##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546203_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2676550 Sequences flagged as poor quality 0 Sequence length 50 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85425977470998 31.0 31.0 34.0 30.0 34.0 2 32.22322766247595 33.0 31.0 34.0 30.0 34.0 3 32.116366964936205 33.0 31.0 34.0 30.0 34.0 4 35.88056789523827 37.0 35.0 37.0 35.0 37.0 5 35.879247165194 37.0 35.0 37.0 35.0 37.0 6 35.935774037473614 37.0 35.0 37.0 35.0 37.0 7 36.171930657002484 37.0 35.0 37.0 35.0 37.0 8 36.07000317572995 37.0 35.0 37.0 35.0 37.0 9 37.83876109170387 39.0 38.0 39.0 35.0 39.0 10 37.43515458332555 39.0 37.0 39.0 34.0 39.0 11 37.277102239823655 39.0 37.0 39.0 34.0 39.0 12 36.85748407464833 39.0 35.0 39.0 33.0 39.0 13 36.71680297397769 39.0 35.0 39.0 33.0 39.0 14 37.784114251555174 40.0 36.0 41.0 33.0 41.0 15 37.95930619640956 40.0 36.0 41.0 33.0 41.0 16 38.0722601856868 40.0 36.0 41.0 33.0 41.0 17 37.96592479124246 40.0 36.0 41.0 33.0 41.0 18 37.792705161495206 39.0 36.0 41.0 33.0 41.0 19 37.560064261829595 39.0 36.0 41.0 33.0 41.0 20 37.28162933627244 39.0 35.0 41.0 33.0 41.0 21 37.20342642580935 39.0 35.0 41.0 33.0 41.0 22 37.12850497842372 39.0 35.0 41.0 33.0 41.0 23 37.09592423081953 38.0 35.0 41.0 33.0 41.0 24 37.031233117259156 38.0 35.0 41.0 33.0 41.0 25 37.000614597149315 38.0 35.0 41.0 33.0 41.0 26 36.92619043171246 38.0 35.0 41.0 33.0 41.0 27 36.86085669985616 38.0 35.0 41.0 33.0 41.0 28 36.759704096691635 38.0 35.0 41.0 32.0 41.0 29 36.622086641385366 38.0 35.0 41.0 32.0 41.0 30 36.39281911415815 38.0 35.0 41.0 31.0 41.0 31 36.06209448730642 38.0 35.0 41.0 30.0 41.0 32 35.72478862715062 38.0 35.0 41.0 29.0 41.0 33 35.342958659468344 38.0 35.0 41.0 25.0 41.0 34 34.87892286712372 38.0 34.0 41.0 23.0 41.0 35 34.604810670452636 38.0 34.0 41.0 21.0 41.0 36 34.41076983430162 38.0 34.0 41.0 19.0 41.0 37 34.37632138387103 38.0 34.0 41.0 18.0 41.0 38 34.27674954699146 38.0 34.0 41.0 18.0 41.0 39 34.18135958603426 38.0 34.0 40.0 18.0 41.0 40 34.01318787244774 38.0 34.0 40.0 18.0 41.0 41 33.864592105508954 37.0 34.0 40.0 16.0 41.0 42 33.79696699108928 37.0 33.0 40.0 15.0 41.0 43 33.69153574564271 37.0 33.0 40.0 15.0 41.0 44 33.57564439296856 37.0 33.0 40.0 15.0 41.0 45 33.527446526311856 37.0 33.0 40.0 15.0 41.0 46 33.44264482262614 37.0 33.0 40.0 15.0 41.0 47 33.39689861949151 37.0 33.0 40.0 15.0 41.0 48 33.29523117445966 36.0 33.0 40.0 15.0 41.0 49 33.19946460929181 36.0 33.0 40.0 15.0 41.0 50 33.054438362817805 36.0 32.0 40.0 14.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 3.0 11 4.0 12 9.0 13 13.0 14 26.0 15 60.0 16 165.0 17 457.0 18 1024.0 19 2137.0 20 3936.0 21 6499.0 22 10753.0 23 16480.0 24 25684.0 25 41820.0 26 63104.0 27 75772.0 28 73657.0 29 65446.0 30 64117.0 31 68912.0 32 80187.0 33 101782.0 34 180683.0 35 292247.0 36 160183.0 37 211035.0 38 353340.0 39 777007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.176850796734605 22.3271375464684 27.959350656628867 20.536661000168127 2 38.926864807307915 22.84911546580486 27.33163961069287 10.892380116194355 3 25.959836356503708 23.827090844557357 38.736134202611574 11.476938596327361 4 23.36593749416226 25.303095402663878 38.50837832284097 12.822588780332891 5 20.3356559750425 29.750499710448153 37.33201322598121 12.581831088528142 6 16.828491901888626 39.07395714632643 34.86073490127216 9.236816050512788 7 79.13246530048009 3.2461937942500607 15.949972912891596 1.671367992378248 8 79.40243970783284 2.677775494573238 15.509629934056901 2.410154863537016 9 76.30472062916814 3.341839308064486 17.5299919672713 2.8234480954960675 10 37.88190020735648 25.76167080756945 24.333451644841304 12.022977340232762 11 30.871233490874445 22.525489903046832 32.559974594160394 14.043302011918326 12 29.08303599783303 20.36588145186901 35.84128822551419 14.70979432478377 13 19.896994265005326 28.443331901141395 36.5017653322374 15.157908501615886 14 14.546412359193738 31.070333078029556 38.64041396573948 15.74284059703723 15 13.601875548747454 23.3955278249986 47.77388802749808 15.22870859875586 16 16.960004483383457 20.034447329584726 44.18452859090994 18.821019596121875 17 16.276961013244662 21.05213801348751 37.79297976873214 24.87792120453569 18 19.13967607554501 23.076983430161963 40.91894416319516 16.86439633109787 19 20.536810446283464 25.6962134090527 35.66908893911938 18.09788720554445 20 21.200911621303543 24.597186676878817 36.80958696829874 17.392314733518894 21 18.190954773869347 25.78476023238871 38.845304589863815 17.17898040387813 22 18.24374661411145 22.510246399282657 36.11335487848163 23.13265210812426 23 15.14901645775345 26.40611981842297 35.93095589471521 22.51390782910837 24 15.633109786852478 24.364162821542656 43.749565672227305 16.253161719377555 25 16.900637014066618 26.455063421195195 38.63107358353104 18.01322598120715 26 15.820515215482617 27.035400048569986 35.88384300685584 21.260241729091554 27 15.305337094393904 28.310025966262543 36.55825596383404 19.826380975509515 28 14.244381760101623 26.068334236236947 39.916795875287214 19.770488128374215 29 15.256767106910015 24.132483981244512 38.870075283480595 21.740673628364874 30 17.89916123367768 26.23253815546132 38.81365937494162 17.054641235919373 31 20.645569856718538 27.534213820029517 34.16693131082924 17.65328501242271 32 21.668864769946385 24.127178644150117 34.49246231155779 19.711494274345707 33 21.351889559320767 26.84194205226878 32.4147876931124 19.391380695298054 34 18.205152154826177 24.824157964543907 34.728773981431324 22.241915899198595 35 18.97670508677215 26.00511852945023 34.80626179223254 20.211914591545085 36 23.422241318114736 27.64816648297248 31.823504137789314 17.10608806112346 37 17.52580747604192 29.992901309521585 34.58276512674899 17.898526087687507 38 18.84691113560367 27.663447348265493 33.7288673852534 19.760774130877433 39 19.200313836842202 28.44796473071678 32.202350040163644 20.14937139227737 40 21.16063589322075 25.25336720778614 33.843567278773044 19.742429620220058 41 17.728269600792064 26.355644392968557 33.80781229567914 22.108273710560237 42 19.2438026564047 27.301488856924028 31.631092264295457 21.82361622237582 43 18.57760923576993 25.521361454110703 34.126655582746444 21.774373727372925 44 18.065420036987913 26.700864919392505 32.98884758364312 22.24486745997646 45 17.677794175337656 30.72070389120323 30.69944518129682 20.9020567521623 46 20.766172871793913 27.88238590723132 32.96971848088024 18.381722740094524 47 18.08260634025144 28.00754702882442 34.18960975883133 19.720236872092805 48 17.835497188544956 27.090881918888122 34.69638153593245 20.377239356634476 49 19.96402084773309 25.97029758457716 34.28368608843474 19.78199547925501 50 18.676542564121725 27.665165978591844 33.58943415964581 20.06885729764062 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4781.0 1 9377.5 2 13974.0 3 43216.5 4 72459.0 5 52268.0 6 32077.0 7 32473.5 8 32870.0 9 34169.5 10 35469.0 11 34723.0 12 33977.0 13 32403.0 14 30829.0 15 28109.0 16 25389.0 17 24211.5 18 23034.0 19 22541.0 20 22048.0 21 21111.5 22 20175.0 23 23890.0 24 27605.0 25 30883.5 26 34162.0 27 39482.0 28 44802.0 29 54584.0 30 64366.0 31 74470.0 32 84574.0 33 103993.0 34 123412.0 35 130541.5 36 137671.0 37 137492.5 38 137314.0 39 144456.5 40 151599.0 41 163519.0 42 175439.0 43 175723.0 44 176007.0 45 180145.5 46 184284.0 47 184861.0 48 185438.0 49 183734.5 50 182031.0 51 158415.0 52 134799.0 53 116064.5 54 97330.0 55 87595.0 56 77860.0 57 73036.5 58 68213.0 59 63322.0 60 58431.0 61 53503.0 62 48575.0 63 43897.5 64 39220.0 65 33793.5 66 28367.0 67 25138.5 68 21910.0 69 19703.0 70 17496.0 71 14736.0 72 11976.0 73 9846.5 74 7717.0 75 5806.0 76 3895.0 77 3190.0 78 2485.0 79 1888.0 80 1291.0 81 955.5 82 620.0 83 440.5 84 261.0 85 184.0 86 107.0 87 98.0 88 89.0 89 65.0 90 41.0 91 57.5 92 74.0 93 38.0 94 2.0 95 2.0 96 2.0 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2676550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.00406331593754 #Duplication Level Percentage of deduplicated Percentage of total 1 77.66072266101793 18.641729039165398 2 8.843684446325376 4.245687227915328 3 3.5037562544787795 2.523131609283624 4 1.883432763926859 1.8084015726644629 5 1.1589664549518626 1.3909952082856092 6 0.7800470631356162 1.1234579455751077 7 0.5875971648710796 0.9873303684881549 8 0.45186025761394333 0.8677185786976753 9 0.3745871447164271 0.8092452185198438 >10 3.479621879379597 19.03789999067462 >50 0.7900998218441424 12.926811206316055 >100 0.41012844927163655 19.82162658708614 >500 0.06293919132055177 10.510056259176093 >1k 0.012399491556916683 3.826082295690472 >5k 0.0 0.0 >10k+ 1.5695558932805927E-4 1.4798268924613784 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39278 1.4674861295324204 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.10393977321551998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2755 0.10293101193700846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0487941566568904 0.0 2 0.0 0.0 0.0 0.2530869963198894 0.0 3 0.0 0.0 0.0 0.409370271431507 0.0 4 0.0 0.0 0.0 0.682894024023463 0.0 5 0.0 0.0 0.0 1.3109413237189667 0.0 6 0.0 0.0 0.0 1.9440697913358616 0.0 7 0.0 0.0 0.0 2.3393174048682073 0.0 8 0.0 0.0 0.0 3.2381237040219686 0.0 9 0.0 0.0 0.0 3.665464870822514 0.0 10 0.0 0.0 0.0 4.425659898003026 0.0 11 0.0 0.0 0.0 5.23416338196559 0.0 12 0.0 0.0 0.0 5.886570398460705 0.0 13 0.0 0.0 0.0 6.135211372849377 0.0 14 0.0 0.0 0.0 6.229736040798789 0.0 15 0.0 0.0 0.0 6.361435429937793 0.0 16 0.0 0.0 0.0 6.6663428667501075 0.0 17 0.0 0.0 0.0 7.011264500943379 0.0 18 0.0 0.0 0.0 7.491957930918534 0.0 19 0.0 0.0 0.0 7.734210083876632 0.0 20 0.0 0.0 0.0 8.007509667295587 0.0 21 0.0 0.0 0.0 8.374698772673778 0.0 22 3.7361528833759874E-5 0.0 0.0 8.749509629934057 0.0 23 3.7361528833759874E-5 0.0 0.0 9.168519175804674 0.0 24 3.7361528833759874E-5 0.0 0.0 9.480525303095403 0.0 25 3.7361528833759874E-5 0.0 0.0 9.732117838261942 0.0 26 3.7361528833759874E-5 0.0 0.0 9.996562739347294 0.0 27 3.7361528833759874E-5 0.0 0.0 10.30337561413013 0.0 28 3.7361528833759874E-5 0.0 0.0 10.600698660589192 0.0 29 3.7361528833759874E-5 0.0 0.0 10.96545926659319 0.0 30 3.7361528833759874E-5 0.0 0.0 11.357045450299827 0.0 31 3.7361528833759874E-5 0.0 0.0 11.695503540004857 0.0 32 3.7361528833759874E-5 0.0 0.0 11.983523565784312 0.0 33 3.7361528833759874E-5 0.0 0.0 12.299228484429582 0.0 34 3.7361528833759874E-5 0.0 0.0 12.627262707589994 0.0 35 3.7361528833759874E-5 0.0 0.0 13.042760269750238 0.0 36 7.472305766751975E-5 0.0 0.0 13.339448170219125 0.0 37 7.472305766751975E-5 0.0 0.0 13.66015953372812 0.0 38 7.472305766751975E-5 0.0 0.0 14.032056191739366 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACA 45 4.802132E-10 44.0 40 GCGAATC 20 7.85836E-4 44.0 24 ACGGGTA 280 0.0 44.0 5 CGAATCA 25 4.44443E-5 44.0 37 ACCGTTG 25 4.44443E-5 44.0 1 GCTAACG 35 1.4470425E-7 44.0 1 CGATAGA 50 2.7284841E-11 44.0 10 ATACCCG 20 7.85836E-4 44.0 23 CATACGA 530 0.0 43.16981 18 TAACGCG 160 0.0 42.625 1 CGTTTTT 26230 0.0 42.44834 1 ACATACG 525 0.0 42.323807 17 CCCTAGC 4450 0.0 41.132587 21 AGTCGTG 75 0.0 41.066666 15 GCCCTAC 4370 0.0 40.979404 26 CTACGGG 490 0.0 40.857143 3 TTACGTG 70 0.0 40.857143 1 ACGTAAC 70 0.0 40.857143 14 GTGACGT 70 0.0 40.857143 11 ATACGAA 545 0.0 40.77064 19 >>END_MODULE