FastQCFastQC Report
Thu 26 May 2016
SRR1546206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546206_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1200822
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173311.4432613659643145No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC20070.1671355121741607No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG16920.1409034811154359No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC15430.12849531404321374TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14610.12166665833903775No Hit
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14550.12116700060458586No Hit
GGAATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14380.11975130369030547No Hit
TGTGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13970.11633697583821748No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA13860.115420936658389No Hit
AAATACGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13790.11483800263486178No Hit
GGAAAAGGGCATAATATGAGTAAGGGAATCAATTCTCTACAAGTGTCTTA13610.11333902943150607No Hit
AGCATGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12890.10734313661808327No Hit
GCGATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12750.10617726857102884No Hit
TGCAATGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT12580.10476157165674846No Hit
GCGGAAGGGATTTACTTATAGGTGTAATATTTGTTAATGGTTTTAAAATG12320.10259638814079024No Hit
TCGGAAGGGTTTCAACCATGAAAAATACTGAAAATTTTTGTTTTGTCTGG12060.10043120462483199No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTA207.856622E-444.0000049
AGATCGA207.856622E-444.00000415
ACCGGAC207.856622E-444.00000437
ACGTTAG800.044.0000041
ACGCACG207.856622E-444.00000411
TAGCGAT207.856622E-444.00000440
CAACCCG207.856622E-444.00000423
CGACCGG207.856622E-444.0000042
CGATTCA207.856622E-444.00000410
CTAAGCG207.856622E-444.0000041
CACGACA207.856622E-444.00000416
CGTTCTA207.856622E-444.00000431
TAGCCGT207.856622E-444.00000444
ATCGTAC408.312782E-944.00000434
CTTATCG408.312782E-944.00000431
AGCGATA207.856622E-444.00000441
CGAATAT408.312782E-944.00000414
GCGATTC207.856622E-444.0000049
TACTCGT207.856622E-444.00000440
ACGTACC207.856622E-444.00000415
Produced by FastQC (version 0.11.5)