##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546209_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1673779
Sequences flagged as poor quality	0
Sequence length	50
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899404282166284	31.0	31.0	34.0	30.0	34.0
2	32.24895819579526	33.0	31.0	34.0	30.0	34.0
3	32.09105801900968	33.0	31.0	34.0	30.0	34.0
4	35.88105657915412	37.0	35.0	37.0	35.0	37.0
5	35.88840581701646	37.0	35.0	37.0	35.0	37.0
6	35.966082738521635	37.0	35.0	37.0	35.0	37.0
7	36.18242850459947	37.0	35.0	37.0	35.0	37.0
8	36.07617074894595	37.0	35.0	37.0	35.0	37.0
9	37.859966578622384	39.0	38.0	39.0	35.0	39.0
10	37.57268611925469	39.0	37.0	39.0	35.0	39.0
11	37.35174058223935	39.0	37.0	39.0	34.0	39.0
12	36.74156564277602	39.0	35.0	39.0	33.0	39.0
13	36.541018856133334	39.0	35.0	39.0	33.0	39.0
14	37.69494300024077	40.0	35.0	41.0	33.0	41.0
15	37.89596296763193	40.0	35.0	41.0	33.0	41.0
16	38.001191913627785	40.0	35.0	41.0	34.0	41.0
17	37.91889192061795	40.0	35.0	41.0	34.0	41.0
18	37.63716237328823	39.0	36.0	41.0	34.0	41.0
19	37.31315902517596	37.0	36.0	41.0	34.0	41.0
20	36.94938459617428	37.0	35.0	41.0	34.0	41.0
21	36.84175031470702	37.0	35.0	41.0	33.0	41.0
22	36.73035329036868	37.0	35.0	41.0	33.0	41.0
23	36.717382043866	37.0	35.0	41.0	33.0	41.0
24	36.60259687808247	37.0	35.0	41.0	33.0	41.0
25	36.58938306670116	37.0	35.0	40.0	33.0	41.0
26	36.49911786442535	37.0	35.0	41.0	33.0	41.0
27	36.417521668033835	37.0	35.0	41.0	33.0	41.0
28	36.332828288561394	37.0	35.0	41.0	32.0	41.0
29	36.1881550670668	36.0	35.0	41.0	32.0	41.0
30	35.90752960815018	36.0	35.0	40.0	31.0	41.0
31	35.48060466764131	36.0	35.0	40.0	30.0	41.0
32	34.94607591563761	36.0	35.0	41.0	24.0	41.0
33	34.33716637620618	36.0	34.0	41.0	21.0	41.0
34	33.66891566927294	36.0	34.0	40.0	15.0	41.0
35	33.28959856707486	36.0	33.0	40.0	12.0	41.0
36	33.00050962522531	36.0	33.0	40.0	10.0	41.0
37	32.95562018641649	36.0	33.0	40.0	10.0	41.0
38	32.90181260488989	36.0	33.0	40.0	10.0	41.0
39	32.827894244102716	36.0	33.0	40.0	10.0	41.0
40	32.63688874098671	36.0	32.0	40.0	10.0	41.0
41	32.52789107761539	36.0	32.0	40.0	10.0	41.0
42	32.433136035283034	36.0	32.0	40.0	9.0	41.0
43	32.256462173321566	35.0	32.0	40.0	8.0	41.0
44	32.07371881234022	35.0	31.0	40.0	8.0	41.0
45	32.0046893885035	35.0	31.0	40.0	8.0	41.0
46	31.9074776299619	35.0	31.0	40.0	8.0	41.0
47	31.919400948392827	35.0	31.0	40.0	8.0	41.0
48	31.829589211000975	35.0	31.0	40.0	8.0	41.0
49	31.687923555021303	35.0	30.0	40.0	8.0	41.0
50	31.469223236759454	35.0	30.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	4.0
13	6.0
14	17.0
15	42.0
16	104.0
17	355.0
18	857.0
19	1767.0
20	3119.0
21	5524.0
22	9176.0
23	14586.0
24	23086.0
25	38314.0
26	58018.0
27	65186.0
28	55969.0
29	44343.0
30	41078.0
31	41641.0
32	48299.0
33	61186.0
34	113004.0
35	240485.0
36	79327.0
37	99070.0
38	171532.0
39	457676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.13971139559046	23.1034085145052	26.996276091407527	23.760603998496816
2	38.53889910197225	25.374735852224216	26.151242189082307	9.935122856721227
3	23.50393928947609	23.677498642293877	42.93177295210419	9.886789116125845
4	21.001159651304025	27.12861136386584	42.062960522267275	9.80726846256286
5	19.60593363878983	29.634318509193868	40.61276906927378	10.146978782742524
6	15.465004639202668	38.252541106083896	37.87919432613266	8.403259928580773
7	74.48773105648954	4.566194222773736	19.68431913651683	1.2617555842199
8	74.66857930467523	4.436368242163391	19.030529120033172	1.8645233331282085
9	71.34938364025358	6.083300125046377	20.614011766188966	1.9533044685110759
10	39.389847763653385	27.95094214947135	25.663603139960532	6.9956069469147355
11	35.118555078059885	18.717405344433168	36.02249759376836	10.141541983738595
12	34.53884891613529	20.67871564884014	35.75501903178376	9.02741640324081
13	17.39584497117003	38.79233757861701	32.667693883123164	11.1441235670898
14	10.314085670808392	40.7216842844844	35.216417460130636	13.747812584576577
15	9.129819408655504	27.284545928703853	52.17540666957824	11.410227993062405
16	10.579413411209007	21.580328107832635	49.751968449837165	18.088290031121193
17	12.191454188396438	22.653169862926944	37.24374603815677	27.911629910519846
18	14.082145850796312	27.513070722000933	44.05002094063792	14.354762486564834
19	17.49466327394477	27.423632391134074	37.18328405362954	17.898420281291617
20	20.86846590858172	22.531588698388497	41.255327017485584	15.344618375544202
21	15.935078645388668	28.886788518675406	39.57541587031502	15.602716965620909
22	19.461290887267673	25.281892053849404	33.58382438780747	21.672992671075452
23	14.236108829182347	28.92950622513486	34.12756403324453	22.70682091243826
24	11.752925565442032	24.68820555162898	51.851469041014376	11.707399841914613
25	12.312975607890886	30.45157096605944	43.384520895530414	13.85093253051926
26	12.243133651455777	32.34943203373922	37.76968166048206	17.63775265432294
27	11.406583545378451	37.927468321684046	34.463092200344256	16.202855932593252
28	10.593871711856822	32.49712178250534	41.187695627678444	15.721310877959397
29	11.25937175696433	24.143091770179932	41.59491784757725	23.002618625278487
30	12.337530820974575	35.523029025934726	37.140387112038084	14.999053041052612
31	18.585727267458847	35.21074168095071	33.456388208957094	12.747142842633346
32	19.15587422234357	27.974123226543053	34.878380001182954	17.991622549930426
33	19.154739066507588	35.49548656065108	30.248198836286033	15.101575536555304
34	15.378673050623767	30.518545160382583	32.32177007836757	21.781011710626075
35	18.87381786962317	29.16287036699588	33.39437285328589	18.56893891009506
36	24.567879033014513	32.05106528400703	28.981842883678194	14.399212799300265
37	15.689407024463803	35.436099986915835	34.926713741778336	13.947779246842027
38	14.942593974473334	30.664980263224717	37.27481346103637	17.11761230126558
39	15.980903094136083	32.89281320891229	34.787149318996114	16.339134377955514
40	19.509624627863058	28.764669648740966	32.296318689623895	19.42938703377208
41	13.997128653185397	30.04614109748061	34.854481983583256	21.102248265750738
42	16.6455667086276	28.943845035694675	31.08247863069139	23.328109624986336
43	17.046814424126485	28.667344972066207	33.07658896425394	21.20925163955337
44	15.361884693259983	30.265285918869818	31.997055764231717	22.375773623638484
45	14.741312921239903	40.067954013044734	26.087912442443116	19.102820623272248
46	18.63555463415421	36.257235871641356	29.78356162910396	15.32364786510047
47	16.739426172750406	32.429848862962196	32.2963784346679	18.5343465296195
48	17.94920356869097	28.501134259660326	34.88417527045088	18.665486901197827
49	20.082400364683746	28.835348035792062	32.710292099494616	18.371959500029575
50	17.48522355699289	32.071378598966774	30.381191304228334	20.062206539812006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4935.0
1	5368.5
2	5802.0
3	23760.5
4	41719.0
5	32447.5
6	23176.0
7	25765.0
8	28354.0
9	31083.5
10	33813.0
11	33972.5
12	34132.0
13	32490.0
14	30848.0
15	28278.5
16	25709.0
17	23799.5
18	21890.0
19	20253.5
20	18617.0
21	17231.0
22	15845.0
23	15200.0
24	14555.0
25	17655.0
26	20755.0
27	24717.5
28	28680.0
29	36698.5
30	44717.0
31	52448.5
32	60180.0
33	80372.0
34	100564.0
35	108100.5
36	115637.0
37	104421.5
38	93206.0
39	109658.5
40	126111.0
41	134923.5
42	143736.0
43	150762.0
44	157788.0
45	144335.0
46	130882.0
47	120989.0
48	111096.0
49	101087.0
50	91078.0
51	74233.5
52	57389.0
53	46429.5
54	35470.0
55	27970.0
56	20470.0
57	16009.0
58	11548.0
59	9648.5
60	7749.0
61	6866.0
62	5983.0
63	5077.5
64	4172.0
65	3321.0
66	2470.0
67	1952.0
68	1434.0
69	1296.5
70	1159.0
71	1030.5
72	902.0
73	712.0
74	522.0
75	419.0
76	316.0
77	241.0
78	166.0
79	132.5
80	99.0
81	66.0
82	33.0
83	38.5
84	44.0
85	29.0
86	14.0
87	10.0
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1673779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.099933543026857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30614091979919	19.905788666408828
2	9.150353826769413	4.593465458941876
3	3.5781228686849484	2.694319386383305
4	1.8987434838318047	1.906333410377344
5	1.1015913003450755	1.382493421511897
6	0.7332057659430617	1.1042049599120973
7	0.5072955184546533	0.8913158659921006
8	0.3711200510580367	0.7452070894433165
9	0.2702587335988573	0.6105128627478527
>10	2.04651014034019	10.700881952098056
>50	0.47553607324245517	8.610224705369712
>100	0.46214209954696134	23.758755668613084
>500	0.07411487367013597	12.666335605686873
>1k	0.024625264477496786	8.670757639507007
>5k	0.0	0.0
>10k+	2.3908023764559992E-4	1.7594033070067059
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29319	1.7516649450136486	No Hit
GAGTATGGGGTTTTTGTCTTTGAGTTTGTTTATAAAGTGGATTACATTGC	4996	0.29848623982019135	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3951	0.236052668840988	No Hit
TCAAATGGGTAATTATAGCCTTTATTCTAGTCACAATTCTATCTCTAGGC	2948	0.17612838970975261	No Hit
AGGAAAGGAGAGGAAAGGAGAGGAAAGAGGGAAGAGGGAAGAGGGAAGAG	2487	0.14858592442610405	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2208	0.13191705715031674	No Hit
TGTATAGGGACATTTCTATTATTTGACCTAGAAATTGCTCTTCTACTTCC	2100	0.12546459239839908	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2055	0.12277606541843337	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1964	0.11733926641450275	No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC	1809	0.1080787845946209	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC	1776	0.10610719814264608	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTG	1690	0.10096912435871162	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12498663204640517	0.0
2	0.0	0.0	0.0	0.41355519456272305	0.0
3	0.0	0.0	0.0	0.5894446040964787	0.0
4	0.0	0.0	0.0	0.8631366506569864	0.0
5	0.0	0.0	0.0	1.375091932686454	0.0
6	0.0	0.0	0.0	1.8499455423923947	0.0
7	0.0	0.0	0.0	2.126385860976867	0.0
8	0.0	0.0	0.0	2.761057463380769	0.0
9	0.0	0.0	0.0	3.009895571637594	0.0
10	0.0	0.0	0.0	3.4728001725436872	0.0
11	0.0	0.0	0.0	4.095283785971744	0.0
12	0.0	0.0	0.0	4.607418303133209	0.0
13	0.0	0.0	0.0	4.793225389970838	0.0
14	0.0	0.0	0.0	4.867130009397895	0.0
15	0.0	0.0	0.0	4.981601513700435	0.0
16	0.0	0.0	0.0	5.256129990876932	0.0
17	0.0	0.0	0.0	5.592673823724637	0.0
18	0.0	0.0	0.0	6.064360946098619	0.0
19	0.0	0.0	0.0	6.287448940391772	0.0
20	0.0	0.0	0.0	6.4886702485812044	0.0
21	0.0	0.0	0.0	6.786559037961403	0.0
22	0.0	0.0	0.0	7.074410659949731	0.0
23	0.0	0.0	0.0	7.393807665169655	0.0
24	0.0	0.0	0.0	7.6472461418144215	0.0
25	0.0	0.0	0.0	7.8543224643157785	0.0
26	0.0	0.0	0.0	8.061817002125132	0.0
27	0.0	0.0	0.0	8.283590605450302	0.0
28	0.0	0.0	0.0	8.499628684551544	0.0
29	0.0	0.0	0.0	8.73185767057658	0.0
30	0.0	0.0	0.0	9.077004789760178	0.0
31	0.0	0.0	0.0	9.398492871520075	0.0
32	0.0	0.0	0.0	9.65276777878083	0.0
33	0.0	0.0	0.0	9.877349399173966	0.0
34	0.0	0.0	0.0	10.136822125262654	0.0
35	0.0	0.0	0.0	10.528809358941652	0.0
36	0.0	0.0	0.0	10.796825626322233	0.0
37	0.0	0.0	0.0	11.070995633234734	0.0
38	0.0	0.0	0.0	11.349885498623175	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	65	0.0	44.000004	1
TGCACAT	145	0.0	44.000004	12
TCACGAG	80	0.0	44.0	1
TGTCACG	25	4.4437133E-5	44.0	1
CTAGACG	35	1.4466059E-7	44.0	1
AATCCGT	35	1.4466059E-7	44.0	20
GTACCGT	25	4.4437133E-5	44.0	9
GTACCGG	35	1.4466059E-7	44.0	2
GGTCGTT	30	2.5284116E-6	44.0	8
GGTCGGA	80	0.0	44.0	8
GTCTGCA	30	2.5284116E-6	44.0	34
CTCCGAA	20	7.857513E-4	44.0	44
CGACGTC	35	1.4466059E-7	44.0	18
TGGACCG	20	7.857513E-4	44.0	17
CATTGCG	75	0.0	44.0	1
GACCGTT	25	4.4437133E-5	44.0	40
GACCGGA	20	7.857513E-4	44.0	41
CACGCTA	40	8.3164196E-9	44.0	26
TCGGGTA	50	2.7284841E-11	44.0	5
ATAGCCG	25	4.4437133E-5	44.0	2
>>END_MODULE