FastQCFastQC Report
Thu 26 May 2016
SRR1546202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546202_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2750853
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404901.471907077550127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29250.10633065452788644No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.10404045581497812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA502.7284841E-1144.040
CGGCATA750.044.033
CATTACG700.044.021
TACGCCC302.5290083E-644.017
TATTACG302.5290083E-644.01
CAATACG302.5290083E-644.014
CGCCGTT1650.042.66666826
TACGCGG2750.042.42
CGTTTTT275500.042.2990951
CATACGA5900.042.13559318
GCCCTAC41350.041.39298626
CTACGGG5650.041.2743383
CCCTAGC42600.041.2112721
ACTACGG2250.041.0666662
TACTACG1450.040.965521
TCCTAGC43750.040.83215
ATACGAA6050.040.72727219
ACATACG6050.040.72727217
GTACGAG2000.040.71
ATCCTAG43950.040.5961314
Produced by FastQC (version 0.11.5)