FastQCFastQC Report
Thu 26 May 2016
SRR1546208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546208_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1719699
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304201.768914211149742No Hit
GAGTATGGGGTTTTTGTCTTTGAGTTTGTTTATAAAGTGGATTACATTGC52000.3023784976324345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40180.23364553913213884No Hit
TCAAATGGGTAATTATAGCCTTTATTCTAGTCACAATTCTATCTCTAGGC29690.17264649220590347No Hit
AGGAAAGGAGAGGAAAGGAGAGGAAAGAGGGAAGAGGGAAGAGGGAAGAG25330.1472932181736455No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21550.12531262738421084No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA20970.12193994414138754No Hit
TGTATAGGGACATTTCTATTATTTGACCTAGAAATTGCTCTTCTACTTCC20430.11879985974289688No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC19490.11333378690107979No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC19480.11327563718999663No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC18600.1081584626146785No Hit
TTTGCAGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG17580.10222719208419612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG454.802132E-1044.01
CTATGCG700.044.01
CAGCGAT950.044.021
TCGTTAT207.8575726E-444.021
TCGTTAG302.5284535E-644.01
TCTTGCG207.8575726E-444.01
ACACGGA700.044.023
CTCGTTA207.8575726E-444.020
GGTCGTT254.4437656E-544.08
CCTAGGT408.3164196E-944.011
ACGTTAG254.4437656E-544.01
CAGCCGA207.8575726E-444.036
TAGACCG207.8575726E-444.011
CATTGCG351.4466423E-744.01
TAGCGCG207.8575726E-444.01
CACGCTA600.044.026
ACTTCGT207.8575726E-444.037
ATTCGCG454.802132E-1044.01
CTAAGCG408.3164196E-944.01
TCGATTG207.8575726E-444.01
Produced by FastQC (version 0.11.5)