FastQCFastQC Report
Thu 26 May 2016
SRR1546209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546209_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1673779
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293191.7516649450136486No Hit
GAGTATGGGGTTTTTGTCTTTGAGTTTGTTTATAAAGTGGATTACATTGC49960.29848623982019135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39510.236052668840988No Hit
TCAAATGGGTAATTATAGCCTTTATTCTAGTCACAATTCTATCTCTAGGC29480.17612838970975261No Hit
AGGAAAGGAGAGGAAAGGAGAGGAAAGAGGGAAGAGGGAAGAGGGAAGAG24870.14858592442610405No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22080.13191705715031674No Hit
TGTATAGGGACATTTCTATTATTTGACCTAGAAATTGCTCTTCTACTTCC21000.12546459239839908No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC20550.12277606541843337No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA19640.11733926641450275No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC18090.1080787845946209No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC17760.10610719814264608No Hit
CCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTG16900.10096912435871162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG650.044.0000041
TGCACAT1450.044.00000412
TCACGAG800.044.01
TGTCACG254.4437133E-544.01
CTAGACG351.4466059E-744.01
AATCCGT351.4466059E-744.020
GTACCGT254.4437133E-544.09
GTACCGG351.4466059E-744.02
GGTCGTT302.5284116E-644.08
GGTCGGA800.044.08
GTCTGCA302.5284116E-644.034
CTCCGAA207.857513E-444.044
CGACGTC351.4466059E-744.018
TGGACCG207.857513E-444.017
CATTGCG750.044.01
GACCGTT254.4437133E-544.040
GACCGGA207.857513E-444.041
CACGCTA408.3164196E-944.026
TCGGGTA502.7284841E-1144.05
ATAGCCG254.4437133E-544.02
Produced by FastQC (version 0.11.5)