##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546199_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2439269
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90473867375841	31.0	31.0	34.0	30.0	34.0
2	32.26145906827004	33.0	31.0	34.0	30.0	34.0
3	32.16486045614485	33.0	31.0	34.0	30.0	34.0
4	35.90415202259366	37.0	35.0	37.0	35.0	37.0
5	35.88839156321013	37.0	35.0	37.0	35.0	37.0
6	35.94535166068195	37.0	35.0	37.0	35.0	37.0
7	36.202292572077944	37.0	35.0	37.0	35.0	37.0
8	36.11208152934342	37.0	36.0	37.0	35.0	37.0
9	37.88263164087274	39.0	38.0	39.0	35.0	39.0
10	37.559686529038004	39.0	37.0	39.0	35.0	39.0
11	37.30174859763314	39.0	37.0	39.0	34.0	39.0
12	36.81614122919613	39.0	35.0	39.0	33.0	39.0
13	36.670744801003906	39.0	35.0	39.0	33.0	39.0
14	37.85448714348438	40.0	35.0	41.0	33.0	41.0
15	38.030043836903594	40.0	35.0	41.0	33.0	41.0
16	38.11105294250039	40.0	35.0	41.0	34.0	41.0
17	38.04824232177755	40.0	35.0	41.0	34.0	41.0
18	37.8595415265803	39.0	36.0	41.0	34.0	41.0
19	37.63297938849713	39.0	36.0	41.0	34.0	41.0
20	37.363636810864236	39.0	35.0	41.0	34.0	41.0
21	37.26530981207895	39.0	35.0	41.0	33.0	41.0
22	37.17167479273504	38.0	35.0	41.0	33.0	41.0
23	37.14882163467826	38.0	35.0	41.0	33.0	41.0
24	37.098378243645946	38.0	35.0	41.0	33.0	41.0
25	37.06424465690336	38.0	35.0	41.0	33.0	41.0
26	37.02789114279729	38.0	35.0	41.0	33.0	41.0
27	36.97428614884213	38.0	35.0	41.0	33.0	41.0
28	36.926015539901506	38.0	35.0	41.0	33.0	41.0
29	36.877806014834775	38.0	35.0	41.0	33.0	41.0
30	36.69770533713174	38.0	35.0	41.0	32.0	41.0
31	36.42185671199035	38.0	35.0	41.0	31.0	41.0
32	36.069231396783216	38.0	35.0	41.0	30.0	41.0
33	35.64674129831519	38.0	35.0	41.0	29.0	41.0
34	35.20343307769664	38.0	35.0	41.0	24.0	41.0
35	34.945615674204035	38.0	35.0	41.0	23.0	41.0
36	34.76575564236663	38.0	35.0	41.0	21.0	41.0
37	34.71038413557504	38.0	35.0	41.0	21.0	41.0
38	34.671426152671145	38.0	34.0	41.0	21.0	41.0
39	34.596330294034814	38.0	34.0	41.0	20.0	41.0
40	34.458637403254826	38.0	34.0	40.0	18.0	41.0
41	34.350386529734934	38.0	34.0	40.0	18.0	41.0
42	34.30064703810855	38.0	34.0	40.0	18.0	41.0
43	34.185502295974736	38.0	34.0	40.0	18.0	41.0
44	34.047858190302094	38.0	34.0	40.0	17.0	41.0
45	34.00268113110936	38.0	34.0	40.0	17.0	41.0
46	33.90457510016321	38.0	34.0	40.0	17.0	41.0
47	33.884993823969396	38.0	34.0	40.0	17.0	41.0
48	33.83415564253061	37.0	34.0	40.0	15.0	41.0
49	33.72124107673241	37.0	34.0	40.0	15.0	41.0
50	33.60821746187075	37.0	34.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	1.0
11	5.0
12	1.0
13	10.0
14	20.0
15	34.0
16	92.0
17	258.0
18	711.0
19	1491.0
20	2716.0
21	4712.0
22	7904.0
23	12573.0
24	20548.0
25	35438.0
26	55850.0
27	66620.0
28	60803.0
29	52380.0
30	51721.0
31	55894.0
32	67094.0
33	86605.0
34	153484.0
35	323940.0
36	140845.0
37	169918.0
38	290405.0
39	777192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.13352606867057	24.100416969182163	30.26095112921125	19.505105832936014
2	32.848242649744655	25.609721601020635	30.787994272054455	10.754041477180253
3	23.297102533586905	24.96776698264931	41.00384172471343	10.731288759050354
4	21.22976186718234	26.888957306471735	40.6575084584767	11.223772367869227
5	19.829506298813293	29.388476629678813	39.338465745270405	11.443551326237491
6	18.49185145221786	36.68972138784201	35.9481467603614	8.870280399578727
7	78.46592565231633	4.626017056749379	15.185656030556697	1.7224012603775967
8	79.67054884065678	3.817947098085533	14.212331645259296	2.2991724159983997
9	75.66016704184737	6.1094532829302555	15.766403787364167	2.4639758878582065
10	38.539086914973296	34.00871326614654	20.779504023541477	6.672695795338686
11	31.614389392887787	21.33622818967486	35.97614695222216	11.073235465215193
12	31.086444340497092	22.814047979128173	33.71846237540837	12.381045304966365
13	16.362197035259335	35.45373634478198	34.563469629630845	13.62059699032784
14	11.175356223524343	36.818202502471024	36.15931658213998	15.847124691864654
15	10.966605159168587	26.95336184734033	49.39041163561706	12.689621357874017
16	11.536161038409459	24.3814437850028	45.80704301165636	18.27535216493138
17	12.113383148804006	25.935761902438802	35.76218941002407	26.18866553873312
18	14.338066035357313	28.663300357607135	40.71252494087368	16.28610866616187
19	18.13272746876216	27.70047091977146	35.84102450365253	18.325777107813856
20	20.015299665596537	25.329678686524527	39.73141133675704	14.923610311121898
21	15.726104828946704	30.180927154815645	38.668838902146504	15.424129114091148
22	18.79755779292895	26.108764552003077	33.59420383729716	21.499473817770813
23	13.632485797999319	30.191545089942927	34.770703846111275	21.405265265946475
24	13.02164705901645	26.423817955297263	47.46147308886392	13.093061896822366
25	11.961124418832036	31.005149493557294	41.38489850852858	15.648827579082095
26	12.051151390027094	33.35860046595927	36.08019451729186	18.510053626721774
27	12.632842052270576	35.92572200933969	35.147538053408624	16.293897884981114
28	10.698656031786573	30.61618050325733	42.521960472584205	16.163202992371893
29	11.44933174651914	26.112167210750435	40.60749347447945	21.83100756825098
30	13.587882271287013	34.47139286400967	36.05612993072925	15.884594933974071
31	18.506445988531812	33.11582281412997	33.141404248567916	15.236326948770307
32	19.428607504953327	28.57597911505455	35.13753505660917	16.85787832338295
33	19.25888452647084	31.394364459188388	31.793541425730414	17.553209588610358
34	14.62495526323665	28.28675312152944	36.45379004939595	20.63450156583796
35	17.054330621182	28.150359800415615	35.64317834564372	19.152131232758666
36	22.38933057403673	29.707424642382612	32.50289328483246	15.400351498748192
37	15.264081165299931	31.37931896810069	38.11498444820969	15.24161541838969
38	14.382956533289276	32.515151055500645	36.29156931851305	16.810323092697033
39	16.708407313830495	31.903779369966983	35.64252241142736	15.74529090477516
40	18.99282120996085	28.957773824863104	32.89415804488968	19.155246920286366
41	13.750062006281391	28.026839188297803	36.588830506188536	21.63426829923227
42	16.539504253118455	27.496639362038383	33.45153814523941	22.51231823960375
43	18.479552685661154	28.037990069975884	32.32242938355712	21.160027860805837
44	14.833173381041615	31.29171075432845	32.328209803838774	21.546906060791162
45	15.254816094493883	36.82230209132326	29.157054838970197	18.765826975212658
46	18.895087011723593	34.27502255798766	30.832966761763466	15.996923668525284
47	16.214652832467433	29.855952746499053	35.112937523495766	18.816456897537744
48	16.699962160794893	28.108871961231007	36.4779366277356	18.713229250238495
49	17.87715909971389	28.821626479080415	34.191964887841394	19.1092495333643
50	16.240644225790597	31.18204675253119	34.106365472606754	18.470943549071464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7377.0
1	8561.0
2	9745.0
3	32983.0
4	56221.0
5	40939.0
6	25657.0
7	27586.5
8	29516.0
9	32814.5
10	36113.0
11	35929.5
12	35746.0
13	33975.0
14	32204.0
15	29908.0
16	27612.0
17	26250.0
18	24888.0
19	23639.5
20	22391.0
21	22956.5
22	23522.0
23	24218.5
24	24915.0
25	34632.0
26	44349.0
27	53426.5
28	62504.0
29	70232.0
30	77960.0
31	92184.0
32	106408.0
33	127814.0
34	149220.0
35	159748.0
36	170276.0
37	162776.5
38	155277.0
39	168777.0
40	182277.0
41	204888.5
42	227500.0
43	239405.0
44	251310.0
45	235795.0
46	220280.0
47	190826.0
48	161372.0
49	130489.0
50	99606.0
51	82340.0
52	65074.0
53	52804.5
54	40535.0
55	31969.0
56	23403.0
57	19291.5
58	15180.0
59	13374.0
60	11568.0
61	9613.5
62	7659.0
63	6339.5
64	5020.0
65	3973.5
66	2927.0
67	2299.0
68	1671.0
69	1211.0
70	751.0
71	666.0
72	581.0
73	436.5
74	292.0
75	265.0
76	238.0
77	163.0
78	88.0
79	53.5
80	19.0
81	12.0
82	5.0
83	5.5
84	6.0
85	3.0
86	0.0
87	2.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2439269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.980246249541672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32490805456764	15.803351197089139
2	10.045602447040663	4.21518426127823
3	4.198905073557391	2.6428218726505186
4	2.2489500266257987	1.8873410144609022
5	1.3316841863284021	1.3969531077895205
6	0.875337967055507	1.101888366023864
7	0.6479868514668896	0.9516446597168369
8	0.503963642242959	0.8458625052058559
9	0.38516492537940866	0.7272769483031704
>10	3.0128027271592415	13.460473680285059
>50	0.6981270425267462	10.368643489901684
>100	0.6196553964833522	26.85710642548201
>500	0.0858830794675726	12.124294013588926
>1k	0.020635522526533463	5.81060982887548
>5k	1.9652878596698538E-4	0.24764073282213198
>10k+	1.9652878596698538E-4	1.5589078965266676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37808	1.5499725532526343	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6006	0.24622130646517462	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3779	0.15492346272592322	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	3111	0.12753820919300002	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC	2985	0.12237272723918519	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10732723615148637	0.0
2	0.0	0.0	0.0	0.4801848422621695	0.0
3	0.0	0.0	0.0	0.6912726722636987	0.0
4	0.0	0.0	0.0	1.052692425476649	0.0
5	0.0	0.0	0.0	1.7706124252798687	0.0
6	0.0	0.0	0.0	2.312865042764861	0.0
7	0.0	0.0	0.0	2.6514090901823457	0.0
8	0.0	0.0	0.0	3.3489951292784848	0.0
9	0.0	0.0	0.0	3.5917317852192605	0.0
10	0.0	0.0	0.0	4.168010990177795	0.0
11	0.0	0.0	0.0	4.980139541805352	0.0
12	0.0	0.0	0.0	5.649561405486644	0.0
13	0.0	0.0	0.0	5.884057887834429	0.0
14	0.0	0.0	0.0	5.974412826137667	0.0
15	0.0	0.0	0.0	6.118964329067438	0.0
16	0.0	0.0	0.0	6.462796846104304	0.0
17	0.0	0.0	0.0	6.924246567311764	0.0
18	0.0	0.0	0.0	7.528484968242535	0.0
19	0.0	0.0	0.0	7.84636708784476	0.0
20	0.0	0.0	0.0	8.153508284654132	0.0
21	0.0	0.0	0.0	8.600240481882071	0.0
22	0.0	0.0	0.0	9.077473620170633	0.0
23	0.0	0.0	0.0	9.622308978632534	0.0
24	0.0	0.0	0.0	10.011851911371808	0.0
25	0.0	0.0	0.0	10.327233281774172	0.0
26	0.0	0.0	0.0	10.656348274831517	0.0
27	0.0	0.0	0.0	10.951272696861231	0.0
28	0.0	0.0	0.0	11.272557475210812	0.0
29	0.0	0.0	0.0	11.64463615943957	0.0
30	0.0	0.0	0.0	12.139005579130469	0.0
31	0.0	0.0	0.0	12.5939779499514	0.0
32	0.0	0.0	0.0	12.976715565195967	0.0
33	0.0	0.0	0.0	13.332641869346924	0.0
34	0.0	0.0	0.0	13.731859831777472	0.0
35	0.0	0.0	0.0	14.260624801938613	0.0
36	0.0	0.0	0.0	14.672551489811086	0.0
37	0.0	0.0	0.0	15.075500078097168	0.0
38	0.0	0.0	0.0	15.446102910339123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	45	4.802132E-10	44.000004	32
CGGTAAT	45	4.802132E-10	44.000004	29
TCTTGCG	35	1.4469879E-7	44.0	1
CGTATTA	25	4.4443157E-5	44.0	36
CCCTCGC	20	7.858222E-4	44.0	21
GTCGAGT	30	2.52889E-6	44.0	11
CGACGGT	160	0.0	44.0	28
ACGCATG	50	2.7284841E-11	44.0	1
GCGCGAT	25	4.4443157E-5	44.0	25
GCAACAT	20	7.858222E-4	44.0	16
CGGTTGC	20	7.858222E-4	44.0	36
CACGCAC	20	7.858222E-4	44.0	37
CGCGATT	25	4.4443157E-5	44.0	26
AGGCGCC	50	2.7284841E-11	44.0	35
TGGCCGC	20	7.858222E-4	44.0	37
CGTTATC	55	1.8189894E-12	44.0	32
CATTACG	35	1.4469879E-7	44.0	44
CCGTATT	25	4.4443157E-5	44.0	35
AATGCGT	50	2.7284841E-11	44.0	29
AGTGCGT	20	7.858222E-4	44.0	21
>>END_MODULE