SRR1546201_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546201_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1735393
Sequences flagged as poor quality	0
Sequence length	50
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24494	1.4114382160121655	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3991	0.2299767257330184	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3359	0.19355846197374313	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2894	0.16676337866984595	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2376	0.13691423210765516	No Hit
TATAGGGTTTATCTATCTTGTTGATTTTCTCAAAGAACCAACTCCTCGTT	2305	0.13282293981824292	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	2224	0.1281554091782092	TruSeq Adapter, Index 13 (95% over 21bp)
TAATTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2165	0.12475560290954267	No Hit
TTAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2126	0.12250827334211904	No Hit
TTGTTAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT	2102	0.12112530130062758	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2074	0.11951183391888753	No Hit
TTTTTTTTGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA	2057	0.11853222872283108	No Hit
TTAGTATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1911	0.110119148803758	No Hit
TCTATAGGGATTATTTTGGAATTTTACAATATAGGTGATATTATGGCATT	1883	0.10850568142201795	No Hit
GGCTAAGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1868	0.10764132389608579	No Hit
ATTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1835	0.10573973733903502	No Hit
TGTATAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTA	1832	0.10556686583384858	No Hit
ATTAAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1809	0.10424151762741926	No Hit
GGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1799	0.1036652792767978	No Hit
AGTTAAGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT	1795	0.10343478393654924	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1765	0.1017060688846849	No Hit
AGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1744	0.10049596834837987	No Hit
TGTTGAGGGGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTAT	1743	0.10043834451331772	No Hit
AATGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGT	1741	0.10032309684319343	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1739	0.10020784917306916	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	35	1.4466605E-7	44.0	42
TCACGAG	20	7.8575936E-4	44.0	1
CTAGATT	20	7.8575936E-4	44.0	28
CAGCGTG	20	7.8575936E-4	44.0	1
AAGTAGT	45	4.802132E-10	44.0	15
AGACTAC	20	7.8575936E-4	44.0	23
AATCCGG	30	2.5284662E-6	44.0	2
ATCTCGC	45	4.802132E-10	44.0	19
ACCGGAC	25	4.4437824E-5	44.0	41
GCGACAA	25	4.4437824E-5	44.0	8
CTCCGAT	50	2.7284841E-11	44.0	21
CTCCGAC	25	4.4437824E-5	44.0	16
CGACGTC	35	1.4466605E-7	44.0	18
CCTAGTT	40	8.3164196E-9	44.0	34
TGCCAAC	20	7.8575936E-4	44.0	21
ACGTTGA	20	7.8575936E-4	44.0	21
ACGTTAG	30	2.5284662E-6	44.0	1
CGTGCCG	25	4.4437824E-5	44.0	40
GAGTCGA	30	2.5284662E-6	44.0	44
TCCGAGT	20	7.8575936E-4	44.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content