FastQCFastQC Report
Thu 26 May 2016
SRR1546204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546204_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1845084
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244771.3266062683324986No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA32940.17852845724097116No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC29430.1595049331087365No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA29270.15863776391752354No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA24170.13099674594760996No Hit
TTTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT24010.130129576756397No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA23850.12926240756518403No Hit
ATGCAAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAG22850.12384260012010292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22510.12199986558877535No Hit
CCACACCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATA22210.12037392335525103No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA20590.11159383529421966No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG19760.10709539511480236TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG1500.044.0000041
ACGTAAG650.044.0000041
AGGGCGT650.044.0000046
CGTTTAG650.044.00000430
TGCGTAG650.044.0000041
AACCGTG351.446715E-744.022
CTATGCG351.446715E-744.01
TCGCAAG254.4438922E-544.01
ACACGAT207.857722E-444.032
TCCGCGA207.857722E-444.019
ATTACGT254.4438922E-544.034
CCGTGTG254.4438922E-544.038
CAACCGT351.446715E-744.021
GCCGGAT207.857722E-444.019
TCCTACG408.3164196E-944.027
ATCGTTC207.857722E-444.020
GCCGATG600.044.09
AAGCACG207.857722E-444.01
CGCTAGC254.4438922E-544.021
TCATGCG254.4438922E-544.034
Produced by FastQC (version 0.11.5)