SRR1546200_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546200_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1784950
Sequences flagged as poor quality	0
Sequence length	50
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24860	1.3927560996106334	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4172	0.2337320373119695	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3579	0.20050981820219052	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2928	0.16403820835317515	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2540	0.14230090478724894	No Hit
TATAGGGTTTATCTATCTTGTTGATTTTCTCAAAGAACCAACTCCTCGTT	2346	0.13143225300428582	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	2283	0.12790274237373594	TruSeq Adapter, Index 13 (95% over 21bp)
TAATTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2282	0.12784671839547326	No Hit
TTAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2145	0.12017143337348386	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2114	0.11843469004734027	No Hit
TTGTTAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT	2072	0.116081682960307	No Hit
TTTTTTTTGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA	2029	0.11367265189501105	No Hit
ATTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1932	0.1082383260035295	No Hit
TTAGTATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1912	0.10711784643827559	No Hit
ATTAAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1903	0.10661363063391133	No Hit
TGTATAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTA	1879	0.1052690551556066	No Hit
TCTATAGGGATTATTTTGGAATTTTACAATATAGGTGATATTATGGCATT	1870	0.10476483935124234	No Hit
AGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1846	0.10342026387293761	No Hit
GGCTAAGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1844	0.10330821591641223	No Hit
AGTTAAGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT	1801	0.10089918485111626	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1789	0.10022689711196393	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACG	190	0.0	44.000004	1
TAGTACG	175	0.0	44.000004	1
TGATCAC	25	4.4438326E-5	44.0	33
TCACGAG	25	4.4438326E-5	44.0	1
TGTTTCG	25	4.4438326E-5	44.0	1
AACCGGC	20	7.857653E-4	44.0	34
AACCGCA	35	1.4466787E-7	44.0	10
ATCTCGA	50	2.7284841E-11	44.0	39
TCGTTAG	75	0.0	44.0	1
AGTCCGA	25	4.4438326E-5	44.0	34
GGTACGA	30	2.528508E-6	44.0	8
GTCGCAT	20	7.857653E-4	44.0	29
CGAACTC	55	1.8189894E-12	44.0	13
ACGCCCA	25	4.4438326E-5	44.0	16
GCGACAA	20	7.857653E-4	44.0	8
GGTAATC	60	0.0	44.0	8
CTCCGAT	30	2.528508E-6	44.0	21
CCTAGTT	40	8.3164196E-9	44.0	34
CACGGAT	20	7.857653E-4	44.0	23
CGACGGT	105	0.0	44.0	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content