FastQCFastQC Report
Thu 26 May 2016
SRR1546207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546207_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1166441
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165341.4174741800056754No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC18890.16194561062239754No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG15970.13691219701639432No Hit
GGAATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14620.12533852976704352No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA14440.12379537413379674No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC14340.12293806544865964TruSeq Adapter, Index 21 (95% over 21bp)
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13970.1197660233136524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13920.11933736897108384No Hit
AAATACGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13650.11702263552121367No Hit
TGTGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13130.11256463035850077No Hit
GCGGAAGGGATTTACTTATAGGTGTAATATTTGTTAATGGTTTTAAAATG12600.10802089432727416No Hit
GGAAAAGGGCATAATATGAGTAAGGGAATCAATTCTCTACAAGTGTCTTA12350.10587762261443143No Hit
AAAGCAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA12130.1039915435071298No Hit
GCGATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11820.10133388658320482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATT950.044.00000413
TTACGAT950.044.00000412
TCGACAG650.044.0000041
ACGTTCC254.44288E-544.025
CTATACG351.4461148E-744.01
CCGTGAT207.856531E-444.033
AGGAACG302.5277495E-644.01
CGCGACA207.856531E-444.028
TCGATAG302.5277495E-644.01
TAGCCGT207.856531E-444.044
AGTATCG302.5277495E-644.01
CGTTAGT207.856531E-444.035
ACTAGTG408.312782E-944.027
GTTCGGT302.5277495E-644.09
GTATGCG254.44288E-544.07
CGCTAGC207.856531E-444.021
AGGCACG207.856531E-444.010
CCGAGTA351.4461148E-744.025
ACTACGG600.044.02
GTATCGG302.5277495E-644.02
Produced by FastQC (version 0.11.5)