##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546197_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1683877
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93104128151878	31.0	31.0	34.0	30.0	34.0
2	32.28937624303913	33.0	31.0	34.0	30.0	34.0
3	32.16912636730593	33.0	31.0	34.0	30.0	34.0
4	35.92369216991502	37.0	35.0	37.0	35.0	37.0
5	35.9317277924694	37.0	35.0	37.0	35.0	37.0
6	36.0048127030656	37.0	35.0	37.0	35.0	37.0
7	36.21226609782068	37.0	35.0	37.0	35.0	37.0
8	36.10719488418691	37.0	36.0	37.0	35.0	37.0
9	37.87528423988213	39.0	38.0	39.0	35.0	39.0
10	37.60410291250489	39.0	37.0	39.0	35.0	39.0
11	37.394459334024994	39.0	37.0	39.0	35.0	39.0
12	36.867971948069844	39.0	35.0	39.0	33.0	39.0
13	36.71568647828791	39.0	35.0	39.0	33.0	39.0
14	37.895256601283826	40.0	35.0	41.0	33.0	41.0
15	38.05857019247843	40.0	35.0	41.0	33.0	41.0
16	38.15203188831488	40.0	35.0	41.0	34.0	41.0
17	38.060515108882655	40.0	35.0	41.0	34.0	41.0
18	37.8507824502621	39.0	36.0	41.0	34.0	41.0
19	37.57097282046135	38.0	36.0	41.0	34.0	41.0
20	37.23465906357768	38.0	35.0	41.0	34.0	41.0
21	37.15572871415193	38.0	35.0	41.0	33.0	41.0
22	37.08501095982664	38.0	35.0	41.0	33.0	41.0
23	37.023668593371134	38.0	35.0	41.0	33.0	41.0
24	36.962807259675145	38.0	35.0	41.0	33.0	41.0
25	36.93325700155059	38.0	35.0	41.0	33.0	41.0
26	36.861082489991844	38.0	35.0	41.0	33.0	41.0
27	36.788699530903976	38.0	35.0	41.0	33.0	41.0
28	36.72318286905754	38.0	35.0	41.0	33.0	41.0
29	36.640635865921325	38.0	35.0	41.0	33.0	41.0
30	36.4124107639691	38.0	35.0	41.0	32.0	41.0
31	36.06712010437817	38.0	35.0	41.0	31.0	41.0
32	35.64014414354492	38.0	35.0	41.0	29.0	41.0
33	35.152385833407074	38.0	35.0	41.0	24.0	41.0
34	34.6110624469602	38.0	35.0	41.0	21.0	41.0
35	34.312445623997476	38.0	34.0	41.0	18.0	41.0
36	34.120264722423315	38.0	34.0	41.0	18.0	41.0
37	34.06721809253289	38.0	34.0	41.0	17.0	41.0
38	34.00157612462193	38.0	34.0	40.0	17.0	41.0
39	33.934748203105094	37.0	34.0	40.0	16.0	41.0
40	33.8051205640317	37.0	34.0	40.0	15.0	41.0
41	33.66685690225592	37.0	34.0	40.0	15.0	41.0
42	33.57478248114322	37.0	34.0	40.0	14.0	41.0
43	33.42483566198719	37.0	33.0	40.0	12.0	41.0
44	33.271522207382134	37.0	33.0	40.0	10.0	41.0
45	33.2291378764601	37.0	33.0	40.0	10.0	41.0
46	33.14238569681752	36.0	33.0	40.0	10.0	41.0
47	33.13071382292175	36.0	33.0	40.0	10.0	41.0
48	33.069653543578305	36.0	33.0	40.0	10.0	41.0
49	32.99027007317043	36.0	33.0	40.0	10.0	41.0
50	32.8656154814158	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	3.0
11	3.0
12	3.0
13	5.0
14	5.0
15	35.0
16	78.0
17	231.0
18	553.0
19	1185.0
20	2317.0
21	3940.0
22	6336.0
23	10482.0
24	16530.0
25	28221.0
26	45355.0
27	53543.0
28	47608.0
29	39693.0
30	37650.0
31	39146.0
32	46376.0
33	59370.0
34	107651.0
35	230915.0
36	97684.0
37	115313.0
38	196417.0
39	497224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.0466174192058	23.88220754841357	28.396967236918137	21.674207795462493
2	34.80753047877012	25.557329899986758	29.453041997723112	10.182097623520008
3	23.55154206631482	23.455632448213258	42.53725183015149	10.45557365532043
4	21.73953323194034	26.398008880696157	41.2676222788244	10.594835608539103
5	19.44708550565154	28.659219170996458	41.0877397814686	10.805955541883403
6	17.572898733102242	37.628401599404235	35.81146366391369	8.987236003579833
7	76.00341355098978	4.50080379980248	18.051259088401352	1.4445235608064009
8	76.56164909907315	4.173879683611095	17.228396135822273	2.036075081493482
9	72.41817543680446	6.088152519453618	18.84739799878495	2.6462740449569653
10	38.54960902726268	29.914595899819286	23.42017855223392	8.115616520684112
11	33.4040431694239	20.59895111103721	35.30038120361523	10.69662451592367
12	31.727851856162893	20.838339142348282	35.877026647433276	11.556782354055551
13	15.933764758352304	36.58390725688396	34.8152507576266	12.667077227137135
14	11.636182452756346	35.10951215557906	38.177254039338976	15.077051352325615
15	9.729095414926388	24.584159056748206	52.862649706599704	12.824095821725695
16	12.664226662636285	22.64625029025279	47.479180486460706	17.21034256065021
17	12.455779133511532	23.3096597910655	38.90052539466956	25.334035680753402
18	15.698415026750768	25.778129875281863	42.89173140318444	15.63172369478293
19	20.205276276117555	26.739660913475273	35.86722783196159	17.187834978445576
20	21.03336526361486	24.106986436657785	39.96669590474839	14.892952394978968
21	16.72259909720247	28.79996579322599	39.02618777974876	15.451247329822785
22	18.110942782637924	25.248637519248735	35.12020177245726	21.52021792565609
23	13.799463975100318	29.310751319722282	36.473150948673805	20.416633756503593
24	13.562926508290094	25.15736006846106	48.476818674998235	12.802894748250615
25	12.30844058087378	29.097255915960606	42.639337671338225	15.954965831827383
26	12.52775588715803	32.7411681494551	38.02356110333475	16.70751486005213
27	13.052972396439882	34.39936527430448	36.82157307214244	15.726089257113198
28	11.095169065198943	30.72552211355105	43.21924938698017	14.960059434269843
29	12.132002515623173	24.883884036660636	41.77264728955856	21.211466158157634
30	14.773881940307993	32.491684368870175	37.25770944077269	15.476724250049143
31	18.783141523994924	30.97559976174032	35.180182400495994	15.061076313768762
32	19.592642455476263	28.478624032515437	35.08914249675006	16.83959101525824
33	20.178195913359467	31.24177122200731	31.52463036195637	17.05540250267686
34	14.873057830233444	28.276115179434125	35.24224156514995	21.60858542518248
35	17.433696166644	28.27213626648502	34.920424710355924	19.373742856515054
36	22.15244937724074	30.062528320061382	31.026316055151298	16.758706247546584
37	15.885364548598266	32.58616870472131	36.060591123936014	15.467875622744417
38	15.820395432683029	31.557590013997462	34.972922606579935	17.649091946739578
39	16.208012818038373	33.303798317810624	32.78826185047958	17.699927013671427
40	19.01029588265651	28.898072721463624	32.14450936737065	19.94712202850921
41	14.764498832159356	29.62544176326418	34.061038900109686	21.549020504466775
42	16.95765189500183	28.944275621081587	31.544584313462327	22.553488170454255
43	17.1298735002616	29.69741851691068	31.88795856229404	21.284749420533686
44	14.236609918657955	31.325922261542853	33.38011030496883	21.057357514830358
45	15.405935231611334	36.433480592703624	29.312117215212275	18.848466960472766
46	18.04508286531617	33.40968491166516	31.57136774241824	16.973864480600422
47	16.90586663990303	31.229418775836955	32.5323049130073	19.33240967125271
48	17.271748470939386	27.551715475655286	35.547073806459736	19.62946224694559
49	18.328357712588268	28.896884986254932	33.55726101134465	19.217496289812143
50	16.610061186179276	32.21684244157976	32.12200178516602	19.051094587074946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6596.0
1	7921.0
2	9246.0
3	27079.0
4	44912.0
5	33252.0
6	21592.0
7	23582.5
8	25573.0
9	27936.0
10	30299.0
11	30839.5
12	31380.0
13	31124.5
14	30869.0
15	27241.0
16	23613.0
17	21892.5
18	20172.0
19	19082.0
20	17992.0
21	19463.0
22	20934.0
23	21447.0
24	21960.0
25	24155.5
26	26351.0
27	33856.5
28	41362.0
29	42998.0
30	44634.0
31	58601.0
32	72568.0
33	80831.5
34	89095.0
35	93734.5
36	98374.0
37	101882.0
38	105390.0
39	106768.0
40	108146.0
41	124834.0
42	141522.0
43	138118.0
44	134714.0
45	123791.5
46	112869.0
47	104555.5
48	96242.0
49	89564.5
50	82887.0
51	76425.5
52	69964.0
53	57237.5
54	44511.0
55	36870.5
56	29230.0
57	24432.5
58	19635.0
59	18364.0
60	17093.0
61	15601.5
62	14110.0
63	11873.5
64	9637.0
65	8528.5
66	7420.0
67	6227.5
68	5035.0
69	4076.5
70	3118.0
71	2578.0
72	2038.0
73	1672.5
74	1307.0
75	1004.0
76	701.0
77	545.0
78	389.0
79	293.0
80	197.0
81	151.0
82	105.0
83	75.0
84	45.0
85	32.0
86	19.0
87	20.0
88	21.0
89	11.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1683877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.597133908379703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75171308514992	18.0216014000781
2	8.39220440642889	3.7927953351713564
3	3.0093248133492674	2.0400634734318945
4	1.5035763757525011	1.35906066817422
5	0.9709003480923711	1.0969782588767887
6	0.6522331201087906	0.8843159492747188
7	0.48348906875173386	0.764782706087451
8	0.38990753431054714	0.704863421176127
9	0.30800009264137423	0.6263927403489438
>10	3.1613476642896527	15.974022039272526
>50	0.7703397896167532	12.168133352141174
>100	0.5141743900020073	23.644126632591153
>500	0.07349124956906436	11.549811445360875
>1k	0.018769347911523633	5.3699478662788875
>5k	2.6435701283836097E-4	0.30728768451535265
>10k+	2.6435701283836097E-4	1.695817027220418
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28388	1.6858713552118119	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5144	0.3054854956745653	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	2432	0.14442860137646635	No Hit
GAGAGAGGACGAGAGAAAGAGAGAAAGAGAAAGAGAGAAAGAGAGAAAGA	2000	0.11877352086880455	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1694	0.10060117217587745	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06752274661391539	0.0
2	0.0	0.0	0.0	0.281374470938198	0.0
3	0.0	0.0	0.0	0.41267859825866143	0.0
4	0.0	0.0	0.0	0.6442869639528302	0.0
5	0.0	0.0	0.0	1.1279327409306024	0.0
6	0.0	0.0	0.0	1.5390672834179693	0.0
7	0.0	0.0	0.0	1.79781539863066	0.0
8	0.0	0.0	0.0	2.428027700360537	0.0
9	0.0	0.0	0.0	2.6618333761907786	0.0
10	0.0	0.0	0.0	3.127960058840402	0.0
11	0.0	0.0	0.0	3.7068621995549558	0.0
12	0.0	0.0	0.0	4.130943055817022	0.0
13	0.0	0.0	0.0	4.28861490477036	0.0
14	0.0	0.0	0.0	4.3461012888708614	0.0
15	0.0	0.0	0.0	4.435597136845506	0.0
16	0.0	0.0	0.0	4.649627021451091	0.0
17	0.0	0.0	0.0	4.902911554703818	0.0
18	0.0	0.0	0.0	5.227519587238261	0.0
19	0.0	0.0	0.0	5.405264161218426	0.0
20	0.0	0.0	0.0	5.6285583804517785	0.0
21	0.0	0.0	0.0	5.88273371511102	0.0
22	0.0	0.0	0.0	6.147598666648455	0.0
23	0.0	0.0	0.0	6.456944301751256	0.0
24	0.0	0.0	0.0	6.672933949451178	0.0
25	0.0	0.0	0.0	6.874730161407276	0.0
26	0.0	0.0	0.0	7.086384575595486	0.0
27	0.0	0.0	0.0	7.290734418250264	0.0
28	0.0	0.0	0.0	7.538377209261721	0.0
29	0.0	0.0	0.0	7.766719303131999	0.0
30	0.0	0.0	0.0	8.05029108420627	0.0
31	0.0	0.0	0.0	8.33142800810273	0.0
32	0.0	0.0	0.0	8.581505656291997	0.0
33	0.0	0.0	0.0	8.832355332366912	0.0
34	0.0	0.0	0.0	9.085342931817467	0.0
35	0.0	0.0	0.0	9.412860915613194	0.0
36	0.0	0.0	0.0	9.673687567441089	0.0
37	0.0	0.0	0.0	9.941224923198073	0.0
38	0.0	0.0	0.0	10.19130257138734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	40	8.3164196E-9	44.000004	1
AGCGTCG	20	7.8575255E-4	44.000004	17
GGTACGT	20	7.8575255E-4	44.000004	34
ACGCCTA	20	7.8575255E-4	44.000004	30
CTATACG	20	7.8575255E-4	44.000004	1
CCGGATG	80	0.0	44.000004	1
TCGGCCA	20	7.8575255E-4	44.000004	44
TAACCCG	20	7.8575255E-4	44.000004	37
TGCGCAA	20	7.8575255E-4	44.000004	1
GTTCGGC	40	8.3164196E-9	44.000004	32
CTCGGAA	20	7.8575255E-4	44.000004	30
CCGAATC	20	7.8575255E-4	44.000004	18
CGGTCTA	80	0.0	44.000004	31
ACCGTCA	20	7.8575255E-4	44.000004	15
CCAACGT	20	7.8575255E-4	44.000004	18
ATACGTC	20	7.8575255E-4	44.000004	9
CACGTCG	40	8.3164196E-9	44.000004	1
AGGATCG	20	7.8575255E-4	44.000004	12
GTAAGCG	80	0.0	44.000004	1
ATACGAG	20	7.8575255E-4	44.000004	1
>>END_MODULE