FastQCFastQC Report
Thu 26 May 2016
SRR1546197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546197_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1683877
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283881.6858713552118119No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51440.3054854956745653No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC24320.14442860137646635No Hit
GAGAGAGGACGAGAGAAAGAGAGAAAGAGAAAGAGAGAAAGAGAGAAAGA20000.11877352086880455No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.10060117217587745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG408.3164196E-944.0000041
AGCGTCG207.8575255E-444.00000417
GGTACGT207.8575255E-444.00000434
ACGCCTA207.8575255E-444.00000430
CTATACG207.8575255E-444.0000041
CCGGATG800.044.0000041
TCGGCCA207.8575255E-444.00000444
TAACCCG207.8575255E-444.00000437
TGCGCAA207.8575255E-444.0000041
GTTCGGC408.3164196E-944.00000432
CTCGGAA207.8575255E-444.00000430
CCGAATC207.8575255E-444.00000418
CGGTCTA800.044.00000431
ACCGTCA207.8575255E-444.00000415
CCAACGT207.8575255E-444.00000418
ATACGTC207.8575255E-444.0000049
CACGTCG408.3164196E-944.0000041
AGGATCG207.8575255E-444.00000412
GTAAGCG800.044.0000041
ATACGAG207.8575255E-444.0000041
Produced by FastQC (version 0.11.5)