##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546201_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1735393
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872709524586075	31.0	31.0	34.0	30.0	34.0
2	32.218235869339104	33.0	31.0	34.0	30.0	34.0
3	32.06940560437895	33.0	31.0	34.0	30.0	34.0
4	35.86576066631593	37.0	35.0	37.0	35.0	37.0
5	35.876773157434656	37.0	35.0	37.0	35.0	37.0
6	35.92940446342702	37.0	35.0	37.0	35.0	37.0
7	36.16640207722401	37.0	35.0	37.0	35.0	37.0
8	36.04760708381329	37.0	35.0	37.0	35.0	37.0
9	37.83282633962451	39.0	38.0	39.0	35.0	39.0
10	37.5017364942696	39.0	37.0	39.0	35.0	39.0
11	37.346557235162294	39.0	37.0	39.0	34.0	39.0
12	36.63167247995123	39.0	35.0	39.0	33.0	39.0
13	36.309392166500615	39.0	35.0	39.0	33.0	39.0
14	37.4503262373422	40.0	35.0	41.0	33.0	41.0
15	37.66926511746907	40.0	35.0	41.0	33.0	41.0
16	37.83200519997487	40.0	35.0	41.0	34.0	41.0
17	37.77201072033827	40.0	35.0	41.0	34.0	41.0
18	37.55298367574377	39.0	36.0	41.0	34.0	41.0
19	37.22806476688566	37.0	35.0	41.0	34.0	41.0
20	36.855753134880686	37.0	35.0	41.0	33.0	41.0
21	36.73171149128756	37.0	35.0	41.0	33.0	41.0
22	36.64262273732809	37.0	35.0	41.0	33.0	41.0
23	36.611222933364374	36.0	35.0	41.0	33.0	41.0
24	36.54961210515428	36.0	35.0	40.0	33.0	41.0
25	36.509265624558815	36.0	35.0	40.0	33.0	41.0
26	36.42812953607627	36.0	35.0	40.0	33.0	41.0
27	36.3265784753079	36.0	35.0	40.0	32.0	41.0
28	36.22245566278071	36.0	35.0	40.0	32.0	41.0
29	36.10304812800328	36.0	35.0	40.0	31.0	41.0
30	35.82886009105719	36.0	35.0	40.0	31.0	41.0
31	35.43222659074919	36.0	35.0	40.0	30.0	41.0
32	34.946325126354665	36.0	35.0	40.0	25.0	41.0
33	34.408391067614076	36.0	34.0	40.0	21.0	41.0
34	33.83235728160711	36.0	34.0	40.0	18.0	41.0
35	33.506640282633384	36.0	33.0	40.0	15.0	41.0
36	33.275539315878305	36.0	33.0	40.0	15.0	41.0
37	33.21623632226245	36.0	33.0	40.0	13.0	41.0
38	33.17856474009057	35.0	33.0	40.0	12.0	41.0
39	33.11005057644003	36.0	33.0	40.0	12.0	41.0
40	32.89422280716818	35.0	33.0	40.0	10.0	41.0
41	32.78780483729046	35.0	33.0	40.0	10.0	41.0
42	32.65307109110155	35.0	33.0	40.0	10.0	41.0
43	32.461305306636596	35.0	32.0	40.0	10.0	41.0
44	32.30098715391845	35.0	32.0	40.0	10.0	41.0
45	32.23002685846952	35.0	32.0	40.0	10.0	41.0
46	32.10621052407149	35.0	31.0	40.0	10.0	41.0
47	32.06764980612461	35.0	31.0	40.0	10.0	41.0
48	31.99720812519124	35.0	31.0	40.0	8.0	41.0
49	31.887929708141037	35.0	31.0	40.0	8.0	41.0
50	31.664178085309782	35.0	31.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	8.0
14	10.0
15	42.0
16	126.0
17	316.0
18	813.0
19	1687.0
20	3009.0
21	5118.0
22	8672.0
23	13869.0
24	21953.0
25	35854.0
26	54597.0
27	63278.0
28	56820.0
29	47715.0
30	44929.0
31	45407.0
32	53028.0
33	67820.0
34	124099.0
35	269897.0
36	83803.0
37	104161.0
38	176928.0
39	451426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.171136451512712	22.155961214549098	27.481844170167797	23.191058163770396
2	37.616666657062694	23.69774454547183	27.734351815410115	10.951236982055361
3	22.08992429956788	24.45624708639484	43.01913168947898	10.434696924558299
4	21.258412359621133	26.699370113858933	41.499360663549986	10.542856862969945
5	17.74422277835626	30.2217999035377	40.54804877050904	11.485928547596998
6	15.42636163681656	39.48010623530232	36.4757723466673	8.617759781213822
7	74.0406927998442	4.931274933113134	19.36351016743758	1.664522099605104
8	74.12436260835442	4.150356720350952	18.59290662115152	3.1323740501431088
9	70.80229089318672	5.190582190892783	20.93692898380943	3.070197932111055
10	41.607866344972	23.233296434870947	26.354664332517185	8.804172887639862
11	36.140977864956234	19.126503333826978	33.765953879034896	10.966564922181892
12	34.488614394549245	19.397796349299554	35.48383564990754	10.629753606243659
13	16.182443976666956	38.78078337298814	33.33873076588416	11.698041884460753
14	10.108027403591002	40.55692284110861	36.00175867944609	13.333291075854287
15	8.284866886059815	24.156257401061314	56.17759204975472	11.381283663124146
16	9.121910714172525	19.618149894577193	53.32769003908625	17.932249352164035
17	9.412853457401292	21.158953620303876	36.87516314748302	32.55302977481181
18	12.26794161322536	24.34416872719897	47.096363763136075	16.291525896439595
19	17.760818442854152	25.70956549899648	36.274953281475725	20.25466277667364
20	20.576837638506092	22.344506402872433	40.024420981299336	17.05423497732214
21	13.97994575292167	27.961274477884835	41.6759200941804	16.382859675013094
22	18.29574050373604	23.470188020811424	34.01068230654382	24.223389168908714
23	12.61668106302146	26.608324454460746	34.607031375601956	26.167963106915842
24	11.320721012473832	22.89827145781964	51.36876776614865	14.41223976355788
25	11.402892601272448	26.120365819154507	45.51989088350592	16.956850696067114
26	11.76165859836936	30.25810291962685	38.266433021223435	19.713805460780353
27	11.85835139360364	36.11988754132349	34.7877973461919	17.23396371888097
28	10.76119357402041	31.636234558973097	42.513136793798296	15.089435073208202
29	11.513415116921642	22.899769677531257	40.338874249233456	25.24794095631364
30	13.300214994528616	32.89410525454465	36.30376519900679	17.50191455191994
31	18.741806610952104	32.893586640029085	31.816251419707235	16.548355329311573
32	20.51252943857674	26.542460410984713	35.35792757029675	17.58708258014179
33	19.640450318746243	31.480534956635182	29.785875591292577	19.093139133325995
34	15.326326659148675	29.139220914225195	31.916171149704997	23.618281276921135
35	16.21079490351753	25.81409513579921	33.48797649869511	24.48713346198815
36	25.727428887865745	29.138183685194075	29.555149755703752	15.579237671236429
37	14.186642449289586	33.05107258125393	35.65025328556701	17.11203168388947
38	13.524371712920358	31.20371005299664	34.38708119716975	20.884837036913254
39	14.743922558175585	31.854052655508003	35.32122118736217	18.080803598954244
40	18.13502762774772	25.717978578915552	33.92176872904293	22.225225064293795
41	12.907681430085288	26.63552290461008	35.40696545393464	25.04983021136999
42	17.426484951823593	25.24408015936448	32.464346692651176	24.86508819616075
43	16.504100223983848	25.196713366943396	34.74423372688492	23.55495268218784
44	14.382563488500876	30.013893106633482	31.827603315214482	23.77594008965116
45	14.718971437593675	37.38478834477262	26.530878020137227	21.365362197496474
46	20.47104027733199	32.48762672201628	30.281440572827016	16.759892427824706
47	16.788128107005157	30.50473293369283	31.43132420149211	21.2758147578099
48	17.592672092142816	27.202944808467016	33.434098212912005	21.770284886478166
49	19.876131804150415	25.088726300036935	34.38356614323096	20.651575752581692
50	17.332039486156738	30.80864103981058	31.661992413245876	20.197327060786808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4924.0
1	5327.0
2	5730.0
3	22124.0
4	38518.0
5	30021.5
6	21525.0
7	24126.5
8	26728.0
9	29997.0
10	33266.0
11	33291.0
12	33316.0
13	31383.0
14	29450.0
15	27182.5
16	24915.0
17	22457.0
18	19999.0
19	18245.0
20	16491.0
21	16906.0
22	17321.0
23	16243.5
24	15166.0
25	17851.0
26	20536.0
27	23978.5
28	27421.0
29	30153.5
30	32886.0
31	46069.0
32	59252.0
33	72470.0
34	85688.0
35	86185.5
36	86683.0
37	88951.5
38	91220.0
39	104067.0
40	116914.0
41	134288.0
42	151662.0
43	162260.0
44	172858.0
45	167124.5
46	161391.0
47	150598.5
48	139806.0
49	123821.5
50	107837.0
51	87052.5
52	66268.0
53	52950.5
54	39633.0
55	32483.5
56	25334.0
57	21228.5
58	17123.0
59	15479.0
60	13835.0
61	12444.5
62	11054.0
63	8949.5
64	6845.0
65	5645.0
66	4445.0
67	3534.5
68	2624.0
69	2580.5
70	2537.0
71	2063.0
72	1589.0
73	1390.5
74	1192.0
75	974.5
76	757.0
77	539.5
78	322.0
79	210.0
80	98.0
81	78.0
82	58.0
83	69.0
84	80.0
85	80.0
86	80.0
87	44.5
88	9.0
89	5.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1735393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.00308493961198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93334918785843	18.157105359363598
2	8.72131866892788	4.012344682535435
3	3.568586949564904	2.462655261456969
4	1.9922032454311507	1.8330728188649368
5	1.20022613538443	1.3804451869495136
6	0.8211948269152215	1.1334008613300477
7	0.5499390628746276	0.885520648244097
8	0.41311662986946796	0.7602365541482892
9	0.3313163883567242	0.6859169120929677
>10	2.257553569430731	10.595425829625164
>50	0.5744145288155574	9.57668059832066
>100	0.527632753645182	24.009461466598733
>500	0.07695692210888662	12.5336931481893
>1k	0.03193963760728301	10.557033462758467
>5k	0.0	0.0
>10k+	2.5149320950616544E-4	1.4170072095218584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24494	1.4114382160121655	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3991	0.2299767257330184	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3359	0.19355846197374313	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2894	0.16676337866984595	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2376	0.13691423210765516	No Hit
TATAGGGTTTATCTATCTTGTTGATTTTCTCAAAGAACCAACTCCTCGTT	2305	0.13282293981824292	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	2224	0.1281554091782092	TruSeq Adapter, Index 13 (95% over 21bp)
TAATTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2165	0.12475560290954267	No Hit
TTAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2126	0.12250827334211904	No Hit
TTGTTAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT	2102	0.12112530130062758	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2074	0.11951183391888753	No Hit
TTTTTTTTGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA	2057	0.11853222872283108	No Hit
TTAGTATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1911	0.110119148803758	No Hit
TCTATAGGGATTATTTTGGAATTTTACAATATAGGTGATATTATGGCATT	1883	0.10850568142201795	No Hit
GGCTAAGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1868	0.10764132389608579	No Hit
ATTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1835	0.10573973733903502	No Hit
TGTATAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTA	1832	0.10556686583384858	No Hit
ATTAAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1809	0.10424151762741926	No Hit
GGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1799	0.1036652792767978	No Hit
AGTTAAGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT	1795	0.10343478393654924	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1765	0.1017060688846849	No Hit
AGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1744	0.10049596834837987	No Hit
TGTTGAGGGGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTAT	1743	0.10043834451331772	No Hit
AATGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGT	1741	0.10032309684319343	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1739	0.10020784917306916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13870057099458163	0.0
2	0.0	0.0	0.0	0.5490975243071742	0.0
3	0.0	0.0	0.0	0.7865077247632093	0.0
4	0.0	0.0	0.0	1.3167046311700001	0.0
5	0.0	0.0	0.0	2.197196830919567	0.0
6	0.0	0.0	0.0	3.0992403449823756	0.0
7	0.0	0.0	0.0	3.656174710858002	0.0
8	0.0	0.0	0.0	5.090489589389838	0.0
9	0.0	0.0	0.0	5.60512806032985	0.0
10	0.0	0.0	0.0	6.496799284081473	0.0
11	0.0	0.0	0.0	7.293275932310434	0.0
12	0.0	0.0	0.0	8.01144178868994	0.0
13	0.0	0.0	0.0	8.272477761521454	0.0
14	0.0	0.0	0.0	8.357472918238116	0.0
15	0.0	0.0	0.0	8.495597250882076	0.0
16	0.0	0.0	0.0	8.848427992967586	0.0
17	0.0	0.0	0.0	9.241653043431661	0.0
18	0.0	0.0	0.0	9.933254311847518	0.0
19	0.0	0.0	0.0	10.182016407810796	0.0
20	0.0	0.0	0.0	10.426860082989847	0.0
21	0.0	0.0	0.0	10.782917759838838	0.0
22	0.0	0.0	0.0	11.091781515771931	0.0
23	0.0	0.0	0.0	11.530241276759789	0.0
24	0.0	0.0	0.0	11.82936660456738	0.0
25	0.0	0.0	0.0	12.04891341615415	0.0
26	0.0	0.0	0.0	12.327755154019867	0.0
27	0.0	0.0	0.0	12.551566129401236	0.0
28	0.0	0.0	0.0	12.769902840451701	0.0
29	0.0	0.0	0.0	13.033589509696075	0.0
30	0.0	0.0	0.0	13.460294008331253	0.0
31	0.0	0.0	0.0	13.856457874383498	0.0
32	0.0	0.0	0.0	14.156101816706649	0.0
33	0.0	0.0	0.0	14.440302571233143	0.0
34	0.0	0.0	0.0	14.72692352683225	0.0
35	0.0	0.0	0.0	15.164000315778615	0.0
36	0.0	0.0	0.0	15.47660962099075	0.0
37	0.0	0.0	0.0	15.792964475481922	0.0
38	0.0	0.0	0.0	16.083215732689943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	35	1.4466605E-7	44.0	42
TCACGAG	20	7.8575936E-4	44.0	1
CTAGATT	20	7.8575936E-4	44.0	28
CAGCGTG	20	7.8575936E-4	44.0	1
AAGTAGT	45	4.802132E-10	44.0	15
AGACTAC	20	7.8575936E-4	44.0	23
AATCCGG	30	2.5284662E-6	44.0	2
ATCTCGC	45	4.802132E-10	44.0	19
ACCGGAC	25	4.4437824E-5	44.0	41
GCGACAA	25	4.4437824E-5	44.0	8
CTCCGAT	50	2.7284841E-11	44.0	21
CTCCGAC	25	4.4437824E-5	44.0	16
CGACGTC	35	1.4466605E-7	44.0	18
CCTAGTT	40	8.3164196E-9	44.0	34
TGCCAAC	20	7.8575936E-4	44.0	21
ACGTTGA	20	7.8575936E-4	44.0	21
ACGTTAG	30	2.5284662E-6	44.0	1
CGTGCCG	25	4.4437824E-5	44.0	40
GAGTCGA	30	2.5284662E-6	44.0	44
TCCGAGT	20	7.8575936E-4	44.0	4
>>END_MODULE