##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546200_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1784950
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.806828762710442	31.0	31.0	34.0	30.0	34.0
2	32.18556037984257	33.0	31.0	34.0	30.0	34.0
3	32.16995658141685	33.0	31.0	34.0	30.0	34.0
4	35.9039760217373	37.0	35.0	37.0	35.0	37.0
5	35.83990139779826	37.0	35.0	37.0	35.0	37.0
6	35.918225160368635	37.0	35.0	37.0	35.0	37.0
7	36.14851060253789	37.0	35.0	37.0	35.0	37.0
8	36.02905123392812	37.0	35.0	37.0	35.0	37.0
9	37.80617608336368	39.0	38.0	39.0	35.0	39.0
10	37.493944928429364	39.0	37.0	39.0	35.0	39.0
11	37.33418863273481	39.0	37.0	39.0	34.0	39.0
12	36.606750889380656	39.0	35.0	39.0	33.0	39.0
13	36.22728871957197	39.0	35.0	39.0	32.0	39.0
14	37.35730804784448	40.0	35.0	41.0	33.0	41.0
15	37.613321941791085	40.0	35.0	41.0	33.0	41.0
16	37.77954900697498	40.0	35.0	41.0	33.0	41.0
17	37.72647413092804	40.0	35.0	41.0	33.0	41.0
18	37.51645312193619	39.0	36.0	41.0	33.0	41.0
19	37.202671223283566	37.0	35.0	41.0	33.0	41.0
20	36.83894394800975	37.0	35.0	41.0	33.0	41.0
21	36.71535337124289	37.0	35.0	41.0	33.0	41.0
22	36.630271996414464	37.0	35.0	41.0	33.0	41.0
23	36.587107201882404	36.0	35.0	40.0	33.0	41.0
24	36.52734642426959	36.0	35.0	40.0	33.0	41.0
25	36.48228745903246	36.0	35.0	40.0	33.0	41.0
26	36.411866999075606	36.0	35.0	40.0	33.0	41.0
27	36.30188464662876	36.0	35.0	40.0	32.0	41.0
28	36.192419395501275	36.0	35.0	40.0	32.0	41.0
29	36.07234264265105	36.0	35.0	40.0	31.0	41.0
30	35.80271548222639	36.0	35.0	40.0	31.0	41.0
31	35.41806773298972	36.0	35.0	40.0	30.0	41.0
32	34.95143561444298	36.0	35.0	40.0	25.0	41.0
33	34.401770357713104	36.0	34.0	40.0	21.0	41.0
34	33.90246225384465	36.0	34.0	40.0	18.0	41.0
35	33.544880248746466	36.0	33.0	40.0	15.0	41.0
36	33.30143141264461	36.0	33.0	40.0	15.0	41.0
37	33.1803030897224	36.0	33.0	40.0	14.0	41.0
38	33.148282024706575	35.0	33.0	40.0	12.0	41.0
39	33.09639653771814	36.0	33.0	40.0	12.0	41.0
40	32.889159360206165	35.0	33.0	40.0	10.0	41.0
41	32.81654892293902	35.0	33.0	40.0	10.0	41.0
42	32.66824953079918	35.0	33.0	40.0	10.0	41.0
43	32.50229138071094	35.0	32.0	40.0	10.0	41.0
44	32.336998235244685	35.0	32.0	40.0	10.0	41.0
45	32.26291548782879	35.0	32.0	40.0	10.0	41.0
46	32.15507437183114	35.0	31.0	40.0	10.0	41.0
47	32.10260511498922	35.0	31.0	40.0	10.0	41.0
48	32.0380845401832	35.0	31.0	40.0	9.0	41.0
49	31.918369702232557	35.0	31.0	40.0	8.0	41.0
50	31.667813104008516	35.0	31.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	5.0
14	24.0
15	41.0
16	114.0
17	361.0
18	879.0
19	1753.0
20	3192.0
21	5457.0
22	9084.0
23	14088.0
24	22073.0
25	36866.0
26	54591.0
27	64113.0
28	58960.0
29	49828.0
30	46655.0
31	47781.0
32	55859.0
33	71046.0
34	131015.0
35	272607.0
36	87111.0
37	108444.0
38	183249.0
39	459747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.422728927981176	22.161573153309615	27.524972688310594	22.890725230398612
2	37.59035267094316	23.689291016555085	27.712316871621052	11.008039440880697
3	22.088125717807223	24.497604974929267	42.917224572116865	10.497044735146643
4	21.331129723521666	26.725902686349755	41.371747107762125	10.571220482366453
5	17.843805148603604	30.26745847222611	40.40959130507857	11.479145074091711
6	15.49886551444018	39.4896775820051	36.3042662259447	8.707190677610017
7	74.2484103196168	4.9262444326171595	19.181937869408106	1.6434073783579373
8	74.28908372783552	4.187512255245245	18.421692484383318	3.1017115325359255
9	70.94977450348749	5.19134989775624	20.768144765959832	3.090730832796437
10	41.721280708143084	23.289784027563798	26.116753970699456	8.872181293593657
11	36.21524412448528	19.207036611669796	33.54939914283313	11.028320121011793
12	34.549651250735316	19.385472982436482	35.339533320261076	10.725342446567131
13	16.29933611585759	38.71783523347993	33.25471301717135	11.728115633491134
14	10.183366480853806	40.60898064371551	35.86010812627804	13.347544749152638
15	8.359057676685621	24.16230146502703	56.03462281856635	11.444018039721
16	9.221658869996359	19.601557466595704	53.25891481554105	17.917868847866885
17	9.473150508417602	21.177343903190565	36.75722009019861	32.59228549819323
18	12.341690243424186	24.249474775203787	47.00047620381523	16.408358777556796
19	17.81254376873302	25.627104400683493	36.31037283957534	20.249978991008152
20	20.632398666629317	22.36858175299028	39.919549567214766	17.079470013165636
21	13.982016302977673	28.02201742345724	41.552144317768004	16.44382195579708
22	18.269923527269672	23.435502395025072	33.97193198689039	24.32264209081487
23	12.673576290652399	26.56701868399675	34.50735314714698	26.25205187820387
24	11.338020672847978	22.852292781310403	51.265301549063004	14.544384996778623
25	11.505812487744754	26.08493235104625	45.38805008543657	17.021205075772432
26	11.69371691083784	30.260511498921538	38.24286394576879	19.802907644471833
27	11.923583293649683	36.141460545113304	34.68226000728312	17.25269615395389
28	10.722765343567046	31.67203563125017	42.45183338468865	15.153365640494131
29	11.565702120507577	22.87559875626768	40.24230370598616	25.31639541723858
30	13.33101767556514	32.82971511807054	36.30376201014034	17.535505196223983
31	18.736995434045774	32.847082551331965	31.839491302277374	16.576430712344884
32	20.529650690495533	26.542144037648114	35.32782430880417	17.60038096305219
33	19.680775371859156	31.449900557438582	29.73013249670859	19.13919157399367
34	15.402728367741394	29.150564441580997	31.722289139751815	23.724418050925795
35	16.203815232919688	25.73226140788257	33.274265385585025	24.789657973612705
36	25.81484075184179	29.076220622426398	29.378413961175383	15.73052466455643
37	14.201294153897868	33.0446791226645	35.52883834281072	17.225188380626907
38	13.505028152049078	31.240763046583936	34.18527129611474	21.068937505252247
39	14.784951959438638	31.802963668450097	35.21605647217009	18.196027899941175
40	18.19221826941931	25.760049301100874	33.75595955068769	22.291772878792123
41	12.946861256617833	26.603378245889242	35.38933863693661	25.06042186055632
42	17.520322698114793	25.20569203619149	32.39267206364324	24.881313202050478
43	16.55329280932239	25.171629457407768	34.66018655984762	23.614891173422226
44	14.361466707750917	30.00106445558699	31.748508361578754	23.888960475083334
45	14.729768340849883	37.2997002717163	26.474635143841564	21.49589624359226
46	20.502703156951174	32.375920894142695	30.183310456875546	16.93806549203059
47	16.818734418331047	30.518053726995152	31.322894198717048	21.34031765595675
48	17.629961623574893	27.22457211686602	33.32894478836943	21.81652147118967
49	19.90375080534469	25.128042802319396	34.208073055267654	20.760133337068265
50	17.455558979243115	30.710776212218832	31.492310708983446	20.34135409955461
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5169.0
1	5599.0
2	6029.0
3	22609.5
4	39190.0
5	30529.0
6	21868.0
7	24404.5
8	26941.0
9	29968.0
10	32995.0
11	33448.5
12	33902.0
13	31876.0
14	29850.0
15	27485.0
16	25120.0
17	22811.0
18	20502.0
19	18638.0
20	16774.0
21	17328.5
22	17883.0
23	16670.0
24	15457.0
25	18159.0
26	20861.0
27	24611.0
28	28361.0
29	31050.0
30	33739.0
31	47075.5
32	60412.0
33	73708.5
34	87005.0
35	87760.5
36	88516.0
37	90834.5
38	93153.0
39	106455.0
40	119757.0
41	137434.0
42	155111.0
43	166880.0
44	178649.0
45	172479.0
46	166309.0
47	155906.5
48	145504.0
49	129288.5
50	113073.0
51	91364.5
52	69656.0
53	55729.5
54	41803.0
55	34334.0
56	26865.0
57	22291.0
58	17717.0
59	16051.5
60	14386.0
61	12900.0
62	11414.0
63	9192.5
64	6971.0
65	5846.0
66	4721.0
67	3628.5
68	2536.0
69	2538.5
70	2541.0
71	2056.0
72	1571.0
73	1390.5
74	1210.0
75	970.0
76	730.0
77	545.0
78	360.0
79	236.0
80	112.0
81	84.0
82	56.0
83	69.5
84	83.0
85	79.0
86	75.0
87	41.0
88	7.0
89	4.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1784950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.936760493213864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75582971308872	18.064036035734517
2	8.758946544381681	4.018037181226917
3	3.599295857634563	2.4766856109234228
4	1.9814190708917205	1.8178933866291898
5	1.2074493520711758	1.3847488298071413
6	0.8243731264213039	1.134506937466042
7	0.5827043121847985	0.935574447286195
8	0.43144563973991373	0.791677224364467
9	0.35411936165953883	0.7310115885955161
>10	2.3080553479502357	10.757057525633389
>50	0.5584853474736386	9.340113642137435
>100	0.530703271297477	24.226884358093336
>500	0.07325130837424852	11.8936980245714
>1k	0.03367593707138271	11.026448622979778
>5k	0.0	0.0
>10k+	2.458097596451293E-4	1.4016265845512745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24860	1.3927560996106334	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4172	0.2337320373119695	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3579	0.20050981820219052	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2928	0.16403820835317515	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2540	0.14230090478724894	No Hit
TATAGGGTTTATCTATCTTGTTGATTTTCTCAAAGAACCAACTCCTCGTT	2346	0.13143225300428582	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	2283	0.12790274237373594	TruSeq Adapter, Index 13 (95% over 21bp)
TAATTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	2282	0.12784671839547326	No Hit
TTAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2145	0.12017143337348386	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2114	0.11843469004734027	No Hit
TTGTTAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT	2072	0.116081682960307	No Hit
TTTTTTTTGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA	2029	0.11367265189501105	No Hit
ATTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1932	0.1082383260035295	No Hit
TTAGTATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1912	0.10711784643827559	No Hit
ATTAAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1903	0.10661363063391133	No Hit
TGTATAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTA	1879	0.1052690551556066	No Hit
TCTATAGGGATTATTTTGGAATTTTACAATATAGGTGATATTATGGCATT	1870	0.10476483935124234	No Hit
AGTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1846	0.10342026387293761	No Hit
GGCTAAGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1844	0.10330821591641223	No Hit
AGTTAAGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT	1801	0.10089918485111626	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	1789	0.10022689711196393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14028404156979188	0.0
2	0.0	0.0	0.0	0.5410795820611222	0.0
3	0.0	0.0	0.0	0.773635115829575	0.0
4	0.0	0.0	0.0	1.2849659654332055	0.0
5	0.0	0.0	0.0	2.1595562900921594	0.0
6	0.0	0.0	0.0	3.072747135774111	0.0
7	0.0	0.0	0.0	3.6208297151180706	0.0
8	0.0	0.0	0.0	5.05297067144738	0.0
9	0.0	0.0	0.0	5.579091851312362	0.0
10	0.0	0.0	0.0	6.4959242555813885	0.0
11	0.0	0.0	0.0	7.325583349673661	0.0
12	0.0	0.0	0.0	8.077481161937309	0.0
13	0.0	0.0	0.0	8.351270343707107	0.0
14	0.0	0.0	0.0	8.441693044623099	0.0
15	0.0	0.0	0.0	8.582929493823356	0.0
16	0.0	0.0	0.0	8.9449564413569	0.0
17	0.0	0.0	0.0	9.358693520826915	0.0
18	0.0	0.0	0.0	10.088741981568111	0.0
19	0.0	0.0	0.0	10.347572761141768	0.0
20	0.0	0.0	0.0	10.604050533628392	0.0
21	0.0	0.0	0.0	10.997002717162946	0.0
22	0.0	0.0	0.0	11.320765287543068	0.0
23	0.0	0.0	0.0	11.778985405753662	0.0
24	0.0	0.0	0.0	12.094792571220482	0.0
25	0.0	0.0	0.0	12.334407126250035	0.0
26	5.602397826269643E-5	0.0	0.0	12.62349085408555	0.0
27	1.1204795652539286E-4	0.0	0.0	12.859407826549763	0.0
28	1.1204795652539286E-4	0.0	0.0	13.106025378862153	0.0
29	1.1204795652539286E-4	0.0	0.0	13.384632622762542	0.0
30	1.1204795652539286E-4	0.0	0.0	13.82565337964649	0.0
31	1.1204795652539286E-4	0.0	0.0	14.251323566486457	0.0
32	1.1204795652539286E-4	0.0	0.0	14.563769293257515	0.0
33	1.1204795652539286E-4	0.0	0.0	14.86058433009328	0.0
34	1.1204795652539286E-4	0.0	0.0	15.159080086276926	0.0
35	1.1204795652539286E-4	0.0	0.0	15.61001708731337	0.0
36	1.1204795652539286E-4	0.0	0.0	15.940502535085017	0.0
37	1.680719347880893E-4	0.0	0.0	16.272724726182805	0.0
38	1.680719347880893E-4	0.0	0.0	16.57693492814925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACG	190	0.0	44.000004	1
TAGTACG	175	0.0	44.000004	1
TGATCAC	25	4.4438326E-5	44.0	33
TCACGAG	25	4.4438326E-5	44.0	1
TGTTTCG	25	4.4438326E-5	44.0	1
AACCGGC	20	7.857653E-4	44.0	34
AACCGCA	35	1.4466787E-7	44.0	10
ATCTCGA	50	2.7284841E-11	44.0	39
TCGTTAG	75	0.0	44.0	1
AGTCCGA	25	4.4438326E-5	44.0	34
GGTACGA	30	2.528508E-6	44.0	8
GTCGCAT	20	7.857653E-4	44.0	29
CGAACTC	55	1.8189894E-12	44.0	13
ACGCCCA	25	4.4438326E-5	44.0	16
GCGACAA	20	7.857653E-4	44.0	8
GGTAATC	60	0.0	44.0	8
CTCCGAT	30	2.528508E-6	44.0	21
CCTAGTT	40	8.3164196E-9	44.0	34
CACGGAT	20	7.857653E-4	44.0	23
CGACGGT	105	0.0	44.0	28
>>END_MODULE