##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546208_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1719699
Sequences flagged as poor quality	0
Sequence length	50
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845773010276798	31.0	31.0	34.0	30.0	34.0
2	32.227104278132394	33.0	31.0	34.0	30.0	34.0
3	32.205063211643434	33.0	31.0	34.0	30.0	34.0
4	35.927332632047815	37.0	35.0	37.0	35.0	37.0
5	35.859218967970556	37.0	35.0	37.0	35.0	37.0
6	35.9581467454479	37.0	35.0	37.0	35.0	37.0
7	36.16301806304475	37.0	35.0	37.0	35.0	37.0
8	36.06493578236657	37.0	35.0	37.0	35.0	37.0
9	37.84094251377712	39.0	38.0	39.0	35.0	39.0
10	37.57335091780597	39.0	37.0	39.0	35.0	39.0
11	37.35530694615744	39.0	37.0	39.0	34.0	39.0
12	36.73798670581305	39.0	35.0	39.0	33.0	39.0
13	36.50002587662143	39.0	35.0	39.0	33.0	39.0
14	37.648072133553605	40.0	35.0	41.0	33.0	41.0
15	37.87418088863225	40.0	35.0	41.0	33.0	41.0
16	37.97303074549674	40.0	35.0	41.0	34.0	41.0
17	37.89811007624009	40.0	35.0	41.0	34.0	41.0
18	37.63375451169071	39.0	36.0	41.0	34.0	41.0
19	37.30766023589012	37.0	36.0	41.0	34.0	41.0
20	36.950987353019336	37.0	35.0	41.0	34.0	41.0
21	36.8415106364544	37.0	35.0	41.0	33.0	41.0
22	36.73622535106434	37.0	35.0	41.0	33.0	41.0
23	36.71026732003682	37.0	35.0	41.0	33.0	41.0
24	36.59806512651342	37.0	35.0	41.0	33.0	41.0
25	36.57101039193487	37.0	35.0	40.0	33.0	41.0
26	36.49727306929876	37.0	35.0	40.0	33.0	41.0
27	36.41007350704978	37.0	35.0	41.0	32.0	41.0
28	36.32310886963358	37.0	35.0	41.0	32.0	41.0
29	36.17990357614908	36.0	35.0	41.0	32.0	41.0
30	35.89931435675662	36.0	35.0	40.0	31.0	41.0
31	35.4829147426381	36.0	35.0	40.0	30.0	41.0
32	34.956843610422524	36.0	35.0	41.0	24.0	41.0
33	34.3274904503637	36.0	34.0	41.0	21.0	41.0
34	33.757738999673784	36.0	34.0	40.0	16.0	41.0
35	33.35715029199877	36.0	33.0	40.0	15.0	41.0
36	33.03511137704912	36.0	33.0	40.0	10.0	41.0
37	32.91274577702261	36.0	33.0	40.0	10.0	41.0
38	32.86829264888797	36.0	33.0	40.0	10.0	41.0
39	32.806046290659005	36.0	33.0	40.0	10.0	41.0
40	32.631180223981055	36.0	32.0	40.0	10.0	41.0
41	32.53871229790795	36.0	32.0	40.0	10.0	41.0
42	32.4277638121555	36.0	32.0	40.0	9.0	41.0
43	32.28074389762394	35.0	32.0	40.0	8.0	41.0
44	32.11861145467899	35.0	31.0	40.0	8.0	41.0
45	32.034629897441356	35.0	31.0	40.0	8.0	41.0
46	31.943366833381887	35.0	31.0	40.0	8.0	41.0
47	31.929710373734007	35.0	31.0	40.0	8.0	41.0
48	31.85435358164423	35.0	31.0	40.0	8.0	41.0
49	31.704289529737473	35.0	30.0	40.0	8.0	41.0
50	31.464581883224913	35.0	30.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	1.0
11	0.0
12	1.0
13	9.0
14	9.0
15	53.0
16	124.0
17	350.0
18	871.0
19	1776.0
20	3302.0
21	5767.0
22	9293.0
23	14739.0
24	23534.0
25	39215.0
26	58536.0
27	66737.0
28	57993.0
29	46413.0
30	42371.0
31	42647.0
32	49725.0
33	63665.0
34	118890.0
35	242326.0
36	82307.0
37	103303.0
38	178539.0
39	467198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.35449575768783	23.123930408751765	26.938667755229257	23.58290607833115
2	38.568609971861356	25.38822200861895	26.168242233088463	9.874925786431229
3	23.49184363077492	23.626576511354603	42.99671047084402	9.88486938702645
4	21.050369861237346	27.12079265034172	42.00706053791971	9.82177695050122
5	19.731534413871266	29.58430516037981	40.477606837010434	10.206553588738494
6	15.42275712203124	38.281699297377045	37.83010864110521	8.465434939486503
7	74.51117899120719	4.542364681261081	19.662161808549055	1.2842945189826824
8	74.61392953069112	4.468223799630051	18.99937140162319	1.9184752680556305
9	71.27084449080915	6.128805099031866	20.60459417607384	1.995756234085151
10	39.369157044343225	27.90703489389713	25.648500115427176	7.075307946332468
11	35.056251123016295	18.865220018154343	35.92361221353272	10.154916645296648
12	34.43009503407282	20.709089206890276	35.74177806697568	9.119037692061227
13	17.480093900153456	38.73660448718061	32.645829299197125	11.13747231346881
14	10.389376280383951	40.63740224306696	35.23256104702044	13.740660429528656
15	9.203587371976143	27.268667365626193	52.10795610162011	11.419789160777555
16	10.732226976930265	21.59465115697573	49.6113564059757	18.061765460118313
17	12.284359065161985	22.7201388149903	37.10277205487705	27.89273006497067
18	14.19132069042315	27.513768397841716	43.94542300716579	14.349487904569347
19	17.54929205634242	27.468644222041185	37.0791051224662	17.9029585991502
20	20.886794723960413	22.588662318231272	41.21180508914642	15.3127378686619
21	16.002393442108183	28.809402110485614	39.578379704820435	15.609824742585769
22	19.47660608048269	25.27465562287354	33.58110925225868	21.66762904438509
23	14.332682638066313	28.873076044121674	34.09468750054515	22.69955381726686
24	11.853469706035765	24.7429928144402	51.58216641400617	11.82137106551786
25	12.353731670484196	30.40409978723021	43.304496891607194	13.937671650678404
26	12.321574880255207	32.34536974203044	37.675023361646424	17.658032016067928
27	11.520737059217922	37.89180548456445	34.43067653118366	16.156780925033974
28	10.672507223647859	32.4628903081295	41.14092059133604	15.723681876886594
29	11.352742543898671	24.19597848228091	41.435507027683336	23.015771946137086
30	12.49677995974877	35.46178720811026	36.99507878995103	15.046354042189941
31	18.567609796830727	35.20959191114259	33.47713756884199	12.745660723184695
32	19.231679497400417	27.98355991368257	34.824117476372315	17.960643112544695
33	19.18260114124623	35.53557919147479	30.20546037417013	15.076359293108851
34	15.472300675874093	30.598203522825795	32.13690302779731	21.792592773502804
35	18.9910559929383	29.181792860262174	33.12905339829819	18.698097748501336
36	24.661815817768108	32.09224404968543	28.787654118540512	14.458286014005939
37	15.817768109419147	35.42044276353013	34.75125588838512	14.010533238665603
38	15.031874764130235	30.631930355254028	37.14998962027657	17.186205260339165
39	16.03745771789133	32.88121932966176	34.72828675250727	16.35303619993964
40	19.622445555879256	28.73886651094174	32.16464043998397	19.47404749319503
41	14.143056430224126	30.023102880213337	34.74811580398663	21.085724885575907
42	16.836202149329623	28.929597563294507	30.97687444139934	23.257325845976535
43	17.142767426160045	28.676588170371677	33.00065883622657	21.17998556724171
44	15.461135931346126	30.21906740656359	31.851504245801156	22.468292416289128
45	14.794856541755271	39.955538730905815	26.06636393927077	19.183240788068147
46	18.734150569372897	36.155280662488025	29.73049353404288	15.3800752340962
47	16.793229512839165	32.362465757088884	32.20278665045453	18.641518079617423
48	17.955235189413962	28.557672011206613	34.8131271809776	18.673965618401827
49	20.135384157343815	28.862260197860206	32.597216140731604	18.405139504064376
50	17.593776585321034	32.00856661543677	30.287044418819804	20.11061238042239
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5044.0
1	5447.0
2	5850.0
3	24404.5
4	42959.0
5	33321.5
6	23684.0
7	25999.5
8	28315.0
9	31237.5
10	34160.0
11	34416.5
12	34673.0
13	33001.0
14	31329.0
15	28722.5
16	26116.0
17	24295.5
18	22475.0
19	20801.0
20	19127.0
21	17905.0
22	16683.0
23	15773.0
24	14863.0
25	18178.5
26	21494.0
27	25681.5
28	29869.0
29	37603.0
30	45337.0
31	53531.5
32	61726.0
33	82082.5
34	102439.0
35	110194.5
36	117950.0
37	106373.0
38	94796.0
39	111473.5
40	128151.0
41	137899.0
42	147647.0
43	154830.5
44	162014.0
45	148922.5
46	135831.0
47	125732.5
48	115634.0
49	105509.5
50	95385.0
51	77762.5
52	60140.0
53	48665.5
54	37191.0
55	29230.0
56	21269.0
57	16653.0
58	12037.0
59	9979.0
60	7921.0
61	6998.5
62	6076.0
63	5128.5
64	4181.0
65	3336.0
66	2491.0
67	1978.5
68	1466.0
69	1335.0
70	1204.0
71	1041.5
72	879.0
73	712.0
74	545.0
75	441.0
76	337.0
77	275.0
78	213.0
79	150.0
80	87.0
81	69.0
82	51.0
83	45.0
84	39.0
85	25.0
86	11.0
87	6.5
88	2.0
89	3.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1719699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.13851560413985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38862568261055	19.957122055135233
2	8.994635318225361	4.522235606015113
3	3.6843399886366446	2.778565148858961
4	1.8363758788405662	1.8465505474119837
5	1.0951734532638844	1.376551747205694
6	0.7410966641918972	1.11780420341784
7	0.501746499014906	0.8829213536366127
8	0.3759369257815547	0.7560397019945582
9	0.2892260062377461	0.6543641223837566
>10	2.062493133143664	10.724490518632933
>50	0.47719479226115596	8.66305351813217
>100	0.4539547062071902	23.383569938506835
>500	0.07387799204682688	12.665112411301504
>1k	0.0248583180786493	8.591340496858956
>5k	2.3232072970700277E-4	0.30369031102304156
>10k+	2.3232072970700277E-4	1.7765883194847933
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30420	1.768914211149742	No Hit
GAGTATGGGGTTTTTGTCTTTGAGTTTGTTTATAAAGTGGATTACATTGC	5200	0.3023784976324345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4018	0.23364553913213884	No Hit
TCAAATGGGTAATTATAGCCTTTATTCTAGTCACAATTCTATCTCTAGGC	2969	0.17264649220590347	No Hit
AGGAAAGGAGAGGAAAGGAGAGGAAAGAGGGAAGAGGGAAGAGGGAAGAG	2533	0.1472932181736455	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2155	0.12531262738421084	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2097	0.12193994414138754	No Hit
TGTATAGGGACATTTCTATTATTTGACCTAGAAATTGCTCTTCTACTTCC	2043	0.11879985974289688	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC	1949	0.11333378690107979	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	1948	0.11327563718999663	No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC	1860	0.1081584626146785	No Hit
TTTGCAGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG	1758	0.10222719208419612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12665007073912354	0.0
2	0.0	0.0	0.0	0.4186197700876723	0.0
3	0.0	0.0	0.0	0.5940574484255675	0.0
4	0.0	0.0	0.0	0.8789910327330539	0.0
5	0.0	0.0	0.0	1.4104793920331407	0.0
6	0.0	0.0	0.0	1.9002162587755183	0.0
7	0.0	0.0	0.0	2.188871424592327	0.0
8	0.0	0.0	0.0	2.84945214249703	0.0
9	0.0	0.0	0.0	3.105717919240518	0.0
10	0.0	0.0	0.0	3.583882993477347	0.0
11	5.814971108316048E-5	0.0	0.0	4.234403811364663	0.0
12	5.814971108316048E-5	0.0	0.0	4.7581001093796065	0.0
13	5.814971108316048E-5	0.0	0.0	4.958832912038677	0.0
14	5.814971108316048E-5	0.0	0.0	5.035997578646031	0.0
15	5.814971108316048E-5	0.0	0.0	5.1568908279879215	0.0
16	5.814971108316048E-5	0.0	0.0	5.440661418073744	0.0
17	5.814971108316048E-5	0.0	0.0	5.786361450463134	0.0
18	5.814971108316048E-5	0.0	0.0	6.286623414911563	0.0
19	5.814971108316048E-5	0.0	0.0	6.515500677734883	0.0
20	5.814971108316048E-5	0.0	0.0	6.734434339962982	0.0
21	5.814971108316048E-5	0.0	0.0	7.046581989057388	0.0
22	5.814971108316048E-5	0.0	0.0	7.3472159953573275	0.0
23	5.814971108316048E-5	0.0	0.0	7.691927482658302	0.0
24	5.814971108316048E-5	0.0	0.0	7.960637297573587	0.0
25	5.814971108316048E-5	0.0	0.0	8.17393043782662	0.0
26	5.814971108316048E-5	0.0	0.0	8.387979524323734	0.0
27	5.814971108316048E-5	0.0	0.0	8.6125537085269	0.0
28	5.814971108316048E-5	0.0	0.0	8.836953443596816	0.0
29	5.814971108316048E-5	0.0	0.0	9.076472103548353	0.0
30	5.814971108316048E-5	0.0	0.0	9.442989732505515	0.0
31	5.814971108316048E-5	0.0	0.0	9.773512690302198	0.0
32	5.814971108316048E-5	0.0	0.0	10.036233084975917	0.0
33	5.814971108316048E-5	0.0	0.0	10.26702928826498	0.0
34	5.814971108316048E-5	0.0	0.0	10.532308270226359	0.0
35	5.814971108316048E-5	0.0	0.0	10.933832025255583	0.0
36	5.814971108316048E-5	0.0	0.0	11.212659889899337	0.0
37	5.814971108316048E-5	0.0	0.0	11.50875821873479	0.0
38	5.814971108316048E-5	0.0	0.0	11.808519979368482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	45	4.802132E-10	44.0	1
CTATGCG	70	0.0	44.0	1
CAGCGAT	95	0.0	44.0	21
TCGTTAT	20	7.8575726E-4	44.0	21
TCGTTAG	30	2.5284535E-6	44.0	1
TCTTGCG	20	7.8575726E-4	44.0	1
ACACGGA	70	0.0	44.0	23
CTCGTTA	20	7.8575726E-4	44.0	20
GGTCGTT	25	4.4437656E-5	44.0	8
CCTAGGT	40	8.3164196E-9	44.0	11
ACGTTAG	25	4.4437656E-5	44.0	1
CAGCCGA	20	7.8575726E-4	44.0	36
TAGACCG	20	7.8575726E-4	44.0	11
CATTGCG	35	1.4466423E-7	44.0	1
TAGCGCG	20	7.8575726E-4	44.0	1
CACGCTA	60	0.0	44.0	26
ACTTCGT	20	7.8575726E-4	44.0	37
ATTCGCG	45	4.802132E-10	44.0	1
CTAAGCG	40	8.3164196E-9	44.0	1
TCGATTG	20	7.8575726E-4	44.0	1
>>END_MODULE