966488988058344	14.53300827789485
19	18.949767915926337	20.94610415547567	41.592909383909756	18.511218544688234
20	19.453879316281697	18.22307618142501	45.964078619340796	16.358965882952496
21	14.027932730052479	25.188215363001532	45.32236959905238	15.461482307893611
22	16.899852623169842	20.240761401044033	39.211878297040066	23.647507678746056
23	11.081773480434693	23.692960345991214	41.004090740052064	24.22117543352203
24	11.567015137115547	18.993430023552747	56.44229577290195	12.997259066429761
25	10.677795683373965	21.987658912166165	52.563117226560884	14.771428177898985
26	10.798451854606558	26.9476467914549	44.113328650331255	18.140572703607287
27	9.980992520970208	33.55742875638747	41.242510640056196	15.219068082586118
28	9.271930911945788	28.588074872939135	47.10177265402256	15.038221561092517
29	10.076305386829745	22.778122116166	46.3765960084294	20.768976488574854
30	11.935181741801303	31.5108191121579	41.59401126675206	14.959987879288736
31	18.820985358731733	29.363800393923118	39.113948459430055	12.7012657879151
32	19.635827720617606	23.21019792570555	42.576064349957996	14.577910003718856
33	21.76204840020385	27.948569618335327	34.83244494029172	15.456937041169097
34	14.999242455545915	27.348043469278128	34.835475118108064	22.81723895706789
35	15.248681183973115	25.475669049488314	38.53793920361417	20.7377105629244
36	25.710783300965524	25.37925430078647	34.876933460049855	14.033028938198147
37	14.799663925733098	30.280153712656503	39.74243488561079	15.177747475999615
38	15.933363635111498	29.448507637425454	34.15134360839084	20.466785119072213
39	15.949340936324946	29.754280126165582	36.24464553806316	18.051733399446306
40	17.876120821453657	24.36965414652287	34.14542098811344	23.60880404391003
41	12.222773163643376	26.39271104499814	37.43633183201796	23.948183959340525
42	16.257317190749692	27.137583846397533	34.109472060383176	22.495626902469596
43	18.455573461151744	23.486908254479843	35.20818698951834	22.849331294850074
44	13.77160723386086	29.131578584906958	34.93891436992962	22.157899811302563
45	16.08721402696858	35.96614464967012	29.034888365494538	18.911752957866753
46	22.14398854041844	29.783755492197294	31.86479897524896	16.20745699213531
47	17.728881726650414	30.30356872305552	32.14398854041844	19.823561009875625
48	17.42572620966076	29.83154966048235	34.288528022258035	18.45419610759886
49	20.690880542126358	24.72432268639037	35.65637783562663	18.928418935856644
50	18.7409611173092	29.83664586862802	31.43823257992094	19.98416043414184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3042.0
1	3458.5
2	3875.0
3	15273.5
4	26672.0
5	19635.5
6	12599.0
7	14474.5
8	16350.0
9	18101.5
10	19853.0
11	19899.0
12	19945.0
13	18574.5
14	17204.0
15	15388.5
16	13573.0
17	12119.5
18	10666.0
19	9637.0
20	8608.0
21	9088.0
22	9568.0
23	8249.5
24	6931.0
25	7256.5
26	7582.0
27	10511.0
28	13440.0
29	12920.5
30	12401.0
31	17971.0
32	23541.0
33	25932.0
34	28323.0
35	30626.0
36	32929.0
37	34034.5
38	35140.0
39	39678.5
40	44217.0
41	52358.0
42	60499.0
43	62925.0
44	65351.0
45	60550.5
46	55750.0
47	49955.0
48	44160.0
49	39747.5
50	35335.0
51	29731.5
52	24128.0
53	20482.0
54	16836.0
55	14799.5
56	12763.0
57	11812.0
58	10861.0
59	9796.5
60	8732.0
61	8435.0
62	8138.0
63	6893.5
64	5649.0
65	4842.0
66	4035.0
67	3254.5
68	2474.0
69	2158.0
70	1842.0
71	1600.5
72	1359.0
73	977.5
74	596.0
75	555.5
76	515.0
77	404.5
78	294.0
79	234.5
80	175.0
81	102.5
82	30.0
83	33.0
84	36.0
85	22.5
86	9.0
87	5.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	726030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.789186658368617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49394083114836	20.500840783037955
2	8.628115034140887	4.450241382506716
3	3.0807492695506338	2.383500538802222
4	1.6077640156828346	1.6585170521221164
5	0.9409937619393929	1.2133731885507741
6	0.658260835000144	1.018560692622738
7	0.4954745664386968	0.8944520254853271
8	0.37775007780163944	0.7793493813311797
9	0.30374440181469314	0.7049988967350276
>10	3.337797123758918	20.066773220777335
>50	0.599118734365309	10.647347490294855
>100	0.4135367450274415	21.722115976575473
>500	0.056318233758600976	9.941895360997883
>1k	0.005900005441377245	1.9319705666452045
>5k	0.0	0.0
>10k+	5.363641310342951E-4	2.0860634435151666
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15081	2.077186893103591	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2531	0.34860818423481127	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1573	0.21665771386857294	No Hit
TGTTTTGGGGCTACATGTTTTTATCTTTGAGACAATATTTTTAAGGTTTT	1338	0.18428990537581091	No Hit
TTTTGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1124	0.15481453934410425	No Hit
TTAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1105	0.1521975675936256	No Hit
TTAATAGGGATCACTAAAATAAAGAATATGTTTTTATTCCCAGAGGCATC	1097	0.15109568475131882	No Hit
TATACAGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1094	0.15068247868545376	No Hit
TTCTTAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	1062	0.14627494731622662	No Hit
GGTAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1021	0.1406277977494043	No Hit
ATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA	1017	0.1400768563282509	No Hit
TTGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1005	0.1384240320647907	No Hit
TAGTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	996	0.13718441386719557	No Hit
TATGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	994	0.1369089431566189	No Hit
TTTTATGGGCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACG	984	0.13553158960373538	No Hit
ATAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	979	0.13484291282729363	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	970	0.1336032946296985	No Hit
TGTGTAGGGACTTGTGTTGTGGTTTCTCCAGGACAATTTTTTTATTATTA	964	0.1327768824979684	No Hit
TGAGAAGGGAAGATTTAGAGGGATAGAATTGAACGGCATTAGGAGAATTG	957	0.13181273501094998	No Hit
GGAAATGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA	953	0.13126179358979656	No Hit
TTTGTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	953	0.13126179358979656	No Hit
GGATTTGGGCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAA	934	0.12864482183931794	No Hit
TTGGCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA	924	0.12726746828643443	No Hit
TAAAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	908	0.12506370260182087	No Hit
TTACATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	878	0.1209316419431704	No Hit
AATTGTGGGATTTCCATTTTTGTCTCATTAAATTATAAACTTCATTTAAA	871	0.11996749445615194	No Hit
GGTTTAGGGTAGGGATAGTCACCTTTTAAATCCTGTTGAAAAGCCATGTT	864	0.11900334696913352	No Hit
TGGCTAGGGAACTTGAATCTTTTCTTGCATTGTATTTTTCTAAAGTTTGT	861	0.11859014090326846	No Hit
TGTTAGGGGTAATTTTAAATAATCTAAGTATAGGTAATATAACAAAAAAG	861	0.11859014090326846	No Hit
AGATAAGGGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTT	855	0.11776372877153837	No Hit
TATATTGGGGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGG	854	0.11762599341625002	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC	845	0.11638637521865487	No Hit
TTGCTAGGGAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT	811	0.11170337313885102	No Hit
GGATTTGGGATATCTTTTCTTTAAACCGTTTTCTGTTTCTTGGTCTATTA	806	0.11101469636240927	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	793	0.10922413674366073	No Hit
TAATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	782	0.10770904783548889	No Hit
TGACCCAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	782	0.10770904783548889	No Hit
TTTGTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	776	0.1068826357037588	No Hit
AGTGCTGGGTCATGAATTAAAAGTTTTATCTGGTATAAATAAAAGTAAAT	773	0.10646942963789376	No Hit
ATTATAGGGATCATATATGGGCTTTGTTTCTTTTCATTGATTTTTCTTAG	762	0.10495434072972191	No Hit
ATAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	759	0.10454113466385688	No Hit
TAGTTAGGGCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATA	740	0.10192416291337823	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	740	0.10192416291337823	No Hit
AAATATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	733	0.10096001542635981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04283569549467653	0.0
2	0.0	0.0	0.0	0.22354448163299037	0.0
3	0.0	0.0	0.0	0.3439251821550074	0.0
4	0.0	0.0	0.0	0.5658168395245375	0.0
5	0.0	0.0	0.0	0.9658003112819029	0.0
6	0.0	0.0	0.0	1.3493932757599547	0.0
7	0.0	0.0	0.0	1.5627453411016075	0.0
8	0.0	0.0	0.0	2.029117254107957	0.0
9	0.0	0.0	0.0	2.2570692671101744	0.0
10	0.0	0.0	0.0	2.6715149511728167	0.0
11	0.0	0.0	0.0	3.2132281035218933	0.0
12	0.0	0.0	0.0	3.618996460201369	0.0
13	0.0	0.0	0.0	3.7813864440863325	0.0
14	0.0	0.0	0.0	3.838408881175709	0.0
15	0.0	0.0	0.0	3.913750120518436	0.0
16	0.0	0.0	0.0	4.109885266449044	0.0
17	0.0	0.0	0.0	4.342658016886355	0.0
18	0.0	0.0	0.0	4.745396195749487	0.0
19	0.0	0.0	0.0	4.917703125215212	0.0
20	0.0	0.0	0.0	5.16053055658857	0.0
21	0.0	0.0	0.0	5.411071167858077	0.0
22	0.0	0.0	0.0	5.633238295938185	0.0
23	0.0	0.0	0.0	5.92812969161054	0.0
24	0.0	0.0	0.0	6.123713896119995	0.0
25	0.0	0.0	0.0	6.262000192829498	0.0
26	0.0	0.0	0.0	6.444086332520695	0.0
27	0.0	0.0	0.0	6.612674407393634	0.0
28	0.0	0.0	0.0	6.813768026114623	0.0
29	0.0	0.0	0.0	6.984835337382753	0.0
30	0.0	0.0	0.0	7.255485310524358	0.0
31	0.0	0.0	0.0	7.5229673704943325	0.0
32	0.0	0.0	0.0	7.740589231849923	0.0
33	0.0	0.0	0.0	7.936311171714667	0.0
34	0.0	0.0	0.0	8.1463575885294	0.0
35	0.0	0.0	0.0	8.485737503959891	0.0
36	0.0	0.0	0.0	8.70611407242125	0.0
37	0.0	0.0	0.0	8.94632453204413	0.0
38	0.0	0.0	0.0	9.14480117901464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	40	8.305506E-9	44.000004	1
GTGTTAG	20	7.854562E-4	44.000004	1
ACAACAG	20	7.854562E-4	44.000004	12
AAGTAGC	20	7.854562E-4	44.000004	37
TAATACG	20	7.854562E-4	44.000004	1
GGTACTC	20	7.854562E-4	44.000004	8
GCCTTAC	20	7.854562E-4	44.000004	23
GATAAGT	20	7.854562E-4	44.000004	34
CTCCGCT	20	7.854562E-4	44.000004	13
CATGCGG	20	7.854562E-4	44.000004	2
TTAGCCA	20	7.854562E-4	44.000004	40
CAGCATT	20	7.854562E-4	44.000004	37
ATGTGCG	20	7.854562E-4	44.000004	1
TGTGGAC	20	7.854562E-4	44.000004	26
GCTATGT	65	0.0	44.000004	9
GAATCAC	80	0.0	44.000004	9
ACTTCGA	20	7.854562E-4	44.000004	33
GTTAGTC	20	7.854562E-4	44.000004	36
GGTCATA	20	7.854562E-4	44.000004	8
AGGCGTA	20	7.854562E-4	44.000004	13
>>END_MODULE