FastQCFastQC Report
Thu 26 May 2016
SRR1546199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546199_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2439269
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378081.5499725532526343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60060.24622130646517462No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA37790.15492346272592322No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG31110.12753820919300002TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC29850.12237272723918519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC454.802132E-1044.00000432
CGGTAAT454.802132E-1044.00000429
TCTTGCG351.4469879E-744.01
CGTATTA254.4443157E-544.036
CCCTCGC207.858222E-444.021
GTCGAGT302.52889E-644.011
CGACGGT1600.044.028
ACGCATG502.7284841E-1144.01
GCGCGAT254.4443157E-544.025
GCAACAT207.858222E-444.016
CGGTTGC207.858222E-444.036
CACGCAC207.858222E-444.037
CGCGATT254.4443157E-544.026
AGGCGCC502.7284841E-1144.035
TGGCCGC207.858222E-444.037
CGTTATC551.8189894E-1244.032
CATTACG351.4469879E-744.044
CCGTATT254.4443157E-544.035
AATGCGT502.7284841E-1144.029
AGTGCGT207.858222E-444.021
Produced by FastQC (version 0.11.5)