FastQCFastQC Report
Thu 26 May 2016
SRR1546198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546198_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2497958
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389511.5593136473871858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61800.24740207801732453No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA39450.157928996404263No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC32500.13010627080199108No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG32200.12890528984074193TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGA207.858257E-444.00000416
GTCGCTA408.3200575E-944.00000422
GTACCGT408.3200575E-944.00000416
CCGATCA207.858257E-444.00000414
CGTTGCC207.858257E-444.00000418
CCGGATG650.044.0000041
ATAGCCG207.858257E-444.0000041
CGTCTGG207.858257E-444.0000042
CATTACG207.858257E-444.00000444
GTTCGCG207.858257E-444.0000041
TACGACG207.858257E-444.0000041
GTATCGC207.858257E-444.0000049
CGAGCCT207.858257E-444.00000414
GCGGCAA207.858257E-444.00000427
CCAACCG207.858257E-444.0000042
CACCGGT408.3200575E-944.00000433
ACTTTCG800.044.00000410
TGTACCG408.3200575E-944.00000415
TCGGTTA207.858257E-444.00000424
CGGAGAT207.858257E-444.00000419
Produced by FastQC (version 0.11.5)