##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546206_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1200822
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.866060082177043	31.0	31.0	34.0	30.0	34.0
2	32.244031171980524	33.0	31.0	34.0	30.0	34.0
3	32.27539718626075	34.0	31.0	34.0	30.0	34.0
4	35.98202148195153	37.0	35.0	37.0	35.0	37.0
5	35.902482632729914	37.0	35.0	37.0	35.0	37.0
6	35.98759100016489	37.0	35.0	37.0	35.0	37.0
7	36.1912681479853	37.0	35.0	37.0	35.0	37.0
8	36.097524862136105	37.0	35.0	37.0	35.0	37.0
9	37.877277398315485	39.0	38.0	39.0	35.0	39.0
10	37.54775645349602	39.0	37.0	39.0	35.0	39.0
11	37.36412890503338	39.0	37.0	39.0	34.0	39.0
12	36.678884963799796	39.0	35.0	39.0	33.0	39.0
13	36.38896439272432	39.0	35.0	39.0	33.0	39.0
14	37.39698639765094	40.0	35.0	41.0	33.0	41.0
15	37.662717705038716	40.0	35.0	41.0	33.0	41.0
16	37.79745124589656	40.0	35.0	41.0	33.0	41.0
17	37.742556348901005	40.0	35.0	41.0	33.0	41.0
18	37.57514685773578	39.0	36.0	41.0	33.0	41.0
19	37.36415888449745	38.0	35.0	41.0	34.0	41.0
20	37.06619882047464	38.0	35.0	41.0	33.0	41.0
21	36.94549816708888	38.0	35.0	41.0	33.0	41.0
22	36.879441749068555	38.0	35.0	41.0	33.0	41.0
23	36.830232957091056	38.0	35.0	41.0	33.0	41.0
24	36.78793193329236	38.0	35.0	41.0	33.0	41.0
25	36.72153241696105	38.0	35.0	41.0	33.0	41.0
26	36.70417763831775	38.0	35.0	41.0	33.0	41.0
27	36.62374523451436	38.0	35.0	41.0	33.0	41.0
28	36.50431204624832	37.0	35.0	41.0	32.0	41.0
29	36.41784211148696	37.0	35.0	41.0	32.0	41.0
30	36.21138436837433	37.0	35.0	40.0	31.0	41.0
31	35.8725389774671	37.0	35.0	40.0	31.0	41.0
32	35.50104928124235	37.0	35.0	41.0	29.0	41.0
33	35.09506071674237	37.0	35.0	41.0	25.0	41.0
34	34.70026615101988	37.0	34.0	41.0	23.0	41.0
35	34.39851701584415	37.0	34.0	41.0	21.0	41.0
36	34.17205547533273	37.0	34.0	40.0	19.0	41.0
37	34.079346480993856	37.0	34.0	40.0	18.0	41.0
38	34.00257407009532	37.0	34.0	40.0	18.0	41.0
39	33.945177553375935	36.0	34.0	40.0	18.0	41.0
40	33.77276065894862	36.0	34.0	40.0	18.0	41.0
41	33.688808999168906	36.0	34.0	40.0	18.0	41.0
42	33.604873994647	36.0	33.0	40.0	16.0	41.0
43	33.52207820975965	36.0	33.0	40.0	15.0	41.0
44	33.36886232930443	36.0	33.0	40.0	15.0	41.0
45	33.33562842786025	35.0	33.0	40.0	15.0	41.0
46	33.279654270158275	35.0	33.0	40.0	15.0	41.0
47	33.225309829433506	35.0	33.0	40.0	15.0	41.0
48	33.15033951743056	35.0	33.0	40.0	15.0	41.0
49	33.04860254059303	35.0	33.0	40.0	15.0	41.0
50	32.894514757391185	35.0	33.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	2.0
13	3.0
14	10.0
15	21.0
16	68.0
17	153.0
18	395.0
19	816.0
20	1533.0
21	2730.0
22	4498.0
23	6943.0
24	10941.0
25	18138.0
26	28295.0
27	35185.0
28	33639.0
29	29357.0
30	28548.0
31	30857.0
32	36244.0
33	48024.0
34	94105.0
35	179513.0
36	65709.0
37	82607.0
38	139982.0
39	322500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.10710829748289	22.161902430168666	26.780655251152965	19.95033402119548
2	36.405062532165466	23.096512222460948	29.044937551110824	11.453487694262764
3	25.542170280024852	23.21060073849413	39.56531442628466	11.681914555196357
4	23.128407041176793	25.66325400434036	38.87412122695954	12.334217727523313
5	19.18519147717147	29.829150365333078	39.191986822360015	11.793671335135432
6	16.212727614917117	40.43846631723936	34.86969759048385	8.479108477359675
7	78.27396566685154	3.6701526121273593	16.527178882465513	1.5287028385555894
8	78.62539160674937	2.9016790165403363	16.071907410090756	2.4010219666195325
9	75.59621659163473	3.5030170999531984	17.869093004625164	3.031673303786906
10	42.96740066387857	23.915201420360386	23.300872235851774	9.816525679909262
11	36.84834221891337	19.791775966796077	31.281322294228453	12.07855952006209
12	35.91556450498076	18.197118307292836	34.28243319992472	11.60488398780169
13	18.896888964392726	35.17107448064743	32.50648305910451	13.425553495855338
14	12.444891915704408	36.61466895176804	35.92122729263787	15.019211839889676
15	10.840490930379357	22.493675165844728	53.50401641542211	13.161817488353812
16	13.863420223813355	18.91920700986491	48.198900419879045	19.018472346442685
17	13.259583851728232	19.96265891197863	36.415638620878035	30.362118615415106
18	15.682840587530874	23.919864892548603	44.19114573184035	16.206148788080167
19	20.335403581879746	24.806091160888126	35.22062387264724	19.637881384584894
20	21.716707388772026	21.92531449290569	38.764029972801964	17.59394814552032
21	17.462121779914092	26.08504840850684	38.55184198823805	17.900987823341012
22	18.57602542258553	23.035720531435967	33.40170316666417	24.986550879314336
23	13.78830501106742	26.225285679309675	34.89393099060477	25.09247831901814
24	14.601081592442512	22.355519802268777	47.80100631067719	15.242392294611523
25	13.724098992190351	26.929886361175924	42.05227752322992	17.2937371234038
26	14.18803119862894	27.270070002048598	37.437355411543095	21.10454338777937
27	15.038365386376999	32.031142001062605	34.57306744879757	18.357425163762823
28	12.401754797963395	27.883982805111835	40.94661823317694	18.767644163747832
29	12.870267200301127	23.414461094150507	40.005096508891405	23.71017519665696
30	15.614970411934493	30.093552583147208	38.26320637030301	16.02827063461529
31	20.886276234112966	30.34005039881015	32.78962244196059	15.984050925116295
32	21.842454585275753	24.150373660709082	35.67031583365395	18.33685592036122
33	22.99974517455543	28.180196565352734	31.124013384165178	17.696044875926656
34	17.756503461795337	25.107634603629847	34.98403593538426	22.151825999190557
35	19.320681999497012	24.58141173296292	34.88751871634597	21.2103875511941
36	26.418986327698864	26.03841368662466	31.803214797863465	15.739385187813015
37	17.254930372694705	30.22188134461227	36.85808554473519	15.665102737957831
38	17.586370003214466	28.25939231626336	35.1570007877937	18.99723689272848
39	17.46603576550063	29.179512034256533	34.76060565179518	18.593846548447644
40	20.113472271494025	24.346239492614227	33.911187503226955	21.629100732664792
41	15.624297356310926	25.923409131411653	35.16965878373314	23.28263472854428
42	18.156229649356856	25.121208638749128	32.76205799027666	23.96050372161736
43	18.88964392724317	23.702597054351102	34.78533870965055	22.62242030875517
44	16.391938189007195	26.44871596289875	34.33589657751107	22.823449270582984
45	16.71871434733874	33.893366377364835	29.411103394174994	19.976815881121432
46	20.743707227216024	29.54692702165683	33.1404654478349	16.568900303292246
47	17.924971394594703	28.11407519182693	33.61780513681462	20.34314827676375
48	18.012578050701936	26.7248601374725	35.073391393562076	20.189170418263487
49	20.174680343964386	24.637206846643384	35.08754836270488	20.10056444668735
50	18.36633572669388	27.779554338611383	34.25486874824079	19.599241186453945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2297.0
1	4121.0
2	5945.0
3	18458.0
4	30971.0
5	22500.0
6	14029.0
7	14856.0
8	15683.0
9	16464.5
10	17246.0
11	17209.0
12	17172.0
13	16296.0
14	15420.0
15	14349.5
16	13279.0
17	11613.5
18	9948.0
19	9649.5
20	9351.0
21	8580.0
22	7809.0
23	10323.5
24	12838.0
25	12427.5
26	12017.0
27	13482.0
28	14947.0
29	19061.5
30	23176.0
31	30628.5
32	38081.0
33	52801.5
34	67522.0
35	68843.0
36	70164.0
37	65203.5
38	60243.0
39	65136.0
40	70029.0
41	78228.5
42	86428.0
43	90661.5
44	94895.0
45	93249.0
46	91603.0
47	89114.0
48	86625.0
49	82712.0
50	78799.0
51	66455.0
52	54111.0
53	45815.0
54	37519.0
55	33134.5
56	28750.0
57	26666.5
58	24583.0
59	23616.0
60	22649.0
61	20584.5
62	18520.0
63	16249.0
64	13978.0
65	12472.0
66	10966.0
67	9241.0
68	7516.0
69	6403.5
70	5291.0
71	4926.0
72	4561.0
73	3782.5
74	3004.0
75	2219.5
76	1435.0
77	1079.0
78	723.0
79	535.0
80	347.0
81	265.5
82	184.0
83	128.5
84	73.0
85	70.5
86	68.0
87	43.0
88	18.0
89	11.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1200822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.32441460984794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53163054298514	19.887675718579537
2	8.959530198266172	4.537897149006912
3	3.324690781740549	2.5258754341901137
4	1.7669964577713657	1.789926036429389
5	1.086985565639196	1.376363656958354
6	0.7443329776910215	1.1309878160898073
7	0.5381246048908943	0.9539383424212329
8	0.40491906644490194	0.8203470657666612
9	0.3300317712551992	0.7522075268720243
>10	3.1234696852216985	18.270444940158548
>50	0.7117209726349619	12.308525980135727
>100	0.4120837602643966	21.146190195405172
>500	0.055561855316546574	9.989956106992318
>1k	0.009591034548689587	3.0581190618024467
>5k	0.0	0.0
>10k+	3.3072532926515814E-4	1.4515449691917457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17331	1.4432613659643145	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2007	0.1671355121741607	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1692	0.1409034811154359	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1543	0.12849531404321374	TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1461	0.12166665833903775	No Hit
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1455	0.12116700060458586	No Hit
GGAATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1438	0.11975130369030547	No Hit
TGTGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1397	0.11633697583821748	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1386	0.115420936658389	No Hit
AAATACGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1379	0.11483800263486178	No Hit
GGAAAAGGGCATAATATGAGTAAGGGAATCAATTCTCTACAAGTGTCTTA	1361	0.11333902943150607	No Hit
AGCATGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1289	0.10734313661808327	No Hit
GCGATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1275	0.10617726857102884	No Hit
TGCAATGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1258	0.10476157165674846	No Hit
GCGGAAGGGATTTACTTATAGGTGTAATATTTGTTAATGGTTTTAAAATG	1232	0.10259638814079024	No Hit
TCGGAAGGGTTTCAACCATGAAAAATACTGAAAATTTTTGTTTTGTCTGG	1206	0.10043120462483199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1551437265473151	0.0
2	0.0	0.0	0.0	0.5234747531274411	0.0
3	0.0	0.0	0.0	0.7175917829620043	0.0
4	0.0	0.0	0.0	1.0173864236331447	0.0
5	0.0	0.0	0.0	1.6373783957988777	0.0
6	0.0	0.0	0.0	2.1872517325631944	0.0
7	0.0	0.0	0.0	2.526102952810658	0.0
8	0.0	0.0	0.0	3.421073231503087	0.0
9	0.0	0.0	0.0	3.7939844539823553	0.0
10	0.0	0.0	0.0	4.498668412137686	0.0
11	0.0	0.0	0.0	5.2041018568946935	0.0
12	0.0	0.0	0.0	5.764218177215274	0.0
13	0.0	0.0	0.0	5.986815697913596	0.0
14	0.0	0.0	0.0	6.071091302457816	0.0
15	0.0	0.0	0.0	6.197837814430448	0.0
16	0.0	0.0	0.0	6.50312869018056	0.0
17	0.0	0.0	0.0	6.870293848713631	0.0
18	0.0	0.0	0.0	7.454060635131602	0.0
19	0.0	0.0	0.0	7.696311360051698	0.0
20	0.0	0.0	0.0	7.977035730524591	0.0
21	0.0	0.0	0.0	8.350363334449236	0.0
22	0.0	0.0	0.0	8.724190596108333	0.0
23	0.0	0.0	0.0	9.144069645626079	0.0
24	0.0	0.0	0.0	9.448611034774514	0.0
25	0.0	0.0	0.0	9.70793339895505	0.0
26	0.0	0.0	0.0	9.985326717864929	0.0
27	0.0	0.0	0.0	10.287119989473878	0.0
28	0.0	0.0	0.0	10.561931743422422	0.0
29	0.0	0.0	0.0	10.896036215192593	0.0
30	0.0	0.0	0.0	11.311168516233048	0.0
31	0.0	0.0	0.0	11.660512548904	0.0
32	0.0	0.0	0.0	11.958641663793635	0.0
33	0.0	0.0	0.0	12.284751611812576	0.0
34	0.0	0.0	0.0	12.605698429908847	0.0
35	0.0	0.0	0.0	13.079956896192774	0.0
36	0.0	0.0	0.0	13.409814277220104	0.0
37	0.0	0.0	0.0	13.72443209734665	0.0
38	0.0	0.0	0.0	14.056371385600864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTA	20	7.856622E-4	44.000004	9
AGATCGA	20	7.856622E-4	44.000004	15
ACCGGAC	20	7.856622E-4	44.000004	37
ACGTTAG	80	0.0	44.000004	1
ACGCACG	20	7.856622E-4	44.000004	11
TAGCGAT	20	7.856622E-4	44.000004	40
CAACCCG	20	7.856622E-4	44.000004	23
CGACCGG	20	7.856622E-4	44.000004	2
CGATTCA	20	7.856622E-4	44.000004	10
CTAAGCG	20	7.856622E-4	44.000004	1
CACGACA	20	7.856622E-4	44.000004	16
CGTTCTA	20	7.856622E-4	44.000004	31
TAGCCGT	20	7.856622E-4	44.000004	44
ATCGTAC	40	8.312782E-9	44.000004	34
CTTATCG	40	8.312782E-9	44.000004	31
AGCGATA	20	7.856622E-4	44.000004	41
CGAATAT	40	8.312782E-9	44.000004	14
GCGATTC	20	7.856622E-4	44.000004	9
TACTCGT	20	7.856622E-4	44.000004	40
ACGTACC	20	7.856622E-4	44.000004	15
>>END_MODULE