FastQCFastQC Report
Thu 26 May 2016
SRR1546203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546203_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2676550
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392781.4674861295324204No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.10393977321551998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27550.10293101193700846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA454.802132E-1044.040
GCGAATC207.85836E-444.024
ACGGGTA2800.044.05
CGAATCA254.44443E-544.037
ACCGTTG254.44443E-544.01
GCTAACG351.4470425E-744.01
CGATAGA502.7284841E-1144.010
ATACCCG207.85836E-444.023
CATACGA5300.043.1698118
TAACGCG1600.042.6251
CGTTTTT262300.042.448341
ACATACG5250.042.32380717
CCCTAGC44500.041.13258721
AGTCGTG750.041.06666615
GCCCTAC43700.040.97940426
CTACGGG4900.040.8571433
TTACGTG700.040.8571431
ACGTAAC700.040.85714314
GTGACGT700.040.85714311
ATACGAA5450.040.7706419
Produced by FastQC (version 0.11.5)