##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546204_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1845084
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763204818859197	31.0	31.0	34.0	30.0	34.0
2	32.14933358047655	33.0	31.0	34.0	30.0	34.0
3	32.20701875903753	33.0	31.0	34.0	30.0	34.0
4	35.91871914774612	37.0	35.0	37.0	35.0	37.0
5	35.8207442045999	37.0	35.0	37.0	35.0	37.0
6	35.9116354594154	37.0	35.0	37.0	35.0	37.0
7	36.12504904925738	37.0	35.0	37.0	35.0	37.0
8	36.01563885438278	37.0	35.0	37.0	35.0	37.0
9	37.777262173429506	39.0	38.0	39.0	35.0	39.0
10	37.43681371688227	39.0	37.0	39.0	34.0	39.0
11	37.29001227044406	39.0	37.0	39.0	34.0	39.0
12	36.74326534726874	39.0	35.0	39.0	33.0	39.0
13	36.47684712457536	39.0	35.0	39.0	33.0	39.0
14	37.6422189992434	40.0	35.0	41.0	33.0	41.0
15	37.83538581441279	40.0	35.0	41.0	33.0	41.0
16	37.93941847634037	40.0	35.0	41.0	33.0	41.0
17	37.863616507432724	40.0	35.0	41.0	33.0	41.0
18	37.70748323653557	39.0	36.0	41.0	33.0	41.0
19	37.48411075051325	38.0	36.0	41.0	33.0	41.0
20	37.22545748594644	38.0	35.0	41.0	33.0	41.0
21	37.111684888059294	38.0	35.0	41.0	33.0	41.0
22	37.02218001998825	38.0	35.0	41.0	33.0	41.0
23	36.953875812700126	38.0	35.0	41.0	33.0	41.0
24	36.888831077609474	38.0	35.0	41.0	33.0	41.0
25	36.86863633308836	38.0	35.0	41.0	33.0	41.0
26	36.81862126602366	38.0	35.0	41.0	33.0	41.0
27	36.73977715919709	38.0	35.0	41.0	32.0	41.0
28	36.65748334493172	38.0	35.0	41.0	32.0	41.0
29	36.58651096643838	38.0	35.0	41.0	32.0	41.0
30	36.37361388424592	38.0	35.0	41.0	31.0	41.0
31	36.109934290254536	38.0	35.0	41.0	31.0	41.0
32	35.771709580702016	38.0	35.0	41.0	29.0	41.0
33	35.36541317360077	38.0	35.0	41.0	26.0	41.0
34	34.993223614751415	38.0	35.0	41.0	23.0	41.0
35	34.73122145116428	38.0	34.0	41.0	21.0	41.0
36	34.48525866573012	38.0	34.0	41.0	20.0	41.0
37	34.41415675383885	38.0	34.0	40.0	20.0	41.0
38	34.402574083347965	38.0	34.0	41.0	19.0	41.0
39	34.34432741273568	38.0	34.0	41.0	18.0	41.0
40	34.17493024707818	38.0	34.0	40.0	18.0	41.0
41	34.124801364057134	38.0	34.0	40.0	18.0	41.0
42	34.03610621521839	38.0	34.0	40.0	18.0	41.0
43	33.931225353425646	37.0	34.0	40.0	17.0	41.0
44	33.77098983027331	37.0	34.0	40.0	15.0	41.0
45	33.707686479314766	37.0	34.0	40.0	15.0	41.0
46	33.599687602298864	37.0	33.0	40.0	15.0	41.0
47	33.574607985327496	37.0	33.0	40.0	15.0	41.0
48	33.53776305035435	37.0	33.0	40.0	15.0	41.0
49	33.417084533820685	36.0	33.0	40.0	15.0	41.0
50	33.25720238211377	36.0	33.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	6.0
13	9.0
14	16.0
15	38.0
16	105.0
17	310.0
18	752.0
19	1611.0
20	2681.0
21	4570.0
22	6962.0
23	10935.0
24	17005.0
25	27877.0
26	42956.0
27	51957.0
28	49870.0
29	43974.0
30	42988.0
31	46228.0
32	54354.0
33	70358.0
34	125977.0
35	242580.0
36	99854.0
37	127504.0
38	212747.0
39	560854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.92117648844172	23.606188119348495	27.72898144474723	19.743653947462555
2	34.78974398997553	24.691179371779278	29.928772890556743	10.590303747688452
3	25.74923418120801	24.123454541907037	39.07025371202612	11.057057564858836
4	23.168755460456	26.39971947076664	38.650977408074645	11.78054766070271
5	19.582794062492546	30.50343507395869	38.22823242735832	11.68553843619044
6	16.42104099325559	40.41360718536392	34.31318032132955	8.852171500050947
7	78.85196554736802	3.848280078305378	15.614465249278622	1.6852891250479654
8	78.49810631927869	3.5040139093938274	15.236813066505373	2.761066704822111
9	74.91198232709189	5.117924170390074	17.192116998467277	2.777976504050764
10	37.184648503807956	29.68932579763306	23.229294709617555	9.896730988941425
11	32.863056641323645	19.513149536823253	33.97904919234029	13.644744629512804
12	34.578750886138515	19.727123534755055	33.82843274344149	11.865692835664934
13	17.86525708314635	35.84313776500149	31.248225013061738	15.043380138790429
14	11.089847399901576	37.96244507025154	32.690652566495615	18.257054963351262
15	10.568190933312522	26.543019179614586	49.3617092771928	13.527080609880093
16	11.651122658914174	20.531314563456192	46.44742461589825	21.37013816173139
17	12.395912598017219	23.527167326799216	35.08479830728574	28.99212176789783
18	14.298481803538484	27.870492617138297	42.274118685111354	15.556906894211863
19	18.866945895146237	27.31675089047436	33.43555090174756	20.380752312631838
20	21.78822210804495	22.432420420967283	38.53477673645211	17.244580734535663
21	15.702970704856797	29.575347247063004	36.30403819013118	18.417643857949013
22	19.73807154579412	23.479202030910244	31.730371083376152	25.052355339919487
23	13.98407877364933	28.774733291275627	32.09263101300537	25.14855692206967
24	12.355751824849166	23.99088605180035	49.9030396448075	13.750322478542982
25	13.488762571243369	30.49330003403639	39.3661210004531	16.651816394267144
26	13.46551159730397	29.356766412802887	34.488836280624625	22.688885709268522
27	12.517153690563681	34.39848809051512	32.84105222309662	20.24330599582458
28	10.962590321091072	30.09700371365206	40.733050636177	18.20735532907987
29	11.441972289608495	23.130871006414885	38.44578349820388	26.981373205772748
30	12.841474968077335	33.42731279443104	36.0217204203169	17.70949181717472
31	19.0048257965491	35.237149094566966	30.48739244392125	15.27063266496268
32	19.231048559306785	25.61742446414364	33.61890298761466	21.532623988934922
33	18.89317776318043	33.37848032935086	28.7292611068114	18.999080800657314
34	13.895952704592313	28.142133366285766	33.708980187351905	24.252933741770022
35	18.99073429719189	25.661975281342205	34.88301887610537	20.464271545360535
36	22.910989418367944	32.377712884616635	28.116118290549373	16.595179406466045
37	14.570122552685948	33.176971888542745	35.86871925614227	16.38418630262904
38	14.566328687474392	29.288856225516017	37.98195637705384	18.162858709955753
39	14.977366884109342	32.18693566254978	33.98333084022191	18.85236661311897
40	18.148171031779583	28.00663818015873	33.46584762536556	20.379343162696113
41	13.636235531824026	28.119857957686477	35.3060890452684	22.937817465221098
42	15.871906102919976	26.28834242777023	31.0551172737935	26.7846341955163
43	16.513177719821968	27.341085825902777	33.32493263179346	22.820803822481793
44	14.8442022151837	27.914555651666806	32.36443435637619	24.87680777677331
45	13.998441263378794	39.01676021254317	26.326281079885796	20.658517444192242
46	17.88449739957639	35.288908255667494	30.037873614426225	16.788720730329892
47	16.426406602626223	29.52954987415207	33.98988880723046	20.054154715991253
48	15.32082008190413	27.080880870464437	36.48424678768013	21.114052259951308
49	17.26094855301981	28.55490590130314	33.973141602225155	20.211003943451896
50	16.52461351353109	30.358617819026122	33.561182038324546	19.555586629118242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3510.0
1	6055.5
2	8601.0
3	26303.0
4	44005.0
5	32438.5
6	20872.0
7	21783.5
8	22695.0
9	23995.0
10	25295.0
11	24834.5
12	24374.0
13	23520.0
14	22666.0
15	20471.5
16	18277.0
17	16562.0
18	14847.0
19	14937.0
20	15027.0
21	14162.5
22	13298.0
23	14174.0
24	15050.0
25	19075.5
26	23101.0
27	31820.5
28	40540.0
29	44206.5
30	47873.0
31	63950.5
32	80028.0
33	100205.0
34	120382.0
35	117749.0
36	115116.0
37	107415.0
38	99714.0
39	113984.0
40	128254.0
41	140203.5
42	152153.0
43	151917.0
44	151681.0
45	144366.5
46	137052.0
47	131064.5
48	125077.0
49	116557.5
50	108038.0
51	87375.5
52	66713.0
53	55608.5
54	44504.0
55	38753.0
56	33002.0
57	29892.0
58	26782.0
59	25614.0
60	24446.0
61	22638.5
62	20831.0
63	18589.5
64	16348.0
65	13672.0
66	10996.0
67	9666.5
68	8337.0
69	7414.0
70	6491.0
71	5339.0
72	4187.0
73	3204.0
74	2221.0
75	1774.5
76	1328.0
77	996.0
78	664.0
79	509.0
80	354.0
81	285.5
82	217.0
83	144.5
84	72.0
85	53.5
86	35.0
87	24.5
88	14.0
89	12.5
90	11.0
91	6.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1845084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.017797200151797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05675723845678	17.186378509778223
2	9.07705722692758	3.997136103933275
3	3.6404679109771556	2.404652525326658
4	1.8711320245724221	1.6479282180698016
5	1.1475287479516774	1.2633027626872069
6	0.7931462823581193	1.0478000397009244
7	0.5523825366888462	0.8513572668804299
8	0.4368012005848226	0.7693920201007559
9	0.31915234908036827	0.6324328528203241
>10	2.752412772167933	13.46694292525937
>50	0.7254085152278078	11.12692874261294
>100	0.5169732194575661	23.286716857813012
>500	0.08227953441222462	12.481257231956025
>1k	0.028252611213836164	8.502145076051363
>5k	0.0	0.0
>10k+	2.478299229283874E-4	1.3356288670096603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24477	1.3266062683324986	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3294	0.17852845724097116	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2943	0.1595049331087365	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2927	0.15863776391752354	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2417	0.13099674594760996	No Hit
TTTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2401	0.130129576756397	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2385	0.12926240756518403	No Hit
ATGCAAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAG	2285	0.12384260012010292	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2251	0.12199986558877535	No Hit
CCACACCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATA	2221	0.12037392335525103	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2059	0.11159383529421966	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	1976	0.10709539511480236	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.419807445081091E-5	0.0	0.0	0.11489991783571914	0.0
2	5.419807445081091E-5	0.0	0.0	0.4516867524730581	0.0
3	5.419807445081091E-5	0.0	0.0	0.6262045522046693	0.0
4	5.419807445081091E-5	0.0	0.0	0.9433716838908147	0.0
5	5.419807445081091E-5	0.0	0.0	1.6236117163229424	0.0
6	5.419807445081091E-5	0.0	0.0	2.2401690112753676	0.0
7	5.419807445081091E-5	0.0	0.0	2.63066613769346	0.0
8	5.419807445081091E-5	0.0	0.0	3.5452044459764434	0.0
9	5.419807445081091E-5	0.0	0.0	3.8805279326036106	0.0
10	5.419807445081091E-5	0.0	0.0	4.4935081546422815	0.0
11	5.419807445081091E-5	0.0	0.0	5.168870360373837	0.0
12	5.419807445081091E-5	0.0	0.0	5.703317572533283	0.0
13	5.419807445081091E-5	0.0	0.0	5.913877091774683	0.0
14	5.419807445081091E-5	0.0	0.0	5.9855269461986556	0.0
15	5.419807445081091E-5	0.0	0.0	6.107743604085234	0.0
16	5.419807445081091E-5	0.0	0.0	6.41493829007243	0.0
17	5.419807445081091E-5	0.0	0.0	6.769014310459578	0.0
18	5.419807445081091E-5	0.0	0.0	7.308014160872893	0.0
19	5.419807445081091E-5	0.0	0.0	7.545239132744092	0.0
20	5.419807445081091E-5	0.0	0.0	7.78506561218893	0.0
21	5.419807445081091E-5	0.0	0.0	8.135401965438971	0.0
22	5.419807445081091E-5	0.0	0.0	8.467094181077934	0.0
23	5.419807445081091E-5	0.0	0.0	8.908266507107536	0.0
24	5.419807445081091E-5	0.0	0.0	9.233725944184656	0.0
25	5.419807445081091E-5	0.0	0.0	9.508564379724717	0.0
26	5.419807445081091E-5	0.0	0.0	9.781993665329058	0.0
27	5.419807445081091E-5	0.0	0.0	10.02669797147447	0.0
28	5.419807445081091E-5	0.0	0.0	10.298067730249679	0.0
29	5.419807445081091E-5	0.0	0.0	10.568190933312522	0.0
30	5.419807445081091E-5	0.0	0.0	10.973755124427939	0.0
31	5.419807445081091E-5	0.0	0.0	11.354550795519337	0.0
32	5.419807445081091E-5	0.0	0.0	11.658764587411738	0.0
33	5.419807445081091E-5	0.0	0.0	11.968072998302516	0.0
34	5.419807445081091E-5	0.0	0.0	12.250499164265692	0.0
35	5.419807445081091E-5	0.0	0.0	12.711995768214347	0.0
36	5.419807445081091E-5	0.0	0.0	13.020437009913913	0.0
37	5.419807445081091E-5	0.0	0.0	13.328065280496714	0.0
38	5.419807445081091E-5	0.0	0.0	13.610112059938734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	150	0.0	44.000004	1
ACGTAAG	65	0.0	44.000004	1
AGGGCGT	65	0.0	44.000004	6
CGTTTAG	65	0.0	44.000004	30
TGCGTAG	65	0.0	44.000004	1
AACCGTG	35	1.446715E-7	44.0	22
CTATGCG	35	1.446715E-7	44.0	1
TCGCAAG	25	4.4438922E-5	44.0	1
ACACGAT	20	7.857722E-4	44.0	32
TCCGCGA	20	7.857722E-4	44.0	19
ATTACGT	25	4.4438922E-5	44.0	34
CCGTGTG	25	4.4438922E-5	44.0	38
CAACCGT	35	1.446715E-7	44.0	21
GCCGGAT	20	7.857722E-4	44.0	19
TCCTACG	40	8.3164196E-9	44.0	27
ATCGTTC	20	7.857722E-4	44.0	20
GCCGATG	60	0.0	44.0	9
AAGCACG	20	7.857722E-4	44.0	1
CGCTAGC	25	4.4438922E-5	44.0	21
TCATGCG	25	4.4438922E-5	44.0	34
>>END_MODULE