##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546202_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2750853
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795471804563892	31.0	31.0	34.0	30.0	34.0
2	32.19598211900091	33.0	31.0	34.0	30.0	34.0
3	32.20978474676764	33.0	31.0	34.0	30.0	34.0
4	35.922033638293286	37.0	35.0	37.0	35.0	37.0
5	35.84160949349165	37.0	35.0	37.0	35.0	37.0
6	35.92151779829747	37.0	35.0	37.0	35.0	37.0
7	36.146871170506024	37.0	35.0	37.0	35.0	37.0
8	36.04955226615163	37.0	35.0	37.0	35.0	37.0
9	37.80979063585004	39.0	38.0	39.0	35.0	39.0
10	37.428238440949045	39.0	37.0	39.0	34.0	39.0
11	37.264415801207846	39.0	37.0	39.0	34.0	39.0
12	36.83715305761522	39.0	35.0	39.0	33.0	39.0
13	36.663879531185415	39.0	35.0	39.0	33.0	39.0
14	37.70328367237362	40.0	36.0	41.0	33.0	41.0
15	37.915969701034555	40.0	36.0	41.0	33.0	41.0
16	38.025469917876386	40.0	36.0	41.0	33.0	41.0
17	37.928246983753766	40.0	36.0	41.0	33.0	41.0
18	37.766265954596626	39.0	36.0	41.0	33.0	41.0
19	37.54517707780096	39.0	36.0	41.0	33.0	41.0
20	37.26509159158996	39.0	35.0	41.0	33.0	41.0
21	37.180368780156556	39.0	35.0	41.0	33.0	41.0
22	37.11540274961985	39.0	35.0	41.0	33.0	41.0
23	37.07420643705789	38.0	35.0	41.0	33.0	41.0
24	37.013773545878315	38.0	35.0	41.0	33.0	41.0
25	36.973412974084766	38.0	35.0	41.0	33.0	41.0
26	36.91844566030973	38.0	35.0	41.0	33.0	41.0
27	36.84165529746591	38.0	35.0	41.0	32.0	41.0
28	36.73531664541871	38.0	35.0	41.0	32.0	41.0
29	36.60252547119021	38.0	35.0	41.0	32.0	41.0
30	36.38222216890543	38.0	35.0	41.0	31.0	41.0
31	36.05841315402895	38.0	35.0	41.0	30.0	41.0
32	35.732712725834496	38.0	35.0	41.0	29.0	41.0
33	35.33529708784875	38.0	35.0	41.0	25.0	41.0
34	34.93809047593601	38.0	35.0	41.0	23.0	41.0
35	34.63570318006815	38.0	34.0	41.0	21.0	41.0
36	34.42618453257953	38.0	34.0	41.0	20.0	41.0
37	34.331682572642016	38.0	34.0	40.0	18.0	41.0
38	34.242477878679814	38.0	34.0	40.0	18.0	41.0
39	34.15826763552978	38.0	34.0	40.0	18.0	41.0
40	34.004418629421494	38.0	34.0	40.0	18.0	41.0
41	33.87795567411272	37.0	34.0	40.0	16.0	41.0
42	33.7937952336966	37.0	33.0	40.0	15.0	41.0
43	33.718020192282175	37.0	33.0	40.0	15.0	41.0
44	33.59612854630909	37.0	33.0	40.0	15.0	41.0
45	33.54645413622611	37.0	33.0	40.0	15.0	41.0
46	33.47261849324555	37.0	33.0	40.0	15.0	41.0
47	33.40820974439565	36.0	33.0	40.0	15.0	41.0
48	33.32237382368305	36.0	33.0	40.0	15.0	41.0
49	33.21807199439592	36.0	33.0	40.0	15.0	41.0
50	33.05243718948268	36.0	32.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	10.0
12	4.0
13	11.0
14	25.0
15	63.0
16	173.0
17	445.0
18	1087.0
19	2236.0
20	4054.0
21	6841.0
22	10797.0
23	16974.0
24	26166.0
25	42538.0
26	63817.0
27	77796.0
28	76430.0
29	67947.0
30	66536.0
31	70774.0
32	83240.0
33	106756.0
34	190095.0
35	296476.0
36	165624.0
37	217262.0
38	362840.0
39	793831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.24002118615571	22.42093634229092	28.021962642133186	20.317079829420184
2	39.0160070349088	22.87566802006505	27.22188353939669	10.886441405629455
3	25.909599676900218	23.89040781168605	38.693525244715005	11.50646726669873
4	23.475591025765464	25.333160296097248	38.36871690344777	12.822531774689525
5	20.355031693805522	29.787087859656623	37.24870067575403	12.609179770783827
6	16.948451989255698	39.0099725430621	34.76834276495327	9.273232702728935
7	79.14043389450472	3.2478289461487035	15.925169392911945	1.6865677664346297
8	79.41027746666217	2.7009803868109272	15.434921458907475	2.45382068761944
9	76.30160535659303	3.343726473206674	17.497408985503768	2.8572591846965287
10	38.03969168835994	25.633794317617113	24.23924506325856	12.087268930764385
11	31.02321352685876	22.436640562036576	32.46956489496167	14.070581016142993
12	29.13423581703566	20.346634298524858	35.766978460862866	14.752151423576615
13	20.017718140518596	28.404898407875667	36.44647678374671	15.130906667859023
14	14.620810345009346	30.964649874057248	38.69796023269873	15.716579548234675
15	13.664888672713518	23.272926615853336	47.830109424240405	15.232075287192737
16	17.054164653654702	20.029859828933063	44.1199511569684	18.796024360443834
17	16.36852278184258	21.004648376339993	37.70590431404368	24.92092452777375
18	19.25144673306789	22.94608254239685	40.899241071769374	16.903229652765887
19	20.656283705454275	25.640374094871664	35.657012570282745	18.04632962939132
20	21.23152345836001	24.683616318283818	36.77681068381335	17.308049539542825
21	18.11136400236581	25.86634036787862	38.84064324774897	17.1816523820066
22	18.192029890364918	22.49135086462272	36.213058276832676	23.103560968179686
23	15.179109897911665	26.320999340931706	35.972623764337825	22.527266996818803
24	15.64038500057982	24.373021749980825	43.54940085857005	16.437192390869303
25	16.914935112854085	26.43441870576145	38.57552548245944	18.075120698925023
26	15.843303877015602	26.978104609733784	35.88599608921306	21.29259542403756
27	15.25948496702659	28.198889580795484	36.685384497099626	19.8562409550783
28	14.235802494717092	25.984376482494703	39.879266540233154	19.900554482555048
29	15.258612510374055	24.176101013031232	38.81326992027564	21.752016556319077
30	17.99332788774973	26.164393371801403	38.74201929365182	17.100259446797047
31	20.64359673163197	27.44141544459119	34.27351443352298	17.641473390253857
32	21.764594473059812	24.080385247775872	34.438154274328724	19.71686600483559
33	21.4071417120435	26.759263399389212	32.382101115544884	19.451493773022406
34	18.187740311823276	24.777259998989404	34.799496737920926	22.23550295126639
35	18.961573010262637	26.04777499924569	34.678806900986714	20.311845089504963
36	23.502128248946782	27.54109361714348	31.765819547609414	17.190958586300322
37	17.542267798388355	30.009600658413955	34.51740242026746	17.930729122930234
38	18.886105509818226	27.548255032166384	33.7624365969392	19.803202861076183
39	19.254827502596466	28.39202967225075	32.15871586013502	20.194426965017758
40	21.233631895270303	25.132677027816463	33.917043186240775	19.716647890672455
41	17.770378860666128	26.226192384689405	33.813838834717814	22.189589919926654
42	19.254682093154376	27.22642758446198	31.728013092666163	21.790877229717474
43	18.56267855825084	25.441344921011773	34.13981045152176	21.85616606921562
44	18.110927774039542	26.588152838410483	33.006089383911096	22.29483000363887
45	17.70509002116798	30.529403061523098	30.70734786628002	21.0581590510289
46	20.762868826505816	27.725581846794427	32.99867350236454	18.512875824335215
47	18.028407915653798	27.9331901777376	34.21498713308199	19.82341477352661
48	17.867403310900293	27.005805108451813	34.73773407739345	20.389057503254445
49	20.015536998887253	25.874010715948835	34.27983974425387	19.830612540910035
50	18.666500899902687	27.56508617508824	33.60604874197203	20.16236418303704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5144.0
1	9731.5
2	14319.0
3	44412.5
4	74506.0
5	53539.0
6	32572.0
7	33027.5
8	33483.0
9	34643.5
10	35804.0
11	35147.5
12	34491.0
13	33091.0
14	31691.0
15	28627.0
16	25563.0
17	24644.0
18	23725.0
19	23186.5
20	22648.0
21	21503.5
22	20359.0
23	24299.0
24	28239.0
25	31504.0
26	34769.0
27	40225.0
28	45681.0
29	55784.5
30	65888.0
31	75852.0
32	85816.0
33	105626.5
34	125437.0
35	132425.0
36	139413.0
37	139449.5
38	139486.0
39	147106.0
40	154726.0
41	167138.5
42	179551.0
43	179811.5
44	180072.0
45	185372.0
46	190672.0
47	192119.5
48	193567.0
49	192212.5
50	190858.0
51	166463.0
52	142068.0
53	122223.0
54	102378.0
55	91884.0
56	81390.0
57	75985.0
58	70580.0
59	65011.5
60	59443.0
61	54467.0
62	49491.0
63	44772.5
64	40054.0
65	34506.0
66	28958.0
67	25273.0
68	21588.0
69	19627.5
70	17667.0
71	14983.0
72	12299.0
73	9899.0
74	7499.0
75	5691.0
76	3883.0
77	3156.0
78	2429.0
79	1891.0
80	1353.0
81	1030.5
82	708.0
83	480.0
84	252.0
85	179.5
86	107.0
87	93.5
88	80.0
89	63.0
90	46.0
91	63.5
92	81.0
93	44.0
94	7.0
95	7.0
96	7.0
97	5.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2750853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.004509287596676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45145107401882	18.59184076644124
2	8.960059598883912	4.301636677176572
3	3.5293424999624596	2.5416040445837553
4	1.8529063479391243	1.7791243055260617
5	1.2050453479307717	1.446326112318969
6	0.8041373194633026	1.1581753052135964
7	0.606971412359472	1.0199035633702032
8	0.46197743094912697	0.8871633225502695
9	0.3749863277813738	0.8101226509154784
>10	3.4803714899321103	18.9153916789256
>50	0.7879317987280662	12.898097773745828
>100	0.4106209129940981	19.898664321855954
>500	0.06104160244108408	10.239545701716084
>1k	0.013003850144090593	4.025463108284379
>5k	0.0	0.0
>10k+	1.5298647228341873E-4	1.48694066737613
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40490	1.471907077550127	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2925	0.10633065452788644	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2862	0.10404045581497812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.635236052235434E-5	0.0	0.0	0.04642196438704649	0.0
2	3.635236052235434E-5	0.0	0.0	0.2564295511246875	0.0
3	3.635236052235434E-5	0.0	0.0	0.41299916789446767	0.0
4	3.635236052235434E-5	0.0	0.0	0.6906948499247324	0.0
5	3.635236052235434E-5	0.0	0.0	1.3196997440430296	0.0
6	3.635236052235434E-5	0.0	0.0	1.9727335484666029	0.0
7	3.635236052235434E-5	0.0	0.0	2.3775897876040633	0.0
8	3.635236052235434E-5	0.0	0.0	3.2950143101067195	0.0
9	3.635236052235434E-5	0.0	0.0	3.7384767561189203	0.0
10	3.635236052235434E-5	0.0	0.0	4.534920622803181	0.0
11	3.635236052235434E-5	0.0	0.0	5.386729134562988	0.0
12	3.635236052235434E-5	0.0	0.0	6.074515795645932	0.0
13	3.635236052235434E-5	0.0	0.0	6.34036060814591	0.0
14	3.635236052235434E-5	0.0	0.0	6.437530467822163	0.0
15	3.635236052235434E-5	0.0	0.0	6.580686063559194	0.0
16	3.635236052235434E-5	0.0	0.0	6.895679267485394	0.0
17	3.635236052235434E-5	0.0	0.0	7.260911433653488	0.0
18	3.635236052235434E-5	0.0	0.0	7.769153786116525	0.0
19	3.635236052235434E-5	0.0	0.0	8.031617829087923	0.0
20	3.635236052235434E-5	0.0	0.0	8.325199492666457	0.0
21	3.635236052235434E-5	0.0	0.0	8.708317020211549	0.0
22	3.635236052235434E-5	0.0	0.0	9.113900306559456	0.0
23	3.635236052235434E-5	0.0	0.0	9.557217343129567	0.0
24	3.635236052235434E-5	0.0	0.0	9.903037348778724	0.0
25	3.635236052235434E-5	0.0	0.0	10.163574716642438	0.0
26	3.635236052235434E-5	0.0	0.0	10.445959853180087	0.0
27	3.635236052235434E-5	0.0	0.0	10.772549460112918	0.0
28	3.635236052235434E-5	0.0	0.0	11.084089189789495	0.0
29	3.635236052235434E-5	0.0	0.0	11.47033302033951	0.0
30	7.270472104470868E-5	0.0	0.0	11.881187398963158	0.0
31	7.270472104470868E-5	0.0	0.0	12.22893407972	0.0
32	7.270472104470868E-5	0.0	0.0	12.532730756605314	0.0
33	7.270472104470868E-5	0.0	0.0	12.863319123195605	0.0
34	7.270472104470868E-5	0.0	0.0	13.202086770903426	0.0
35	7.270472104470868E-5	0.0	0.0	13.628754426354298	0.0
36	7.270472104470868E-5	0.0	0.0	13.936949738862818	0.0
37	7.270472104470868E-5	0.0	0.0	14.273354483136686	0.0
38	7.270472104470868E-5	0.0	0.0	14.66454223471774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	50	2.7284841E-11	44.0	40
CGGCATA	75	0.0	44.0	33
CATTACG	70	0.0	44.0	21
TACGCCC	30	2.5290083E-6	44.0	17
TATTACG	30	2.5290083E-6	44.0	1
CAATACG	30	2.5290083E-6	44.0	14
CGCCGTT	165	0.0	42.666668	26
TACGCGG	275	0.0	42.4	2
CGTTTTT	27550	0.0	42.299095	1
CATACGA	590	0.0	42.135593	18
GCCCTAC	4135	0.0	41.392986	26
CTACGGG	565	0.0	41.274338	3
CCCTAGC	4260	0.0	41.21127	21
ACTACGG	225	0.0	41.066666	2
TACTACG	145	0.0	40.96552	1
TCCTAGC	4375	0.0	40.832	15
ATACGAA	605	0.0	40.727272	19
ACATACG	605	0.0	40.727272	17
GTACGAG	200	0.0	40.7	1
ATCCTAG	4395	0.0	40.59613	14
>>END_MODULE