##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546207_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1166441
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.931760800589142	31.0	31.0	34.0	30.0	34.0
2	32.278428141671974	33.0	31.0	34.0	30.0	34.0
3	32.18419791485382	33.0	31.0	34.0	30.0	34.0
4	35.94229712432948	37.0	35.0	37.0	35.0	37.0
5	35.93800543705168	37.0	35.0	37.0	35.0	37.0
6	35.99996913688734	37.0	35.0	37.0	35.0	37.0
7	36.21445405296967	37.0	35.0	37.0	35.0	37.0
8	36.11902445130101	37.0	36.0	37.0	35.0	37.0
9	37.906940856845736	39.0	38.0	39.0	35.0	39.0
10	37.55506279357464	39.0	37.0	39.0	35.0	39.0
11	37.376754589387716	39.0	37.0	39.0	35.0	39.0
12	36.70319887589685	39.0	35.0	39.0	33.0	39.0
13	36.45686579947036	39.0	35.0	39.0	33.0	39.0
14	37.48269222361011	40.0	35.0	41.0	33.0	41.0
15	37.70859820599585	40.0	35.0	41.0	33.0	41.0
16	37.85019473766783	40.0	35.0	41.0	33.0	41.0
17	37.779162426560795	40.0	35.0	41.0	34.0	41.0
18	37.59819485083258	39.0	36.0	41.0	34.0	41.0
19	37.38364049274674	38.0	35.0	41.0	34.0	41.0
20	37.08437374886514	38.0	35.0	41.0	33.0	41.0
21	36.97265956872229	38.0	35.0	41.0	33.0	41.0
22	36.889655799136	38.0	35.0	41.0	33.0	41.0
23	36.85491593659688	38.0	35.0	41.0	33.0	41.0
24	36.80431843530877	38.0	35.0	41.0	33.0	41.0
25	36.75987726768864	38.0	35.0	41.0	33.0	41.0
26	36.72371684465824	38.0	35.0	41.0	33.0	41.0
27	36.65191895689538	38.0	35.0	41.0	33.0	41.0
28	36.5388510863387	37.0	35.0	41.0	32.0	41.0
29	36.452366643490755	37.0	35.0	41.0	32.0	41.0
30	36.23751137005644	37.0	35.0	41.0	32.0	41.0
31	35.890946048707136	37.0	35.0	41.0	31.0	41.0
32	35.52008631383842	37.0	35.0	41.0	29.0	41.0
33	35.114624743128886	37.0	35.0	41.0	25.0	41.0
34	34.658336769712314	37.0	34.0	40.0	23.0	41.0
35	34.378612377308414	37.0	34.0	41.0	21.0	41.0
36	34.16358992868049	37.0	34.0	40.0	18.0	41.0
37	34.1229586408571	37.0	34.0	40.0	18.0	41.0
38	34.03496104817989	37.0	34.0	40.0	18.0	41.0
39	33.97972550690519	36.0	34.0	40.0	18.0	41.0
40	33.79073523650146	36.0	34.0	40.0	18.0	41.0
41	33.677770242987	36.0	34.0	40.0	17.0	41.0
42	33.60965878257023	36.0	33.0	40.0	16.0	41.0
43	33.50673030183267	36.0	33.0	40.0	15.0	41.0
44	33.35484606593904	36.0	33.0	40.0	15.0	41.0
45	33.322482663075114	35.0	33.0	40.0	15.0	41.0
46	33.25125745751392	35.0	33.0	40.0	15.0	41.0
47	33.213523015737614	35.0	33.0	40.0	15.0	41.0
48	33.13326006201771	35.0	33.0	40.0	15.0	41.0
49	33.02898303471843	35.0	33.0	40.0	14.0	41.0
50	32.90248628091776	35.0	33.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	6.0
14	10.0
15	22.0
16	71.0
17	170.0
18	381.0
19	771.0
20	1486.0
21	2639.0
22	4252.0
23	6731.0
24	10738.0
25	18030.0
26	27792.0
27	34121.0
28	32549.0
29	27981.0
30	27333.0
31	29178.0
32	34960.0
33	45883.0
34	89402.0
35	177038.0
36	63157.0
37	80000.0
38	135554.0
39	316182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.943013834390253	22.247674764518738	26.62303536998442	20.186276031106587
2	36.39215356799015	23.26950098633364	28.86944131764916	11.468904128027049
3	25.559886869545913	23.16362336371921	39.586742921416516	11.689746845318366
4	23.113127882164637	25.76581241571584	38.8656605863477	12.255399115771823
5	19.218545987323836	29.87360698055024	39.14154252122482	11.766304510901108
6	16.11174504325551	40.54041310276302	34.90052218672012	8.447319667261354
7	78.30486068305213	3.6590791990336413	16.55823140647491	1.477828711439327
8	78.64384053715533	2.905504864798134	16.051219050084832	2.3994355479617058
9	75.6139401821438	3.5055352135255875	17.87694362595279	3.003580978377818
10	42.91130027150966	24.037135183005397	23.294277207334105	9.757287338150837
11	36.820722179690186	19.78308375648661	31.31902942369138	12.07716464013182
12	35.818356865027894	18.22586826080359	34.362132332454024	11.593642541714496
13	18.710676322248617	35.28914021369276	32.561526901060574	13.438656562998041
14	12.395397624054711	36.75839583828072	35.865851766184484	14.980354771480084
15	10.87153143622352	22.55999231851418	53.517151746209194	13.051324499053102
16	13.699192672411206	18.88848214354605	48.36164023726875	19.05068494677399
17	13.189265466491662	19.985837260521535	36.40878535648181	30.41611191650499
18	15.613562966322341	23.948318003225193	44.22881225882835	16.20930677162411
19	20.27989413952356	24.799196873223764	35.2080388120788	19.712870175173883
20	21.63418466943463	21.959447584575646	38.80230547451607	17.60406227147365
21	17.505214580077347	26.15357313400335	38.47018408989396	17.871028196025346
22	18.678698708293005	23.12710201373237	33.255175358205	24.939023919769625
23	13.793496627776285	26.259793680091835	34.88937717381334	25.057332518318542
24	14.5314679439423	22.358267584901423	48.001570589511175	15.108693881645108
25	13.641581528769994	27.026570568078455	42.14169426486209	17.190153638289463
26	14.17559910874189	27.39135541360429	37.39100391704338	21.04204156061044
27	14.992785747414572	32.15216200390761	34.613152315462166	18.241899933215652
28	12.41228660515191	27.930688307424035	41.03859517969619	18.618429907727865
29	12.769012749037456	23.234351330243022	40.093841008675106	23.902794912044413
30	15.531861448628778	30.208643214701812	38.366106815518314	15.893388521151092
31	20.938993056656958	30.42202734643244	32.76968144981186	15.869298147098739
32	21.7940727392127	24.207310957005113	35.679644319772706	18.318971984009476
33	23.061775091924925	28.223459223398358	31.08764180957288	17.62712387510384
34	17.742517624123295	25.198359797023596	35.08938729005582	21.969735288797292
35	19.33145354115639	24.58375520064881	35.00185607330332	21.082935184891475
36	26.374673043900206	26.15905990958823	31.82732774310917	15.638939303402402
37	17.125855486904182	30.309548446942454	36.93800200781694	15.626594058336426
38	17.551252056469206	28.29281549602595	35.14751281890812	19.00841962859673
39	17.41965517330066	29.294666425477157	34.742091541706785	18.543586859515397
40	20.144182174666355	24.50196795208673	33.78267739216986	21.571172481077053
41	15.625308095308721	25.919527862960923	35.152570940150426	23.302593101579934
42	18.16765700108278	25.13954842122319	32.5767012647875	24.116093312906525
43	18.863020075597483	23.822122164773017	34.78581428464877	22.529043474980732
44	16.292808637556462	26.63015103207106	34.315580470851074	22.7614598595214
45	16.63478907205765	34.14703358335312	29.382540565703707	19.835636778885515
46	20.788020997204313	29.723663691519757	32.9537456245108	16.534569686765128
47	17.89194652794269	28.290672224313102	33.52557051749724	20.291810730246965
48	18.00614004480295	26.795097223091435	35.00631407846603	20.192448653639573
49	20.131579736994844	24.774077728749248	35.05080839922465	20.043534135031262
50	18.44653951635788	27.866990272118347	34.24493823519578	19.44153197632799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2155.0
1	4011.0
2	5867.0
3	17750.5
4	29634.0
5	21832.5
6	14031.0
7	14680.5
8	15330.0
9	16205.5
10	17081.0
11	16943.0
12	16805.0
13	15942.0
14	15079.0
15	14066.5
16	13054.0
17	11266.5
18	9479.0
19	9331.5
20	9184.0
21	8419.5
22	7655.0
23	10187.0
24	12719.0
25	12188.5
26	11658.0
27	13233.0
28	14808.0
29	18782.0
30	22756.0
31	29821.5
32	36887.0
33	51753.0
34	66619.0
35	67861.0
36	69103.0
37	63950.0
38	58797.0
39	63445.5
40	68094.0
41	76466.5
42	84839.0
43	88357.5
44	91876.0
45	90340.0
46	88804.0
47	85844.5
48	82885.0
49	78815.5
50	74746.0
51	63317.5
52	51889.0
53	43977.5
54	36066.0
55	31943.5
56	27821.0
57	25770.5
58	23720.0
59	22751.0
60	21782.0
61	19993.5
62	18205.0
63	15781.0
64	13357.0
65	12090.0
66	10823.0
67	9101.0
68	7379.0
69	6325.5
70	5272.0
71	4821.5
72	4371.0
73	3681.5
74	2992.0
75	2192.5
76	1393.0
77	1046.0
78	699.0
79	540.5
80	382.0
81	280.5
82	179.0
83	116.5
84	54.0
85	68.5
86	83.0
87	49.0
88	15.0
89	11.0
90	7.0
91	5.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1166441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.315642052190235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7134114693799	19.926805494655873
2	8.796393833748233	4.453727152905273
3	3.3569061716462603	2.54946705132555
4	1.7641972127020948	1.7864714058495181
5	1.0648399153246304	1.347855306962145
6	0.7090650417915684	1.0770262073829993
7	0.5348863784434953	0.9478694466687523
8	0.4127230831479546	0.8358679871719973
9	0.34597701051619906	0.7882767140863457
>10	3.0973080630703533	18.216440071323817
>50	0.7238001560989291	12.449274295305834
>100	0.4137945209508287	21.123414224938212
>500	0.05648839677453708	10.028457244799283
>1k	0.009868454858202261	3.044693065113615
>5k	0.0	0.0
>10k+	3.4029154683456076E-4	1.424354331510865
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16534	1.4174741800056754	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	1889	0.16194561062239754	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1597	0.13691219701639432	No Hit
GGAATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1462	0.12533852976704352	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1444	0.12379537413379674	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1434	0.12293806544865964	TruSeq Adapter, Index 21 (95% over 21bp)
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1397	0.1197660233136524	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1392	0.11933736897108384	No Hit
AAATACGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1365	0.11702263552121367	No Hit
TGTGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1313	0.11256463035850077	No Hit
GCGGAAGGGATTTACTTATAGGTGTAATATTTGTTAATGGTTTTAAAATG	1260	0.10802089432727416	No Hit
GGAAAAGGGCATAATATGAGTAAGGGAATCAATTCTCTACAAGTGTCTTA	1235	0.10587762261443143	No Hit
AAAGCAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1213	0.1039915435071298	No Hit
GCGATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1182	0.10133388658320482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14634259255290238	0.0
2	0.0	0.0	0.0	0.5095842824454901	0.0
3	0.0	0.0	0.0	0.6956202671202402	0.0
4	0.0	0.0	0.0	0.9939636895479497	0.0
5	0.0	0.0	0.0	1.6025671251267746	0.0
6	0.0	0.0	0.0	2.146358024109235	0.0
7	0.0	0.0	0.0	2.460475926343467	0.0
8	0.0	0.0	0.0	3.3429037559550805	0.0
9	0.0	0.0	0.0	3.696200665100078	0.0
10	0.0	0.0	0.0	4.352641925309553	0.0
11	0.0	0.0	0.0	5.0233145096923035	0.0
12	0.0	0.0	0.0	5.5769644585538405	0.0
13	0.0	0.0	0.0	5.79326343981393	0.0
14	0.0	0.0	0.0	5.870764144950323	0.0
15	0.0	0.0	0.0	5.995502558637771	0.0
16	0.0	0.0	0.0	6.290931131536015	0.0
17	0.0	0.0	0.0	6.627253328715297	0.0
18	0.0	0.0	0.0	7.179017198469532	0.0
19	0.0	0.0	0.0	7.408947387823302	0.0
20	0.0	0.0	0.0	7.668368995945787	0.0
21	0.0	0.0	0.0	8.023380522461059	0.0
22	0.0	0.0	0.0	8.377620471159707	0.0
23	0.0	0.0	0.0	8.783213210098067	0.0
24	0.0	0.0	0.0	9.085243059871866	0.0
25	0.0	0.0	0.0	9.341492625859345	0.0
26	0.0	0.0	0.0	9.60091423398183	0.0
27	0.0	0.0	0.0	9.886569487869512	0.0
28	0.0	0.0	0.0	10.143333439068071	0.0
29	0.0	0.0	0.0	10.471511203738551	0.0
30	0.0	0.0	0.0	10.863044080240664	0.0
31	0.0	0.0	0.0	11.208796672956455	0.0
32	0.0	0.0	0.0	11.494280465107108	0.0
33	0.0	0.0	0.0	11.794681428379146	0.0
34	0.0	0.0	0.0	12.105713019346885	0.0
35	0.0	0.0	0.0	12.562144163313874	0.0
36	0.0	0.0	0.0	12.870003712146607	0.0
37	0.0	0.0	0.0	13.16311755159498	0.0
38	0.0	0.0	0.0	13.486322925891665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATT	95	0.0	44.000004	13
TTACGAT	95	0.0	44.000004	12
TCGACAG	65	0.0	44.000004	1
ACGTTCC	25	4.44288E-5	44.0	25
CTATACG	35	1.4461148E-7	44.0	1
CCGTGAT	20	7.856531E-4	44.0	33
AGGAACG	30	2.5277495E-6	44.0	1
CGCGACA	20	7.856531E-4	44.0	28
TCGATAG	30	2.5277495E-6	44.0	1
TAGCCGT	20	7.856531E-4	44.0	44
AGTATCG	30	2.5277495E-6	44.0	1
CGTTAGT	20	7.856531E-4	44.0	35
ACTAGTG	40	8.312782E-9	44.0	27
GTTCGGT	30	2.5277495E-6	44.0	9
GTATGCG	25	4.44288E-5	44.0	7
CGCTAGC	20	7.856531E-4	44.0	21
AGGCACG	20	7.856531E-4	44.0	10
CCGAGTA	35	1.4461148E-7	44.0	25
ACTACGG	60	0.0	44.0	2
GTATCGG	30	2.5277495E-6	44.0	2
>>END_MODULE