##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546205_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1796387
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82581314605372	31.0	31.0	34.0	30.0	34.0
2	32.179329955070926	33.0	31.0	34.0	30.0	34.0
3	32.12011387301288	33.0	31.0	34.0	30.0	34.0
4	35.882818679939234	37.0	35.0	37.0	35.0	37.0
5	35.85804116818926	37.0	35.0	37.0	35.0	37.0
6	35.92594969792144	37.0	35.0	37.0	35.0	37.0
7	36.14575311444583	37.0	35.0	37.0	35.0	37.0
8	36.03473193693787	37.0	35.0	37.0	35.0	37.0
9	37.80610636794855	39.0	38.0	39.0	35.0	39.0
10	37.443471256472016	39.0	37.0	39.0	35.0	39.0
11	37.29083042796458	39.0	37.0	39.0	34.0	39.0
12	36.75631865516729	39.0	35.0	39.0	33.0	39.0
13	36.52936087825174	39.0	35.0	39.0	33.0	39.0
14	37.704718415352595	40.0	35.0	41.0	33.0	41.0
15	37.8696171815984	40.0	35.0	41.0	33.0	41.0
16	37.980427380068996	40.0	35.0	41.0	33.0	41.0
17	37.898217366302475	40.0	35.0	41.0	33.0	41.0
18	37.73310817769222	39.0	36.0	41.0	33.0	41.0
19	37.49861638945283	38.0	36.0	41.0	33.0	41.0
20	37.23998113992141	38.0	35.0	41.0	33.0	41.0
21	37.13175668717264	38.0	35.0	41.0	33.0	41.0
22	37.03452763797556	38.0	35.0	41.0	33.0	41.0
23	36.983108873533375	38.0	35.0	41.0	33.0	41.0
24	36.908809181985845	38.0	35.0	41.0	33.0	41.0
25	36.89559710685949	38.0	35.0	41.0	33.0	41.0
26	36.829759957069385	38.0	35.0	41.0	33.0	41.0
27	36.75724328889042	38.0	35.0	41.0	32.0	41.0
28	36.68469656037368	38.0	35.0	41.0	32.0	41.0
29	36.61391504169202	38.0	35.0	41.0	32.0	41.0
30	36.397561327264114	38.0	35.0	41.0	31.0	41.0
31	36.118233431883	38.0	35.0	41.0	31.0	41.0
32	35.77629764633122	38.0	35.0	41.0	29.0	41.0
33	35.382276202176925	38.0	35.0	41.0	26.0	41.0
34	34.941489222533896	38.0	35.0	41.0	23.0	41.0
35	34.71274508221224	38.0	34.0	41.0	21.0	41.0
36	34.48300227066885	38.0	34.0	41.0	20.0	41.0
37	34.47458203605348	38.0	34.0	41.0	20.0	41.0
38	34.4408626871604	38.0	34.0	41.0	19.0	41.0
39	34.37136485623643	38.0	34.0	41.0	18.0	41.0
40	34.18924151644384	38.0	34.0	40.0	18.0	41.0
41	34.12253540022278	38.0	34.0	40.0	18.0	41.0
42	34.04989348063641	38.0	34.0	40.0	18.0	41.0
43	33.90902572775243	37.0	34.0	40.0	17.0	41.0
44	33.75702395975923	37.0	34.0	40.0	15.0	41.0
45	33.69604155452027	37.0	33.0	40.0	15.0	41.0
46	33.58177163384059	37.0	33.0	40.0	15.0	41.0
47	33.576883488914135	37.0	33.0	40.0	15.0	41.0
48	33.520808712153894	37.0	33.0	40.0	15.0	41.0
49	33.40585241376162	36.0	33.0	40.0	15.0	41.0
50	33.27293729023869	36.0	33.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	3.0
12	4.0
13	7.0
14	13.0
15	39.0
16	137.0
17	313.0
18	769.0
19	1458.0
20	2735.0
21	4319.0
22	6716.0
23	10636.0
24	16464.0
25	27487.0
26	42214.0
27	51400.0
28	48607.0
29	42120.0
30	41511.0
31	44251.0
32	52015.0
33	66840.0
34	120116.0
35	239654.0
36	96115.0
37	122798.0
38	206250.0
39	551388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.68830602759873	23.492654979133114	27.798241692909155	20.020797300358996
2	34.74028703169195	24.800502341644645	29.9167161641673	10.54249446249611
3	25.780079682162025	24.056787318100163	39.10705209957543	11.056080900162382
4	23.086172411624	26.477869189656793	38.60086941176929	11.835088986949916
5	19.55486206480007	30.451456172862528	38.36561943501039	11.628062327327019
6	16.427584924629272	40.339525948473245	34.38000831669345	8.852880810204038
7	78.77678918852118	3.844104861591628	15.695894036195988	1.6832119136912036
8	78.48475857373718	3.490283552486185	15.273100952077698	2.7518569216989435
9	74.88920817173582	5.097119941304407	17.267493029063335	2.746178857896433
10	37.10186056790658	29.752664654108496	23.279616251954618	9.865858526030303
11	32.81881910746404	19.457611305359034	34.11119096274911	13.612378624427809
12	34.60651852857987	19.693807626085025	33.83313283830266	11.866541007032449
13	17.770057342877678	35.96240676424401	31.23597532157603	15.031560571302286
14	11.022569190269135	38.0041160395839	32.70943287832744	18.263881891819526
15	10.523623250446592	26.587255418793386	49.341372432554905	13.54774889820512
16	11.514890722322082	20.549692243375176	46.509465944698995	21.425951089603743
17	12.376230734246016	23.593078774228495	35.06488301240211	28.965807479123374
18	14.20679397034158	27.962181868383595	42.27903007536795	15.551994085906879
19	18.8396486948525	27.34004421096345	33.43945374799528	20.380853346188765
20	21.778547718281192	22.306162313577197	38.65119264390135	17.264097324240264
21	15.628258276195497	29.69532734316158	36.27909798946441	18.397316391178514
22	19.72770900702354	23.441997743247974	31.777395405333035	25.052897844395446
23	14.001827000529396	28.86282298858765	32.08028114209243	25.05506886879052
24	12.290837108039637	23.986646529951507	50.07879705208288	13.643719309925977
25	13.467754999340343	30.617511705439863	39.4190116049604	16.495721690259394
26	13.465973645990537	29.40841811925827	34.41747240433158	22.708135830419614
27	12.528035440024896	34.4504831085952	32.73381515230293	20.28766629907698
28	10.968516249560924	30.10553961924686	40.80802187947252	18.1179222517197
29	11.358576965876505	23.10515495825788	38.57214508900365	26.96412298686196
30	12.78705535054529	33.56726585084394	36.00215321086158	17.64352558774919
31	19.069109273224534	35.35852797865939	30.395788880681057	15.176573867435023
32	19.243848903382176	25.578953755510366	33.59966421489356	21.577533126213893
33	18.87193572431776	33.42503591932028	28.69292641284979	19.010101943512172
34	13.872511880791835	28.14900130094462	33.78687331849986	24.191613499763694
35	19.06599190486237	25.645253500498498	34.91296697203888	20.375787622600253
36	22.99927576852872	32.39519101396303	28.138257513553594	16.46727570395466
37	14.49810091032723	33.1999173897384	35.95901105942094	16.34297064051343
38	14.559891604648664	29.324917180986056	38.05772364195465	18.05746757241062
39	14.987026737557107	32.21388264332797	33.98104083362883	18.81804978548609
40	18.099552045299816	28.11276189373448	33.48125988442357	20.306426176542136
41	13.61276829547308	28.13280211891981	35.36626573227261	22.888163853334497
42	15.856716843308263	26.34432335571344	31.056670973459504	26.74228882751879
43	16.46387999913159	27.390423110387687	33.31236531994498	22.833331570535748
44	14.847969841687789	27.948098043461684	32.36474100513976	24.83919110971077
45	13.92450513168933	39.171960162259026	26.269395180437176	20.63413952561447
46	17.88289494412952	35.40879554349925	30.0209810024232	16.687328509948024
47	16.379488384184477	29.50750589934129	34.18500579218175	19.927999924292482
48	15.317189447485424	27.087704375504835	36.55175638656926	21.04334979044048
49	17.19356686504634	28.603580408898527	34.080740953925854	20.12211177212928
50	16.46911272459665	30.49983104976823	33.5995528803092	19.431503345325922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3446.0
1	5820.0
2	8194.0
3	25312.0
4	42430.0
5	31595.5
6	20761.0
7	21746.0
8	22731.0
9	23909.5
10	25088.0
11	24579.0
12	24070.0
13	23193.0
14	22316.0
15	20023.5
16	17731.0
17	16130.0
18	14529.0
19	14645.5
20	14762.0
21	13987.5
22	13213.0
23	13887.5
24	14562.0
25	18503.0
26	22444.0
27	30843.5
28	39243.0
29	43207.5
30	47172.0
31	62993.5
32	78815.0
33	98515.0
34	118215.0
35	115519.5
36	112824.0
37	104860.0
38	96896.0
39	111055.0
40	125214.0
41	137228.5
42	149243.0
43	148704.0
44	148165.0
45	140267.5
46	132370.0
47	126109.5
48	119849.0
49	111761.0
50	103673.0
51	83378.0
52	63083.0
53	52756.0
54	42429.0
55	36973.5
56	31518.0
57	28945.0
58	26372.0
59	25107.0
60	23842.0
61	22047.5
62	20253.0
63	18218.5
64	16184.0
65	13543.0
66	10902.0
67	9526.5
68	8151.0
69	7383.0
70	6615.0
71	5328.0
72	4041.0
73	3172.0
74	2303.0
75	1808.0
76	1313.0
77	1045.0
78	777.0
79	557.5
80	338.0
81	267.5
82	197.0
83	129.0
84	61.0
85	42.0
86	23.0
87	18.0
88	13.0
89	12.0
90	11.0
91	7.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1796387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.983825607580656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21363052158871	17.19434813522354
2	9.023704407608314	3.967510880624361
3	3.6172592765492837	2.385635913391885
4	1.882882501023137	1.6557184216823178
5	1.133637620262213	1.2460845873018616
6	0.7553812957251089	0.9963702403469462
7	0.5346225591234597	0.8227134373954029
8	0.39904875442184307	0.7018094580905662
9	0.3229264713032012	0.6389243306280876
>10	2.7495135087151397	13.509868433679422
>50	0.7377024485020324	11.315116502023551
>100	0.5188834239079906	23.412493149722103
>500	0.08355118458949179	12.74531224523118
>1k	0.027001297458799173	8.084720513662317
>5k	0.0	0.0
>10k+	2.547292213094261E-4	1.323373750996508
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23632	1.3155294488325733	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3319	0.18475974275030937	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2904	0.16165781649499802	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2859	0.15915278834683172	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2377	0.13232115351536167	No Hit
TTTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2333	0.12987179265937684	No Hit
CCACACCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATA	2285	0.12719976263466615	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2278	0.12681009158939582	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2212	0.12313605030541859	No Hit
ATGCAAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAG	2164	0.12046402028070789	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1928	0.10732653932588021	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	1861	0.10359683074972151	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1839	0.10237215032172912	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11461895460165321	0.0
2	0.0	0.0	0.0	0.43915926801964167	0.0
3	0.0	0.0	0.0	0.618630618012711	0.0
4	0.0	0.0	0.0	0.9472903110521286	0.0
5	0.0	0.0	0.0	1.6127371217894586	0.0
6	0.0	0.0	0.0	2.2299203902054514	0.0
7	0.0	0.0	0.0	2.5959328362986374	0.0
8	0.0	0.0	0.0	3.4969636275479616	0.0
9	0.0	0.0	0.0	3.8147681986119917	0.0
10	0.0	0.0	0.0	4.408849540772673	0.0
11	0.0	0.0	0.0	5.052085101929595	0.0
12	0.0	0.0	0.0	5.582594396419035	0.0
13	0.0	0.0	0.0	5.780491620124171	0.0
14	0.0	0.0	0.0	5.849073724091746	0.0
15	0.0	0.0	0.0	5.966698712471199	0.0
16	0.0	0.0	0.0	6.256669637444492	0.0
17	0.0	0.0	0.0	6.592287742006595	0.0
18	0.0	0.0	0.0	7.111106905137924	0.0
19	0.0	0.0	0.0	7.332662728020187	0.0
20	5.566729218147314E-5	0.0	0.0	7.55761425572552	0.0
21	5.566729218147314E-5	0.0	0.0	7.886886288978934	0.0
22	5.566729218147314E-5	0.0	0.0	8.205525869425687	0.0
23	5.566729218147314E-5	0.0	0.0	8.628263286251793	0.0
24	5.566729218147314E-5	0.0	0.0	8.933988054912444	0.0
25	5.566729218147314E-5	0.0	0.0	9.19083694103776	0.0
26	5.566729218147314E-5	0.0	0.0	9.4530298872125	0.0
27	5.566729218147314E-5	0.0	0.0	9.6888922041854	0.0
28	5.566729218147314E-5	0.0	0.0	9.935665310425872	0.0
29	1.1133458436294629E-4	0.0	0.0	10.196967579925706	0.0
30	1.1133458436294629E-4	0.0	0.0	10.584968606430575	0.0
31	1.1133458436294629E-4	0.0	0.0	10.949700704803586	0.0
32	1.1133458436294629E-4	0.0	0.0	11.236999599752169	0.0
33	1.1133458436294629E-4	0.0	0.0	11.524465496577296	0.0
34	1.1133458436294629E-4	0.0	0.0	11.794284861780897	0.0
35	1.6700187654441944E-4	0.0	0.0	12.238120182343783	0.0
36	1.6700187654441944E-4	0.0	0.0	12.535884528222482	0.0
37	1.6700187654441944E-4	0.0	0.0	12.83019750198593	0.0
38	1.6700187654441944E-4	0.0	0.0	13.105193925362409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGCAA	95	0.0	44.000004	32
GTATGCG	130	0.0	44.000004	1
ATGCGGA	95	0.0	44.000004	3
AATACGG	175	0.0	44.000004	2
AACCGTG	20	7.857668E-4	44.0	22
CCTTATG	25	4.4438446E-5	44.0	28
TAATACG	100	0.0	44.0	1
GTACACG	30	2.5285153E-6	44.0	1
ACGTTGT	20	7.857668E-4	44.0	30
ACGTTCA	25	4.4438446E-5	44.0	35
GCGAAGT	45	4.802132E-10	44.0	43
CGCATCG	30	2.5285153E-6	44.0	21
CAACCGT	20	7.857668E-4	44.0	21
CGACATA	20	7.857668E-4	44.0	28
TCGATAG	55	1.8189894E-12	44.0	1
ATCGTAG	20	7.857668E-4	44.0	12
ATCCGAT	20	7.857668E-4	44.0	25
TAACCGT	20	7.857668E-4	44.0	36
TCTACGG	80	0.0	44.0	2
GAACGTT	25	4.4438446E-5	44.0	9
>>END_MODULE