FastQCFastQC Report
Thu 26 May 2016
SRR1546205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546205_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1796387
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236321.3155294488325733No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33190.18475974275030937No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA29040.16165781649499802No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC28590.15915278834683172No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA23770.13232115351536167No Hit
TTTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT23330.12987179265937684No Hit
CCACACCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATA22850.12719976263466615No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA22780.12681009158939582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22120.12313605030541859No Hit
ATGCAAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAG21640.12046402028070789No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19280.10732653932588021No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG18610.10359683074972151TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC18390.10237215032172912Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCAA950.044.00000432
GTATGCG1300.044.0000041
ATGCGGA950.044.0000043
AATACGG1750.044.0000042
AACCGTG207.857668E-444.022
CCTTATG254.4438446E-544.028
TAATACG1000.044.01
GTACACG302.5285153E-644.01
ACGTTGT207.857668E-444.030
ACGTTCA254.4438446E-544.035
GCGAAGT454.802132E-1044.043
CGCATCG302.5285153E-644.021
CAACCGT207.857668E-444.021
CGACATA207.857668E-444.028
TCGATAG551.8189894E-1244.01
ATCGTAG207.857668E-444.012
ATCCGAT207.857668E-444.025
TAACCGT207.857668E-444.036
TCTACGG800.044.02
GAACGTT254.4438446E-544.09
Produced by FastQC (version 0.11.5)